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Allelopathic effects of aqueous extracts of olax subscorpioidea oliv. on seed germination and growth of okra (abelmoschus esculentus (l.) moench) in Ibadan, Nigeria
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DEGRADATION OF POLYAMIDE-6 BY MICROBIAL ISOLATES FROM SOLID WASTE DUMPSITES IN LAGOS STATE, NIGERIA
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ANTI-MOSQUITO PROPERTIES OF METHANOL EXTRACT OF Clerodendrum polycephalum Baker
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THE SYNTHESIS OF SOME NATURAL PRODUCTS OF MEDICINAL VALUE USING ORGANOMETALLIC COMPLEXES
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- ABSTRACT Mosquitoes are vectors of various diseases of public health importance and their control has been premised on the use of synthetic insecticides. However, these insecticides are laden with problems of high cost, environmental hazards and development of resistance in many species of mosquitoes. Alternatives to synthetic substances are the natural products. Plant species, Clerodendrum inerme, have been found to inhibit the growth of Aedes aegypti and Culex quinquefasciatus larvae. However, there is paucity of information on the anti-mosquito properties of the Nigerian species, C. polycephalum Baker. Therefore, the aim of the study was to investigate the anti-mosquito properties of the methanol extract of the leaves of C. polycephalum. Fresh leaves (1.4 kg) of C. polycephalum were harvested and brought to the laboratory. The leaves were air-dried, powdered, extracted with methanol and concentrated to dryness using rotary evaporator. The crude methanol extract was subjected to phytochemical analysis according to standard method and separated into n-hexane, dichloromethane, ethyl acetate and ethanol fractions using vacuum liquid chromatography. The most active fraction (ethanol), was subjected to column chromatography. Gas Chromatography - Mass Spectroscopy (GC-MS) was used to analyse the subfraction which was ultra-violet active. The crude methanol extract and fractions (250–8000 ppm) were tested for larvicidal activity. The methanol extract was further tested for anti-oviposition, effects on growth and development on Aedes aegypti, Anopheles gambiae and Culex quinquefasciatus mosquitoes together with corresponding positive (lambda-cyhalothrin) and negative controls (dimethylsulfoxide) using World Health Organisation procedures. Toxic effects, determined by mortality, on representative non-target organisms was evaluated on adult Biomphalaria glabrata (snail) and tadpoles of Bufo regularis. Ten each of the organisms were used per concentration. Data were analysed using descriptive statistics and student’s t-test at α0.05. Tannins, flavonoids, saponins and alkaloids were identified from the crude extract. Seventeen compounds were detected from ethanol subfraction, with quantities varying from 0.0 to 23.7%. Prominent compounds include 4-((1E)-3-Hydroxy-1-propenyl)-2-methoxyphenol (7.5%), 2-Hydroxy-5-methylbenzaldehyde (6.1%) and Ethyl iso-allocholate (23.7%). Larvicidal activities of crude methanol extract and fractions were in the order ethanol (100.0%) > methanol (96.6%) > n-hexane (7.5%) > DCM/ethyl-acetate (0.0%) at 2000ppm after 24h. Susceptibilities of mosquito larvae to methanol extract were also in the order A. aegypti > A. gambiae > C. quinquefasciatus. Mortality of the larvae was significantly different from the positive control. Anti-oviposition index increased (46.4-89.9) with extract concentrations. Morphological deformities of larvae and pupae were observed at the higher doses of 2000 ppm (A. aegypti), 5000 ppm (A. gambiae) and 7000 ppm (C. quinquefasciatus). Mosquito adult emergence inhibitions (24.0-100.0%) were recorded at 1100–5600 ppm concentration levels. The crude extract had no effect on the tadpoles at 250–1000 ppm, but the mortality of the snail at 250–2000ppm, varied from 10.0–95.0%. Clerodendrum polycephalum leaves contained biological components capable of inhibiting the survival and development of mosquitoes and could be exploited in the control of mosquitoes. 1 results 1
- Allelochemicals are being explored as environmentally friendly options in weed management and crop yield improvement as opposed to use of synthetic pesticides which have serious environmental damages. Allelopathic effects of aqueous extracts of different parts of Olax subscorpioidea on Okra seed germination in Petri-dish bioassays and okra performance in pot experiment were conducted in the Department of Crop Protection and Environmental Biology, University of Ibadan using a complete randomized design (CRD) and with treatments replicated five times. Ten okra seeds in three replicates were treated in separate petri-dishes with 2 ml water extracts of stem, leaf, fruit, bark and root of Olax subscorpioidea, and 2 ml distilled water (control) in two trials. Pot trials was carried out using okra seedlings with treated with 100 ml extracts at seven days intervals. Data were collected on number of germinated seeds daily and lengths of five randomly selected plumules and radicles at 7 days after sowing (DAS). Number of leaves, plant height, root and shoot dry weight of seedlings were also measured using standard methods. Data were analyzed by analysis of variance. Statistically significant (P<0.05) means were separated using Duncan’s Multiple Range Test. Leaf extract had the highest concentrations of phenolic, flavonoid and saponin compounds compared, while the stem extract had the least metabolite concentrations. Leaf extracts exerted highest inhibition on okra germination at 2 and 7 DAS in the first trial and at 7 DAS in the second trial compared to the bark, stem, root and fruit extracts of Olax subscorpioidea. The seedling growth and yield parameters in treatment extracts were better than control. In conclusion, Olax subscorpioidea has allelopathic potential with effects on germination and seedling growth of okra. It can be used as an alternative bioherbicide. Its production and agricultural potentials should be promoted. 1 results 1
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- Most of the maize (Zea mays L.) varieties in developing countries have low content of micronutrients including vitamin A. As a result, people who are largely dependent on cereal-based diets suffer from health challenges due to micronutrient deficiencies. Marker assisted recurrent selection (MARS), which increases the frequency of favorable alleles with advances in selection cycle, could be used to enhance the provitamin A (PVA) content of maize. This study was carried out to determine changes in levels of PVA carotenoids and genetic diversity in two maize synthetics that were subjected to two cycles of MARS. The two populations, known as HGA and HGB, and their advanced selection cycles (C1 and C2) were evaluated at Ibadan in Nigeria. Selection increased the concentrations of β-carotene, PVA and total carotenoids across cycles in HGA, while in HGB only α-carotene increased with advances in selection cycle. β-cryptoxanthine increased at C1 but decreased at C2 in HGB. The levels of β-carotene, PVA, and total carotenoids increased by 40%, 30% and 36% respectively, in HGA after two cycles of selection. α-carotene and β-cryptoxanthine content improved by 20% and 5%, respectively after two cycles of selection in HGB. MARS caused changes in genetic diversity over selection cycles. Number of effective alleles and observed heterozygosity decreased with selection cycles, while expected heterozygosity increased at C1 and decreased at C2 in HGA. In HGB, number of effective alleles, observed and expected heterozygosity increased at C1 and decreased at C2. In both populations, fixation index increased after two cycle of selections. The greatest part of the genetic variability resides within the population accounting for 86% of the total genetic variance. In general, MARS effectively improved PVA carotenoid content. However, genetic diversity in the two synthetics declined after two cycles of selection. 1 results 1
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