Investigation of particulate Bluetongue virus vaccines made in plants by Gwynn, Abigail

“…The 60kDa coupled complex was detected on Western blots and Coomassie gels with an estimated protein concentration of approximately 0.03ug/uL and a coupling efficiency of 44%. Finally, since there was insufficient coupled protein product for immunisation doses, only the immunogenicity of the plant produced chimeric BTV1/8 VLPs compared with the BTV8 VLPs were tested. …”
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The effect of extrusion with molasses and addition of chitosan or tannins on the rumen undegradable protein fraction of plant protein sources by Jordaan, Leanne

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