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Publisher’s Note: Sparse dimensionality reduction for analyzing single-cell-resolved interactions
Published in
Bioinformatics Advances : Journal of the International Society for Computational Biology
(2026)
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Cell type annotation using large language models (LLMs) and CytoAnalyst
Published in
Bioinformatics Advances : Journal of the International Society for Computational Biology
(2026)
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Research on the Role of Autophagy in Vascular Endothelial Cells in the Healing of Chronic Refractory Wounds
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Biologics: Targets & Therapy
(2026)
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Saccharomyces cerevisiae yeast cells as a test system for assessing Zeocin toxicity
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BioRisk
(2022)
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Application Strategies of Bone Marrow Mesenchymal Stromal Cells in Bone‐Related Diseases
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Cell Proliferation
(2026)
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PTEN restrains SHH medulloblastoma growth through cell autonomous and nonautonomous mechanisms
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eLife
(2026)
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Revealing global stoichiometry conservation architecture in cells from Raman spectral patterns
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eLife
(2026)
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Impacts of Phenolic Compounds Alone and in Combination with Natural Substances or Drugs on Cancer Cells
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European Journal of Biology
(2026)
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Molecular evolution of stem cell regulators driving powerful regenerative abilities in plants
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Journal of Systematics Evolution
(2026)
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Cell type-agnostic transcriptomic signatures enable uniform comparisons of neural maturation
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PLoS Biology
(2026)
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Unannotated noncoding transcripts as a source of intratumor heterogeneity in malignant cell states
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Science China Life Sciences
(2026)
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Alginate-Based Hydrogels with Amniotic Membrane Stem Cells for Wound Dressing Application
Published in
Stem Cells and Cloning: Advances and Applications
(2025)
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Small regulatory RNAs modulate lactococcal susceptibility to cell wall-targeting antimicrobials
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Nucleic Acids Research
(2026)
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Discovering paracrine regulators of cell type composition from spatial transcriptomics using SPER
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Bioinformatics Advances : Journal of the International Society for Computational Biology
(2026)
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Safety and Efficacy of Mesenchymal Stem Cell Therapy in Multiple System Atrophy: Systematic Review
Published in
Biologics: Targets & Therapy
(2026)
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Determination of Cell Viability—Acridine Orange/Propidium Iodide (AO/PI) Staining Method
Published in
Cell Proliferation
(2026)
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Efficient Basis Change for Sparse-Grid Interpolating Polynomials with Application to T-Cell Sensitivity Analysis
Published in
Computational Biology Journal
(2013)
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Uev1A counteracts oncogenic Ras stimuli in both polyploid and diploid cells
Published in
eLife
(2026)
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An interpretable Graph-Regularized Optimal Transport Framework for Diagonal Single-Cell Integrative Analysis
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GigaScience
(2026)
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Cytotoxic effects of three different oral antiseptic solutions on epithelial cells of buccal mucosa
Published in
Mediterranean Journal of Biosciences
(2017)
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FRELIP Discipline
Natural Sciences
180 results
180
Life Sciences & Biology
22 results
22
Technology & Engineering
1 results
1
FRELIP Sub-Discipline
Natural Sciences — Life Sciences
179 results
179
Biological Sciences
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Natural Sciences
180 results
180
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179 results
179
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175
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22 results
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22 results
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— — — — — — Genetics
19 results
