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Kin Endogamy and the Blood Taint in Ancient Egypt and Nigeria
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CATION CONTENT AND FLUXES IN RED CELLS OF NORMAL AND HYPERTENSIVE NIGERIANS
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MOLECULAR PREDICTIVE BIOMARKERS FOR ORAL SQUAMOUS CELL CARCINOMA IN HUMANS IN IBADAN, NIGERIA
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Page will reload when a filter is selected or excluded.- Conservation 3 results 3
- Biodiversity conservation 2 results 2
- Biodiversity health 2 results 2
- Childhood thyroid carcinoma 2 results 2
- Cowpea radio-sensitivity 2 results 2
- Funisciurus anerythrus 2 results 2
- Gamma irradiated cowpea seed 2 results 2
- Genetic diversity 2 results 2
- Genetic variations 2 results 2
- Heliosciurus gambianus 2 results 2
- Immune genes 2 results 2
- Mutant phenotypes 2 results 2
- Pollen mutagenesis 2 results 2
- Ultra-violet irradiated pollen 2 results 2
- A cemetery, because of its presumed sanctity, is less disturbed from other land use activities in the urban areas and thus serves in many cases as the last remnant for greenery and conservation of trees in many large cities. However, there is little information on the population, diversity and management of trees in cemeteries located in the Ibadan metropolis. Therefore, this study was carried out to determine the potentials of cemeteries as urban tree conservation areas. Eight cemeteries under different management within the metropolis were sampled and their tree species composition, density and population determined. Their land areas were also determined and the trees, classified based on their origin and uses. The size of the cemeteries ranged from 0.03 - 0.48 km2. There were 48 tree species from 22 different families represented in the eight cemeteries, with 28 of these species indigenous to the lowland rainforest ecosystem. Fabaceae family had the highest species representation. Cemeteries under government and institutional management had a larger representation of trees than the private and religious ones, with the highest number of trees (399) found in the Ibadan South East Cemetery, Orita-Aperin which had a tree density of 0.014 m-2. The University of Ibadan cemetery recorded the highest species composition (28) with a tree density of 0.03 m-2. Albizia zygia had the highest number of identified uses (15), while shade and shelter were the most common attributes of trees in the cemeteries. They could assist in the preservation of tree genetic diversity and serve as botanic refuge for many threatened species. Therefore, management of modern city cemeteries require enlightenment on the conservation role such facilities could play in urban areas. 1 results 1
- A nationwide equine influenza virus surveillance was carried out between January 1989 and January 1995 during which nasopharyngeal swabs, tracheal washes and respiratory tract tissue homogenates were obtained from symptomatic and asymptomatic equine species in various parts of Nigeria for virus isolation and characterisation. Three equine influenza viruses were independently isolated in 10-11 day old embryonated hen eggs and Madin Darby canine kidney monolayer cells with TPCK-trypsin incorporated into the culture medium. The virus isolates were characterized antigenically, immunogenically and genetically which made them the first equine influenza viruses isolated and characterized in tropical Africa. Antigenic analyses with mono-specific antisera raised against a number of respiratory disease viruses including Paramyxoviruses, Adenoviruses, Herpes viruses, Alphavirus, Rhinoviruses, Equine arteritis viruses, African horse sickness viruses and Influenza viruses showed that the three virus isolates were influenza A viruses while haemagglutination and neuraminidase inhibition assays using influenza virus specific Mabs and polyclonal antisera showed the three viruses to be antigenically H3N8, members of equine-2 subtype of influenza A viruses. The three isolates were respectively named A/Equine/Ibadan/4/91, A/Equine/Ibadan/6/91 and A/Equine/Ibadan/9/91 in accordance with the recommendations of the World Health Organisation and the International Committee on Virus Taxonomy. Their antigenic cross-reactivity with panels of Mabs and polyclonal antisera indicated that the three viruses were antigenically divergent although they were all H3N8 viruses and were representatives of a homogenous population. They were reactive with Mabs and antisera directed against H3 equine viruses isolated in 1963, 1976, 1979, 1981 and 1986. This broad reactivity suggested that these isolates were similar to but not still different from those isolated in Europe and USA between 1963 and 1987. Virus protein synthesis and cleavability of the HA polypeptide in tissue culture were investigated by pulse-chase experiments. The results showed some heterogeneity in the non-glycosylated polypeptides particularly those of the ribonucleoprotein (RNP) complex while the HA glycoproteins of the three viruses were not cleaved in any of the cell types used in contrast to equine H7 and pathogenic avian H5 and H7 HAs. The molecular