Genetic diversity of subtype C HIV-1 env variants in peripheral blood mononuclear cell (PBMC) DNA from infected mother-child pairs : a comparison of Heteroduplex Mobility Assay (HM... by Loubser, A S

Get full text

Development of SNAP-tag-based fusion proteins targeting HIV-1 viral reservoirs by Cingo, Siphelele Sanele

“…Molecular cloning of designed open reading frames (ORFs) was performed into appropriate bacterial expression plasmid vectors. Plasmid vectors confirmed to contain the required ORFs by Sanger sequencing were transformed into E. coli BL21-DE3. …”
Get full text

Developing novel SNAP tag-based antibody fusion proteins directionally conjugated to fluorophores for immunophenotyping of acute myeloid leukemia by Bvudzijena, Tatenda Lovemore

“…The variable gene sequences encoding for scFv antibody fragments were obtained from publicly available resources (patents, journals, and antibody databases) and were fused to SNAP by in-silico expression plasmid design. The SNAP fusion protein encoding open reading frames (ORFs) were then cloned into the pCB plasmid vector backbone, containing all the unique features for protein expression in mammalian systems. …”
Get full text

HPV pseudovirion production in plants by Kennedy, Paul

Get full text

Molecular characterization of Listeria spp. isolated from cattle farms, abattoirs, and beef products in Mpumalanga and North-West Provinces, South Africa

Get full text

Studies on regulation of the plantaricin 423 gene by Cohen, Francisca

Get full text

Colistin resistance in gram-negative pathogens in the Western Cape, South Africa by Snyman, Yolandi

Get full text

Identification and characterization of a novel thermohalophilic esterase from the Red Sea: Atlantis II brine pool by Mohamed, Yasmine Mustafa

“…Sequencing of positive clones identified a novel lipolytic enzyme (EstATII), which was then subcloned from the original fosmid into a high copy number plasmid to allow simple overexpression and characterization of the enzyme. …”
Get full text
Get full text

An exploratory investigation into the physicochemical, antioxidant and cellular effects of a selection of honey samples from the Southern African region

Get full text
Get full text

Rhamnose metabolism and metronidazole resistance in B. thetaiotaomicron VPI-5482 by Patel, Ekta H

Get full text

The development of transgenic plants resistant to cucumber mosaic virus and tobacco necrosis virus by Hackland, Andrew F

Get full text

The utility of a real-time PCR to detect Leptospira in a routine diagnostic setting by Chanda, Raphael

Get full text

Reactor design for the hydroxylation of n-octane using a cyp153a6-based biocatalytic system expressed in Escherichia coli by Meissner, Murray Peter

Get full text

Development and validation of an Ehrlichia canis real-time PCR assay

Get full text

Characterization of nisin F and its role in the control of respiratory tract and skin infections by De Kwaadsteniet, Michele

Get full text

Genomics of West Nile viruses from South Africa

Get full text

Characterisation of two aphid picorna-like viruses by Williamson, Carolyn

Get full text

Molecular analysis of two cellulase genes from Ruminococcus flavefaciens FD-1 and their transcriptional regulation by Wang, Wenyen

Get full text

Expression of the VP1 antigen from foot-and-mouth disease virus in a bacterial and plant-based expression system

Get full text
Get full text

Characterization of tolerance to bacterial wilt in the model plant Arabidopsis

Get full text