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Microbiology
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NATURAL SCIENCES::Biology::Organism biology::Morphology
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PLOS Computational Biology Atom
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A nationwide equine influenza virus surveillance was carried out between January 1989 and January 1995 during which nasopharyngeal swabs, tracheal washes and respiratory tract tissue homogenates were obtained from symptomatic and asymptomatic equine species in various parts of Nigeria for virus isolation and characterisation. Three equine influenza viruses were independently isolated in 10-11 day old embryonated hen eggs and Madin Darby canine kidney monolayer cells with TPCK-trypsin incorporated into the culture medium. The virus isolates were characterized antigenically, immunogenically and genetically which made them the first equine influenza viruses isolated and characterized in tropical Africa. Antigenic analyses with mono-specific antisera raised against a number of respiratory disease viruses including Paramyxoviruses, Adenoviruses, Herpes viruses, Alphavirus, Rhinoviruses, Equine arteritis viruses, African horse sickness viruses and Influenza viruses showed that the three virus isolates were influenza A viruses while haemagglutination and neuraminidase inhibition assays using influenza virus specific Mabs and polyclonal antisera showed the three viruses to be antigenically H3N8, members of equine-2 subtype of influenza A viruses. The three isolates were respectively named A/Equine/Ibadan/4/91, A/Equine/Ibadan/6/91 and A/Equine/Ibadan/9/91 in accordance with the recommendations of the World Health Organisation and the International Committee on Virus Taxonomy. Their antigenic cross-reactivity with panels of Mabs and polyclonal antisera indicated that the three viruses were antigenically divergent although they were all H3N8 viruses and were representatives of a homogenous population. They were reactive with Mabs and antisera directed against H3 equine viruses isolated in 1963, 1976, 1979, 1981 and 1986. This broad reactivity suggested that these isolates were similar to but not still different from those isolated in Europe and USA between 1963 and 1987. Virus protein synthesis and cleavability of the HA polypeptide in tissue culture were investigated by pulse-chase experiments. The results showed some heterogeneity in the non-glycosylated polypeptides particularly those of the ribonucleoprotein (RNP) complex while the HA glycoproteins of the three viruses were not cleaved in any of the cell types used in contrast to equine H7 and pathogenic avian H5 and H7 HAs. The molecular weights of the polypeptides were within the range previously determined for influenza A viruses. The heterogeneity of the RNP complex and antigenic divergence of the viruses’ HAs were confirmed by sequence analysis carried out in molecular studies. Virus infectivity was investigated by plaque assay using chicken embryo fibroblast (CEF) monolayer cells and virus titration in embryonated hen eggs. The results showed that the viruses were infectious with plaguing efficiency being comparable to efficiency of virus infectivity in embryonated hen eggs. Infectivity, antigenicity and immunogenicity of the three viruses were confirmed in-vitro using peripheral blood lymphocytes in lymphoproliferation assays and in-vivo in an equine model in which the viruses induced haemagglutination inhibiting and protective neutralising antibodies following experimental infections. The proliferating cells were also characterised and the immunoglobulin isotypes produced were determined. Molecular characterisation and genetic analyses of the three viruses were accomplished to determine the origin of the genes encoding the virus non-glycosylated polypeptides as well as those of the surface HA glycoprotein. Reverse transcription (RT) results showed the eight RNA segments of the three isolates and confirmed them as influenza A virus RNAs. No subgenomic RNAs or defective interfering particles were observed in the RNA transcripts. Nucleotide sequence analyses were carried out using three sequencing strategies of cDNA, vRNA and plasmid DNA sequencing with the dideoxy chain termination procedure. Partial nucleotide sequences were obtained from cDNAs, vRNAs and plasmid DNA clones of RNA segments 1, 2, 3, 5, 7 and 8 with the method of multiplex RT/PCR and cycle sequencing using radiolabelled segment specific oigonucleotides of 18, 24 or 25 mers. Complete nucleotide sequences of segment 4 (HA genes) were also determined on the same PCR products (cDNA) and vRNA using end-labelled oligonucleotides of both plus and minus sense. The partial nucleotide sequence data were analysed using a programme for "best-local-homology- rapid-search" on a digital array processor while the complete HA nucleotide and deduced amino acid sequence analyses were carried out using the University of Wisconsin Genetics Computer Group (GCG) package of programmes. Phytogenetic analysis was done with the distances, neighbour joining and DNAPARS of the PHYLIP package. Analyses of the viruses’ gene sequences confirmed