weights of the polypeptides were within the range previously determined for influenza A viruses. The heterogeneity of the RNP complex and antigenic divergence of the viruses’ HAs were confirmed by sequence analysis carried out in molecular studies. Virus infectivity was investigated by plaque assay using chicken embryo fibroblast (CEF) monolayer cells and virus titration in embryonated hen eggs. The results showed that the viruses were infectious with plaguing efficiency being comparable to efficiency of virus infectivity in embryonated hen eggs. Infectivity, antigenicity and immunogenicity of the three viruses were confirmed in-vitro using peripheral blood lymphocytes in lymphoproliferation assays and in-vivo in an equine model in which the viruses induced haemagglutination inhibiting and protective neutralising antibodies following experimental infections. The proliferating cells were also characterised and the immunoglobulin isotypes produced were determined. Molecular characterisation and genetic analyses of the three viruses were accomplished to determine the origin of the genes encoding the virus non-glycosylated polypeptides as well as those of the surface HA glycoprotein. Reverse transcription (RT) results showed the eight RNA segments of the three isolates and confirmed them as influenza A virus RNAs. No subgenomic RNAs or defective interfering particles were observed in the RNA transcripts. Nucleotide sequence analyses were carried out using three sequencing strategies of cDNA, vRNA and plasmid DNA sequencing with the dideoxy chain termination procedure. Partial nucleotide sequences were obtained from cDNAs, vRNAs and plasmid DNA clones of RNA segments 1, 2, 3, 5, 7 and 8 with the method of multiplex RT/PCR and cycle sequencing using radiolabelled segment specific oigonucleotides of 18, 24 or 25 mers. Complete nucleotide sequences of segment 4 (HA genes) were also determined on the same PCR products (cDNA) and vRNA using end-labelled oligonucleotides of both plus and minus sense. The partial nucleotide sequence data were analysed using a programme for "best-local-homology- rapid-search" on a digital array processor while the complete HA nucleotide and deduced amino acid sequence analyses were carried out using the University of Wisconsin Genetics Computer Group (GCG) package of programmes. Phytogenetic analysis was done with the distances, neighbour joining and DNAPARS of the PHYLIP package. Analyses of the viruses’ gene sequences confirmed that their genomes were similar to each other and to those of other H3N8 influenza viruses isolated from equines and also revealed the origin, evolution and genetic relatedness of the genes. Comparison of the partial cDNA sequences with virus DNA sequences in the database (EMBL sequence library) showed that for segments 1, 2, 5, and 7, the closest related sequences were from equine H3 viruses isolated in 1986 in USA (Tennesse/5/86 for segments 2, 5 and 7, Kentucky/2/86 for segments 1 and 5. Segment 5 was equally related to both viruses). The nucleotide sequence for segment 3 was most closely related to an equine-1 virus isolated in U.K. in 1973 (London/1416/73, H7N7) probably due to genetic exchange while segment 8 sequence was most closely related to an equine H3N8 virus isolated in U.K. in 1976 (Newmarket/76). The complete nucleotide and deduced amino acid sequences as well as phytogenetic analysis of the HA genes (RNA segment 4) showed a closer relationship albeit with nucleotide and amino acid substitutions between the three Ibadan viruses and those that were isolated in Europe in 1989 and 1991, the prototypic European strain, Suffolk/89 and Arundel/12369/91 isolated in U.K., Taby/91 isolated in Sweden, Hong Kong/92 isolated in the Far East in 1992 and Laplata/1/93 isolated in South America. These findings group the Ibadan viruses with those predominating and contemporarily causing disease in the Western Hemisphere rather than with viruses previously isolated from the north and south of African continent. Variation was observed in the nucleotide sequences of the Ibadan viruses HA genes. Some of the base changes resulted in amino acid changes which mapped to antigenic sites or within signal sequence in the HA1 domain as a result of a process of antigenic or genetic drift. The Ibadan viruses also showed some variation from the prototypic European virus (Suffolk/89) and these base changes also resulted in amino acid changes resulting in antigenic drift. Phytogenetic analysis showed the evolutionary lineages in equine H3 viruses isolated since 1963 along two paths one of which included the Ibadan viruses as well as viruses isolated in Europe between 1989 and 1991 and the Far East in 1992 and S. America in 1993 which form the 1989/93 cluster while the other lineage included viruses isolated in South America in 1987 and 1988 (Brazil/87, Laplata/88) and in the Far East in 1971 (Tokyo/3/71) all of which are very close to the original prototype equine-2 virus (Miami/63). These results demonstrate a faster evolutionary rate for recent equine H3 HA genes away from the original prototype virus. Overall, the results of these studies have (i) confirmed the occurrence of equine-2 H3N8 influenza viruses of distinct lineages in Nigerian equine populations in a tropical environment (ii) indicated antigenic drift among equine H3N8 viruses as earlier reported and confirmed that drift strains can co-circulate in equine populations, (iii) showed the origin, evolution and genetic relatedness of the viruses genes as well as their biological characteristics, (iv) provided the hitherto unavailable information on the status of equine influenza virus in this part of the world and (v) served to re-emphasise the potential of influenza virus for rapid global spread and the need for better control strategies. 1 results 1
