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The yield of nasopharyngeal bacteria from culture compared to polymerase chain reaction in South African children with lower respiratory tract infection
Published 2023“…Culture of respiratory tract specimens is labour-intensive and slow. Polymerase chain reaction (PCR) is comparatively, a rapid, sensitive method of detecting low levels of nucleic acid for clinically relevant bacteria. …”
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An integrated geochemical and microbiological investigation of sulphate reduction in hypersaline pans
Published 2024“…The sulphate-reducing microbial community was quantified by competitive polymerase chain reaction. The structure of the microbial community was studied by denaturing gradient gel electrophoresis, from which bands were excised for DNA sequencing. …”
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Inactivation of critically ranked carbapenem resistant bacteria and genes in a batch atmospheric plasma reactor
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Promising antitumor therapeutics of herbal origin: exploring cytotoxic activity of glycoalkaloids and unraveling underlying mechanisms.
Published 2015“…Ki67, survivin, caspase-3, caspase-9 gene expression was evaluated through quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR). B cell lymphoma 2-associated X (Bax) protein expression was evaluated by western blot. …”
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Development of 3D multicellular liver organoids derived from human induced pluripotent stem cells to model antiretroviral therapy induced liver injury
Published 2025“…Methods: A quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was adopted to confirm stage specific gene marker and we validated the presence of multiple cell lineages using flow cytometry. …”
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CHARACTERISATION, PATHOGENICITY AND ANTIBIOTIC RESISTANCE OF MYCOPLASMAS INVOLVED IN VULVOVAGINITIS IN GOATS.
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FERMENTATION OF COCOA (Theobroma cacao L.) POD HUSK AND ITS HYDROLYSATE FOR ETHANOL PRODUCTION USING IMPROVED STARTER CULTURES
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Association of Faecalibacterium, Lachnospira, Veillonella, and Rothia with childhood wheezing
Published 2022“…Secondly, I optimized SYBR Green based real-time quantitative polymerase chain reaction (qPCR) as well as conventional PCR assays for the detection of FLVR bacteria. …”
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Targeting the CD47/Calreticulin axis in Triple Negative Breast Cancer using HA grafted Chitosan nanoparticles loaded with Doxorubicin and Polygodial
Published 2026“…Subsequently, total RNA was extracted, and quantitative real-time polymerase chain reaction (qPCR) was performed to evaluate the impact of these sublethal doses, both as monotherapy and in combination therapy, on the expression levels of CALR and CD47 in MDA-MB-231 cells. …”
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Prevalence of aetiological pathogens of vaginal discharge and male urethritis syndromes in the Tshwane region
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Efficiency of glass wool adsorption-elution technique for the recovery of enteric viruses from water
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The development of malignancies in renal allograft recipients with special emphasis on Kaposi's sarcoma
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Manufacturing GMP-grade mRNA vaccine-associated Enzymes: Bioprocess Development and Economics
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Cyclolipopeptides fengycin and iturin A as postharvest fungicides on pome fruit
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