that their genomes were similar to each other and to those of other H3N8 influenza viruses isolated from equines and also revealed the origin, evolution and genetic relatedness of the genes. Comparison of the partial cDNA sequences with virus DNA sequences in the database (EMBL sequence library) showed that for segments 1, 2, 5, and 7, the closest related sequences were from equine H3 viruses isolated in 1986 in USA (Tennesse/5/86 for segments 2, 5 and 7, Kentucky/2/86 for segments 1 and 5. Segment 5 was equally related to both viruses). The nucleotide sequence for segment 3 was most closely related to an equine-1 virus isolated in U.K. in 1973 (London/1416/73, H7N7) probably due to genetic exchange while segment 8 sequence was most closely related to an equine H3N8 virus isolated in U.K. in 1976 (Newmarket/76). The complete nucleotide and deduced amino acid sequences as well as phytogenetic analysis of the HA genes (RNA segment 4) showed a closer relationship albeit with nucleotide and amino acid substitutions between the three Ibadan viruses and those that were isolated in Europe in 1989 and 1991, the prototypic European strain, Suffolk/89 and Arundel/12369/91 isolated in U.K., Taby/91 isolated in Sweden, Hong Kong/92 isolated in the Far East in 1992 and Laplata/1/93 isolated in South America. These findings group the Ibadan viruses with those predominating and contemporarily causing disease in the Western Hemisphere rather than with viruses previously isolated from the north and south of African continent. Variation was observed in the nucleotide sequences of the Ibadan viruses HA genes. Some of the base changes resulted in amino acid changes which mapped to antigenic sites or within signal sequence in the HA1 domain as a result of a process of antigenic or genetic drift. The Ibadan viruses also showed some variation from the prototypic European virus (Suffolk/89) and these base changes also resulted in amino acid changes resulting in antigenic drift. Phytogenetic analysis showed the evolutionary lineages in equine H3 viruses isolated since 1963 along two paths one of which included the Ibadan viruses as well as viruses isolated in Europe between 1989 and 1991 and the Far East in 1992 and S. America in 1993 which form the 1989/93 cluster while the other lineage included viruses isolated in South America in 1987 and 1988 (Brazil/87, Laplata/88) and in the Far East in 1971 (Tokyo/3/71) all of which are very close to the original prototype equine-2 virus (Miami/63). These results demonstrate a faster evolutionary rate for recent equine H3 HA genes away from the original prototype virus. Overall, the results of these studies have (i) confirmed the occurrence of equine-2 H3N8 influenza viruses of distinct lineages in Nigerian equine populations in a tropical environment (ii) indicated antigenic drift among equine H3N8 viruses as earlier reported and confirmed that drift strains can co-circulate in equine populations, (iii) showed the origin, evolution and genetic relatedness of the viruses genes as well as their biological characteristics, (iv) provided the hitherto unavailable information on the status of equine influenza virus in this part of the world and (v) served to re-emphasise the potential of influenza virus for rapid global spread and the need for better control strategies.
1 results
1
Active ingredients
1 results
1
African four-toed hedgehog
1 results
1
Albizia sama
1 results
1
Alchornea
1 results
1
BACKGROUND: Polymerase Chain Reaction (PCR) has become an important diagnostic and research tool of modern molecular biology globally. Real-time PCR allows for rapid and reliable quantification of mRNA transcription. Reference genes are used as internal reaction control to normalise mRNA levels between different samples in order to allow for an exact comparison of mRNA transcription level. METHODS: In this study, twelve commonly used human reference genes were investigated in Human Embryonic Kidney Cell Lines (HEK293) using real-time qPCR with SYBR green. The genes included beta-2-microglobulin (B2M), glyceraldehyde-3- phosphate dehydrogenase (GAPDH), succinate dehydrogenase complex subunit A (SDHA), and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta polypeptide (YWHAZ). The stability of these reference genes was investigated using the geNorm application. RESULTS: The range of expression stability in the genes analysed was (from the most stable to the least stable): UBC, TOP1, ATP5B, CYC1, GAPDH, SDHA, YWHAZ, CTB, 18S, EIFA-2, B2M and RPL13A. The optimal number of reference targets in the experiment was calculated to be 2 (geNorm V<0.15) when comparing a normalization factor based on the 2 or 3 most stable targets). CONCLUSION: The expression stability varied greatly between the 12 candidate reference genes. UBC, TOP1, ATP5B, CYC1 and GAPDH respectively showed the highest stability in HEK293 cells based on both expression stability and expression level. Overall, our data suggest that UBC and TOP1show the least variation and the highest expression stability. This report validates the need for rational selection of reference genes for data normalization to ensure accuracy of quantitative PCR assays.