- Aberrant CpG methylation 1 results 1
- Africa 1 results 1
- Allozyme 1 results 1
- Attempts to determine interspecific differences in patterns of protein variation in edible frog species have been relatively few. Therefore, this study determined the genetic variation in populations of Hoplobatrachus occipitalis and Xenopus muelleri in two locations [University of Ibadan (UI) and Igbo Oloyin area (IO)] in Ibadan, Nigeria, using five allozyme loci. Eighty (80) edible frogs comprising twenty (20) live samples each of H. occipitalis and X. muelleri species from IO and UI were collected. 1ml of blood was drawn per sample via cardiac venipuncture. Plasma fractions were obtained and subjected to Cellulose Acetate Electrophoresis to determine the genetic variations at Haemoglobin (HB), Carbonic anhydrase (CA), Transferrin (TF), Albumin (AL) and Esterase (EST) loci. Test for Hardy–Weinberg Equilibrium (HWE; ), Heterozygosity [observed (Ho) and expected (He)] and Genetic differentiation (FST) were estimated. Significant deviations from HWE were observed at several loci in all the populations. Ho was higher (than He) for both populations of H. occipitalis but lower for both populations of X. muelleri. FST was 0.0559 and 0.0264 for the populations of H. occipitalis and X. muelleri, respectively. There is an indication of evolutionary forces in operation in the IO populations of H. occipitalis and X. muelleri. 1 results 1
- Background Thyroid carcinoma is a common endocrine malignancy. It is however rare in childhood and often occurs as a result of radiation exposure or inherited genetic mutations. Most childhood thyroid carcinomas are well differentiated. There are very few epidemiological studies of this disease in Nigeria and our study aimed to determine the frequency in a subset of our population. The study aimed to determine the prevalence and histopathological characteristics of childhood thyroid carcinoma in our environment. Methods This was a retrospective review of histopathologically diagnosed thyroid carcinoma in children less than 18 years of age in the Department of Pathology, University College Hospital, Ibadan over a 40-year period. Histopathological diagnosis, age, sex and other relevant clinical information were extracted from the hospital records and surgical pathology records of the department. All cases of patients under18 years old had their slides re-examined and reclassified by two pathologists according to the World Health Organization histopathological classification of thyroid tumours. Results There were 25 cases of thyroid carcinomas seen in children within the study period. Papillary thyroid carcinoma was the most common, accounting for 80% of the cases. Follicular carcinoma accounted for 12%, and medullary and anaplastic carcinoma accounted for 4% each. The mean age at presentation was 13 years. There was a female preponderance with females accounting for 60% of cases. Tumours with distant metastasis made up 20% of the cases. Conclusion Childhood thyroid carcinomas are rare in our environment, with a minority of cases presenting with metastases. 1 results 1
- Background Thyroid carcinoma is a common endocrine malignancy. It is, however, rare in childhood and often occurs as a result of radiation exposure or inherited genetic mutations. Most childhood thyroid carcinomas are well differentiated. There are very few epidemiological studies of this disease in Nigeria, and our study aimed to determine the frequency in a subset of our population. The study aimed to determine the prevalence and histopathological characteristics of childhood thyroid carcinoma in our environment. Methods This was a retrospective review of histopathologically diagnosed thyroid carcinoma in children less than 18 years of age in the Department of Pathology, University College Hospital, Ibadan, over a 40-year period. Histopathological diagnosis, age, sex, and other relevant clinical information were extracted from the hospital records and surgical pathology records of the department. All cases of patients under 18 years old had their slides re-examined and reclassified by two pathologists according to the World Health Organization histopathological classification of thyroid tumours. Results There were 25 cases of thyroid carcinomas seen in children within the study period. Papillary thyroid carcinoma was the most common, accounting for 80% of the cases. Follicular carcinoma accounted for 12%, and medullary and anaplastic carcinoma accounted for 4% each. The mean age at presentation was 13 years. There was a female preponderance, with females accounting for 60% of cases. Tumours with distant metastasis made up 20% of the cases. Conclusion Childhood thyroid carcinomas are rare in our environment, with a minority of cases presenting with metastases. 1 results 1
