Development of SNAP-tag based fusion proteins as novel auristatin F-containing immunoconjugates and photoimmunotheranostics in the detection and treatment of triple-negative breast... by Mungra, Neelakshi

“…Based on the above premises, this research aims to use SNAP-tag technology as a cutting-edge site-specific conjugation method to: (1) develop a comprehensive antibody platform, consisting of single-chain antibody fragments (scFvs) genetically fused to SNAP-tag, to specifically screen and evaluate their predictive potential for chondroitin sulfate proteoglycan 4 (CSPG4), CD44 and aspartate (aspartyl/asparaginyl) β-hydroxylase (ASPH)-positive TNBC cells, (2) generate functional recombinant ADC formulations as robust delivery systems carrying the antimitotic drug monomethyl auristatin F (MMAF/AURIF), concurrently overcoming production constraints to yield therapeutically viable and homogeneous combination products, and (3) provide a fail-safe system that overcomes the lack of specificity of photodynamic therapy (PDT), by coupling scFv-SNAP-tag to the potent near-infrared (NIR) photosensitizer (PS) IRDye700DX (IR700) and to demonstrate its selective dose-dependent cytotoxic activities in vitro. …”
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Investigating the host range of Neoromicia capensis coronavirus in vitro by Kotze, Andrea

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Synthesis and biological evaluation of auristatin-F recombinant antibody-drug conjugates for cancer therapy by Huysamen, Allan Martin

“…This study serves as a contribution to the emerging field of antibody-drug conjugate (ADC) therapeutics, in which novel ADCs were generated and biologically evaluated for therapeutic potential and specificity towards cancer cells. …”
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Repurposing quinoline-based (metallo) drug leads for the treatment of Leishmania major-induced cutaneous leishmaniasis by Murwira, Takudzwa Emmanuel

“…The cytotoxicity of the compounds was also assessed against the murine RAW 264.7 macrophage cell line, and all four compounds were observed to be more cytotoxic than amphotericin B (CC50 < 50 μg/ml). …”
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Improvement of foot-and-mouth disease virus vaccines and diagnostics through structural design

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Survival of oxidative stress-adapted Bifidobacterium spp. in yoghurt

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Developing quantitative approaches to determine microbial colonisation and activity in mineral bioleaching and characterisation of acid rock drainage by Makaula, Didi Xhanti

“…Correlation was shown between number of cells detached from the mineral surface and the heat generated, with a constant heat output per cell observed until day 15 of operation. …”
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Proteomic assessment of potential in vitro hepatotoxicity models

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Development and evaluation of immunogens for a yellow tulp (Moraea pallida) vaccine

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Mixtures effects of nanoscale and microscale contaminants to Bacillus subtilis in natural water systems

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Cellular radiotoxicity of iodine-123 by Smit, B. S.

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Development Of Recombinant Immunotherapeutics for Triple-Negative Breast Cancer (TNBC) by Ngwenya, Takunda

“…This study showed potential for clinical application of the generated rITs' for TNBC immunodiagnostic use and the ability of ETA and dETA toxins to specifically kill TNBC antigenpositive tumour cells indicating targeted therapeutic potential. …”
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Systems biology of organellar carbon metabolism in Eucalyptus xylogenesis

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Functional characterisation of African horse sickness virus non-structural protein NS3 by reverse genetics.

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Power management of a 1kW HTPEMFC based CHP system by Reddy, Direshni

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Water-gas shift conversion in microchannel reactors using noble metal catalysts by Maduna, Kubefu Albert

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The transciptomic landscape of HIV-TB by Deffur, Armin

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An investigation of the importance of the ATM protein in the endothelium and its role in the signalling pathways of NO production by Collop, Natalie Chantel

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The role of Cysteinyl leukotriene type 1 receptor (CysLTR1) during Listeria monocytogenes infection in mice by Poswayo, Sibongiseni Kwakho Luntukazi

“…Production of cytokines and eicosanoids by antigen presenting cells activates the adaptive immunity. Eicosanoids (epoxyeicosatreinoic acids, prostanoids and leukotrienes) are generated from metabolites of 20-carbon chained polyunsaturated fatty acids and arachidonic acid. …”
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The Influence of HIV-1 Subtype C LTR Genotype on Latency Potential by Doolabh, Deelan Sudhir

“…We observed consistently greater proportions of latently infected cells than actively infected cells. In Jurkat E6-1 cells, the median latent:active infection ratio was 1.97 (range 0.86-2.83; three experiments). …”
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