1 results
1
Biocontrol powder Phule Trichoderma has been successfully used on a number of horticultural crops. The present investigation was carried out to explore the nematicidal properties of Phule Trichoderma against the root-knot nematode (Meloidogyne spp.) infesting the tissue culture banana (Dwarf Cavendish - var. Basarai). In vitro tests slowed that the various concentrations of Phule Trichoderma prevented nematode egg hatching and also resulted in 100% mortality of nematode juveniles. Tissue culture banana plants were also dipped into various concentrations of Phule Trichoderma before planting out into plastic bags. Plant were inoculated with 250 nematode juveniles and alter ten days, the roots were stained with cotton blue lactophenol and nematodes were counted under a dissecting microscope. The results indicated that the higher the concentration of Phule Trichoderma in banana plants, the lower the ability of the nematode to penetrate the roots. Furthermore, the nematode juveniles that penetrated the treated roots were found dead. This could have resulted from the toxic metabolites produced front Phule Trichoderma. Also, root zone treatment of plants treated with Phule Trichoderma prevented the development of giant cells and roots knots in treated plants while the development of giant cells and root knots were observed in untreated plants exposed to nematode infestation.
1 results
1
Bioengineering
1 results
1
Biological value
1 results
1
Chemistry
1 results
1
Complementary diet
1 results
1
Conventional vegetable protein ingredients in poultry feed are costly and relatively scarce. Moringa oleifera Seed Meal (MSM) is an oil seed rich in protein, which can be exploited as an alternative vegetable protein source. However, the potential of MSM as a protein source for broiler production has not been adequately documented. Therefore, this study was conducted to investigate the utilisation of MSM as a protein source in the diets of broiler chickens. Moringa seeds were harvested and apportioned into four parts representing T1, T2, T3 and T4. The T1 was raw, while T2, T3 and T4 were soaked in water (200 g/L) for 1, 2, and 3 hours, respectively. The seeds were then sun-dried, milled and analysed for Crude Protein (CP, %), alkaloid (%), saponin (%) and tannin (%), using standard procedures. The protein quality of MSM was assessed using forty weanling albino rats (42.35±0.83 g) randomly allotted to four diets containing 10.0% casein (D1), 10.0% raw MSM (D2), 10.0% 3-hour Water-soaked MSM (WMSM, D3) and a protein free diet (D4) fed for 21 days. Protein quality: Biological Value (BV, g), Protein Efficiency Ratio (PER) and Net Protein Utilisation (NPU, g) were determined using standard procedures. Two hundred and fifty 1-day old broiler chicks were randomly allotted to five diets wherein Full-fat Soyabean (FS) was replaced with 3-hour WMSM at 0.0, 25.0, 50.0, 75.0 and 100.0%. Daily Weight Gain (DWG, g/bird), Feed Intake (FI, g/bird), and Feed Conversion Ratio (FCR) were measured. Blood (5mL) samples were collected for determination of Packed Cell Volume (PCV, %), Red Blood Cell (RBC x106/µL) and White Blood Cell (WBCx103/µL) counts, Total Cholesterol (TC, mg/dL), triglycerides (mg/dL) and immunoglobulins: IgG, IgA and IgM (IU/dL) using standard procedures. Data were analysed using descriptive statistics and ANOVA at α0.05. The CP of T1 (35.4±0.7), T2 (35.3±0.4), T3 (35.7±0.8) and T4 (36.1±0.7%) were similar, while alkaloid, saponin and tannin content ranged from 0.05±0.10 (T4) to 0.07±0.04 (T1), 0.42±0.50 (T4) to 1.17±0.28 (T1) and 0.05±0.02 (T4) to 0.06±0.02 (T1), respectively. The BV ranged from 54.87±5.54 (D4) to 65.69±5.70 (D1), PER from 0.63±0.08 (D4) to 1.10±0.33 (D1) and NPU from 15.86±1.22 (D4) to 44.37±4.31 (D1). The birds fed 100.0%WMSM had least DWG (0.63±0.11), while birds