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HYGIENE FACTORS INFLUENCING OPPORTUNISTIC INFECTIONS AMONG PEOPLE LIVING WITH HUMAN IMMUNODEFICIENCY VIRUS AND ACQUIRED IMMUNE DEFICIENCY SYNDROME IN ABUJA, NIGERIA
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CIRCULATING TUMOUR ASSOCIATED ANTIGENS AS AID TO EARLY DIAGNQSIS OF CARCINOMA OF CERVIX
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Page will reload when a filter is selected or excluded.- Acquired Immune Deficiency Syndrome (AIDS) is one of the most devastating global health pandemics. The immune suppression of infected persons predisposes them to opportunistic infections resulting in high morbidity and mortality. Factors influencing HIV/AIDS in Nigeria are known; however, the influences of physical environmental factors on People Living with HIV and AIDS (PLWHAs) in Nigeria have not been fully explored. This study was therefore designed to investigate the association between these factors and the occurrence of Opportunistic Infections (OIs) among PLWHAs in Abuja. A case control study was conducted. PLWHAs in Abuja Municipal Area Council (AMAC) were randomly selected from the six area councils in FCT. Two health facilities were randomly selected by balloting from the nine that provides special services to PLWHAs in AMAC. Systematic random sampling was used to recruit 66 cases and 68 controls from the facilities. Cases were defined as HIV positive clients with OIs while controls were defined as confirmed HIV positive clients not manifesting OIs. Opportunistic Infections considered were Tuberculosis (TB), persistent diarrhea, oral thrush and herpes. The controls were matched by sex, age and residential area. Respondents’ demographic characteristics, level of knowledge and hygiene practices were assessed using a validated questionnaire. The level of knowledge of Environmental Influence on Health (EIH) was determined using 40 point scale. Observational checklist was used to assess the prevailing environmental conditions in households. Bacteriological examination of drinking water sources of 25.0% of cases and controls was conducted using standard methods as described by the American Public Health Association. Descriptive statistics, Chi-square and t-test were used for data analysis. Respondents’ mean age was 35.9 ± 6.7years for cases and 34.4 ± 7.0years for controls. About 48.6% and 51.4% of cases and controls respectively were females. The OIs manifested by cases were TB (56.6%), oral thrush (77.4%), persistent diarrhea (69.8%) and herpes (11.3%). The mean knowledge scores on EIH of cases and controls were 31.7 ± 8.9 and 29.0± 13.1 with no significant different. Majority of the cases (75.8%) and control (70.6%) washed their hands with soap after toilet use. Also 66.7% of cases and controls (68.7%) used water closets for excreta disposal. About twenty four percent of cases as against nine percent of controls obtained drinking water from contaminated sources (OR: 3.25; 95%CI: 1.09 – 10.14). Bacteriological analysis of client drinking water sources indicated that 76.0% of samples for cases and 64.3% for controls contained total coliform, while E.coli was detected in 21.4%, and 15.4% of samples for cases and controls respectively. The occurrence of water borne opportunistic infections among the cases may be attributable to contaminated drinking water sources. Provision of water especially potable water supply and continuing health education on hygiene practices are highly advocated. 1 results 1
- Earlier studies by this author in 1976 provided evidence of an association between an infective, sexually transmitted agent, the Herpes Simplex Virus Type-2 (HSV—2) and human carcinoma of the cervix, irrespective of geographic location or ethnic origin of the individuals. Similarly, HSV-2 related antigens were demonstrated by the indirect immuno-fluorescent technique in desquamated cells of carcinoma of the cervix tissues from tumour bearing patients. To highlight the Potentials of the detection of tumour antigens in tumour-faearing patients as aid to early diagnosis of the cancer, Isolation and purification of the Tumour Associated Antigens (TAA) of human cancer of the cervix were attempted by immunological and physicochemical procedures in this study. Two antigen preparations, one the Soluble Antigens (SA), and the other, the Membrane Bound Antigens (MBA) were prepared from a pool of cancer of the cervix (CaCx) tissues. The soluble antigens were extracted by homogenization in Earle’s Balanced Salt Solution (EBSS) containing antibiotics, and the membrane-bound antigens were solublised by 3-molar potassiurn Chloride (3 Molar KCl). For isolation and purification of TAA, procedures for the purification of proteins were explored. These include physicochemical stepwise ammonium sulphate fractionation, sephadex ion exchange chromatography, sephacryl S-200 gel filtration, and affinity chromatography to eliminate the normal tissue components of the antigen fractions. Hyperimmune rabbit sera were then prepared against the partially purified TAA. Immunodiffusion studies, employing hyperimmune rabbit sera prepared against the partially purified TAA were used to demonstrate tumour associated antigens in the various cancer antigen preparations, and Circulating Tumour Associated Antigens (C-TAA) in the sera of tumour- bearing patients. The TAA in the cancer tissues showed lines of identity with the C-TAA in patients sera. Techniques involving adsorption by immuno-precipitation-in-gel with Normal Cervix (NCx) tissue antigen preparations, and pooled Normal Human Sera (NHuS) were utilized for the removal of antibodies to NCx and NHuS in the rabbit sera, after which there were no reactions with NCx indicating the specificity of TAA for CaCx. A comparison of the soluble and 3 Molar KCl extracts of the cancer antigens by immuno-precipitation reaction, using adsorbed hyperimmune rabbit sera prepared against the partially purified CaCx TAA, demonstrated three TAA (TAA-1, close to the central antisera well, TAA-2, intermediate and TAA-3, close to and curving towards the peripheral antigen well) in the soluble antigen preparation. Only one TAA (probably identical to the TAA-3 of the soluble antigen) was demonstrated in the 3 Molar KCl extract. Using the rabbit antisera against the partially purified CaCx TAA, adsorbed with NCx and NHuS, results of coded sera showed that immunodiffusion reaction was able to detect circulating TAA in 75.0 per cent of patients with cancer as compared with 5.6% in women with benign gynecological diseases, 1.4% in pregnant women and 0.0% in healthy control women. The result indicates that the test has great potential for immunodiagnosis of cancer of cervix. Although the sensitivity of the test method was low, the specificity was high, and could provide a means of early diagnosis of neoplastic changes in the cervix. The detection of Circulating-TAA as tumour markers in sera of patients, may someday become routine, and thus make earlier diagnosis of cancer possible. Indications are that immuno-diagnostic procedures can be designed in ways that are reproducible, simple and reliable. Such serological tests would extend our present ability for detection and monitoring of malignancies. 1 results 1
- HIV/AIDS, 1 results 1
- Hygiene practices 1 results 1
- Opportunistic infection 1 results 1
- People Living with HIV and AIDS (PLWHA) 1 results 1