- Background: The UCH/Smile Train partnership, which offers free cleft surgeries to patients provides succor. Objective: The objective of the study was to determine the state distribution of new patients presenting with cleft lip and palate, to The UCH. Method: A retrospective review of all new cleft patients presenting to The UCH between January 2012 and June 2015. The data obtained were their local government area of residence, age of the patients, gender of the patients and the type of cleft. Descriptive statistics was used to analyze the distribution of patients seen while Chi square test was used to analyze the influence of gender and laterality on the type of cleft. Results .'Sixty-seven eligible patients were seen within the study period. Majority (83.6%) of patients seen were from 14 of the 33 Local Government Areas (LGA’s) in Oyo state. Patients were seen from LGA’s in proximity to UCH. A few (16.4%) of the patients came from outside the state. Iwajowa, the LGA with the least number of patients (1.8%) was furthest from UCH. Left sided clefts were significantly more than bilateral or right-sided clefts (p=0.001). Most of the patients from Oluyole LGA had CP while no patient with CP was seen from Lagelu and Akinyele LGA’s. Conclusion: There is the need to intensify cleft awareness programs. Further studies into the health habits, cultural beliefs and genetic profile of communities may explain some regional distribution of cleft types seen. 1 results 1
- Biodiversity 1 results 1
- Cataract 1 results 1
- Cleft distribution 1 results 1
- Cleft lip 1 results 1
- Cleft palate 1 results 1
- Computer Science & IT 1 results 1
- Cowpea, Vigna unguiculata, is an important human and livestock protein source in Nigeria, but its production is constrained by ravages of pests and climate change. Conventional breeding efforts used to fortify it against these constraints had resulted into its narrow genetic base. In order to overcome this challenge, other mutation procedures such as physical and chemical mutagens could be used. Nevertheless, information on gamma and ultra-violet (UV) irradiated cowpea seeds and pollen has not been adequately documented. Therefore, genetic analysis of cowpea mutants from gamma and UV irradiated seeds and pollen, respectively was investigated. Cowpea accessions seeds: IB, IB-Y1, IB-CR and IB-BPC from the University of Ibadan and IT86D-719, IT86D-1010, IT89KD-347-57 and IT90K-284-2 from International Institute of Tropical Agriculture were irradiated at 100, 200, 300, 400 and 500 Gy doses at the rate of 202 Gy/min using 60Co gamma. Pollen were irradiated for 60, 120, 180, 240, 300 and 360 minutes at 30,000µWs/cm2 UV prior to hand self-pollination using standard procedures. Radio-sensitivity of irradiated accessions were determined using seed germination (SG), seedling survival (SV), lethal dosage 50% (LD50) for SG and SV, primary leaf area (PLA) and seed set (SS) at M1 and M2 generations. The M1 of gamma irradiation (GI) and M2 of UV irradiation (UVI) treatments were advanced to M2GI and M3UV for phenotyping on field and their genetic stability confirmed at M3GI and M4UV, respectively. Genetic diversity of all mutants was determined using microsatellites. Ribulose-bisphosphate carboxylase primers were used for sequence analysis and classification of the mutants. Inheritance pattern was evaluated at M5 of gamma induced mutants (GIM) for erect-tall (ER), yellow flush (YF), four-primary leaf (FP), crinkled leaf, lettuce leaf (LL), twisted-pale leaf (TP) and burnt leaf (BL) traits. Data were analysed using descriptive statistics and Chi-square at α0.05. The M1 generation of IT90K-284-2 had 74.0% SG, while each of IB, IB-Y-1, IB-CR and IB-BPC had 20.0% SG at 500 Gy of GI. The IB, IB-Y-1, IB-CR and IB-BPC had 0.0% SV each, while 50.0% was observed in IT86D-1010 and IT90K-284-2 at 400 and 500 Gy, respectively. The LD50 for SG and SV were lowest (326 and 149 Gy, respectively) in IB-Y-1 and highest (1053 and 620 Gy, respectively) in IT90K-284-2. The PLA of M1 ranged from 2.17±0.26cm2 to 5.98±0.85cm2. Low GI (100 Gy) and UVI (60min) increased SS of M1 plants. Mutant phenotypes and frequencies varied across the cowpea accessions and did not correspond to GI treatments. Ten GIM were stable at M3, whereas all UV induced mutants reverted to normal at M4. Polymorphic information content (0.51) obtained from microsatellites showed wide genetic diversity among the mutants and parental lines. The main mutant classes were insertion-deletions and point mutations. Inheritance of ER, TP, YF, FP and BN followed monogenic recessive pattern. Genetic interaction of crinkled and TP in homozygous recessive (crl crl tp tp) conditioned LL phenotype. Radio-sensitivity of cowpea to gamma irradiation varied among the accessions. Ultra-violet radiation was less potent for cowpea pollen mutagenesis and might not be effective for mutation breeding. 1 results 1
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