on 100.0%FS had the highest (1.30±0.15). The FI was least in 100.0%WMSM (2.19±0.86) and highest in 100.0%FS (30.00±7.86). The FCR ranged from 2.45±0.32 (100.0%FS) to 3.48±0.26 (100.0%WMSM). The PCV ranged from 29.7±0.54 (100.0%FS) to 34.5±0.86 (100.0%WMSM), RBC from 2.98±0.06 (75.0%WMSM) to 3.44±0.08 (25.0%WMSM) and WBC from 1.25±0.52 (100.0%FS) to 1.53±0.64 (100.0%WMSM). Birds fed 100.0%WMSM had least TC (157.67±10.72) compared with 100.0%FS (206.33±13.52) and triglycerides, which ranged from 56.00±3.26 (100.0%WMSM) to 85.36±5.35 (100.0%FS). The IgG, IgA and IgM recorded for birds on WMSM diets ranged from 0.62±0.36 (100.0%FS) to 0.73±0.40 (100.0%WMSM), 0.31±0.18 (100.0%FS) to 0.49±0.15 (100.0%WMSM) and 0.09±0.04 (100.0%FS) to 0.15±0.08 (100.0%WMSM), respectively. Three hours water-soaked Moringa oleifera seed meal at 100.0% inclusion reduced growth, enhanced immunoglobulins profile and lowered total cholesterol of broiler chickens.
1 results
1
Full-fat soyabean
1 results
1
Gene expression
1 results
1
Haematological
1 results
1
Histological
1 results
1
Human cell lines
1 results
1
Immunoglobulins
1 results
1
Immunoreactivities
1 results
1
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Stem Cells and Cloning: Advances and Applications
25 results
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eLife
18 results
18
Cell Proliferation
15 results
15
Bioinformatics Advances : Journal of the International Society for Computational Biology
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14
BioDiscovery
12 results
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PLoS Computational Biology
9 results
9
OncoTargets and Therapy
8 results
8
Nucleic Acids Research
7 results
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Protein & Cell
6 results
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Science China Life Sciences
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GigaScience
5 results
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Molecular Biomedicine
5 results
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PLoS Biology
5 results
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Biologics: Targets & Therapy
4 results
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PNAS Nexus
4 results
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Reproductive Medicine and Biology
4 results
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BioRisk
3 results
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Bioactive Compounds in Health and Disease
3 results
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EJNMMI Research
3 results
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Journal of Bioresource Management
3 results
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Open Journal of Cell Biology
3 results
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PLOS Computational Biology Atom
3 results
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PLoS Genetics
3 results
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Aging Cell
2 results
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Biology Methods and Protocols
2 results
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Computational Biology Journal
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Environmental Epigenetics
2 results
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European Journal of Biology
2 results
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Karbala International Journal of Modern Science
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Proceedings of the Latvian Academy of Sciences. Section B: Natural, Exact and Applied Sciences
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FRELIP Feed Integration
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Catalog
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