Plasma-vernietiging van rubberskroot : teoretiese en eksperimentele onderbou

Get full text

Monitoring of selected pesticide concentrations in South African aquatic systems using the Chemcatcher® passive sampler

Get full text

Investigation of the genetic aetiology of aminoglycoside-induced hearing loss in South African populations by Human, Hannique

Get full text

An assessment of polymeric materials and surface treated steels as cavitation erosion resistant materials by Barletta, A

“…The effect of prior immersion (3 weeks at 70°C) in either a dilute or concentrated form of hydraulic fluid has been investigated for both polyacetal and ultra high molecular weight polyethylene samples in order to simulate service conditions. The polyacetal samples showed improved erosion resistance relative to the samples stored in air or water (3 weeks at 70°C). …”
Get full text

Evaluating the use of capillary electrophoresis in the forensic DNA profiling of burnt teeth by Geldenhuys, Adriaan

“…The aim of this study was thus to evaluate the success of capillary electrophoresis in the forensic DNA profiling of teeth burnt at different temperatures, using an optimised DNA extraction protocol. Tooth samples from 25 donors (n = 100 [4 teeth per donor]) were subjected to three burning conditions, one tooth was left unburnt to act as a control and three teeth were each burnt in a muffle furnace at 100 ˚C, 200 ˚C, and 300 ˚C. …”
Get full text

Investigating the air and liquid porosity of sea ice by Swait, Hayley

“…In this study, non-destructive X-ray computer tomography (CT) analysis was used to investigate the brine and air inclusions within pancake ice from the Antarctic MIZ during winter 2019 and 2022 expeditions. Sampling protocols from the 2019 expedition were optimised during the 2022 expedition to store samples at the CT analysis temperature (-10°C) to minimise the impact of low temperature (-18°C) storage on these inclusions. …”
Get full text

Towards a complete human cell atlas: a single-nucleus RNA sequencing study of the paediatric and adult human brain by Steyn, Christina

“…To address this, we present the first paediatric brain cell atlas comprising of 6 single nucleus RNA sequencing (snRNA-seq) datasets generated from antemortem human brain tissue samples obtained during elective surgeries to treat epilepsy. …”
Get full text

Development of a multiplex real-time PCR to distinguish between Mycoplasma species found in South African poultry

Get full text

The experiences of employees regarding workplace violence in the South African Police Service

Get full text
Get full text

Development of a real-time PCR assay to detect the fusion gene of the D26 strain of a commercial avian avulavirus 1

Get full text

Metabarcoding pollen to identify allergenic species of grasses (Poaceae) and ragweed (Ambrosia spp.) across six monitored sites in South Africa by Sidla, Siyavuya

“…Results and Discussion The DNA extraction protocol was optimised by altering the bead beating method using the samples from weeks with high grass pollen concentrations. …”
Get full text

Metabarcoding pollen to identify allergenic species of grasses (Poaceae) and ragweed (Ambrosia spp.) across six monitored sites in South Africa by Sidla, Siyavuya

“…Results and Discussion The DNA extraction protocol was optimised by altering the bead beating method using the samples from weeks with high grass pollen concentrations. …”
Get full text

The house dust microbiota in the Drakenstein Child Health Study by Duyver, Menna

“…Aims: The aims of the studies described in this MSc dissertation were to: 1) evaluate the performance of ten commercial nucleic acid extraction kits on dust samples; 2) optimise dust removal from electrostatic dustfall collectors (EDC); 3) determine the bacterial composition of house dust using 16S rRNA gene sequencing and 4) determine those factors influencing the bacterial composition of house dust by performing bioinformatic and data analysis on the sequenced dust samples. …”
Get full text

The systems engineering of automated fire assay laboratories for the analysis of the precious metals by McIntosh, Keith Shearer

Get full text

Diving into the biodiversity of the Great African Seaforest using environmental DNA metabarcoding by Courtaillac, Kira-Lee

Get full text

Phylogeny reconstruction of the Schoeneae (Cyperaceae) with a focus on southern African genera by Henning, Jessica

“…Further research needs to be done including the sampling of more taxa and more sequences. The monophyly of this tribe is still yet to be resolved. …”
Get full text

Studies on immunity in the male genital tract by Olivier, Abraham Jacobus

“…A challenge that hampers studies at this mucosal surface is obtaining samples with sufficient immune cells. Therefore, the first aim of this study was to establish an optimised method to isolate immune cells from the male genital tract. …”
Get full text

Towards molecular autopsies: Internal validation of the Qubit 1 X dsDNA HS Assay Kit by Naidoo, Adele

“…The Qubit™ 1X dsDNA HS Assay workflow was optimised and then used to assess the DNA concentration of control Lambda DNA, extracted DNA from forensic samples and DNA sequencing libraries. …”
Get full text

Detection and characterization of E. coli O157:H7 and induced shiga toxin-2 coding bacteriophages

Get full text
Get full text

Exploring the gene regulatory dynamics of the maturing human brain by Fillmore, Stephanie

“…Bio-banked pediatric and adult brain tissue samples, obtained during surgery to treat epilepsy, were used to optimise and generate a nuclei isolation protocol. …”
Get full text