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Fabrication and characterization of antibacterial herbal drug-loaded polylactic acid/cellulose acetate composite nanofiberous for wound dressing application.
Polylactic acid (PLA) is a versatile biopolymer that is widely used as a biomaterial. However, one of the major issues which limits its further application in tissue engineering purposes is its hydrophobic nature and poor cellular interaction. Modification of PLA properties can be achieved by polyme...
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2016
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| _version_ | 1867613408301416448 |
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| access_status_str | Open Access |
| author | Khalifa, Salma Fouad |
| author_browse | Khalifa, Salma Fouad |
| author_facet | Khalifa, Salma Fouad |
| author_sort | Khalifa, Salma Fouad |
| collection | Thesis |
| dc_rights_str_mv | The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. |
| description | Polylactic acid (PLA) is a versatile biopolymer that is widely used as a biomaterial. However, one of the major issues which limits its further application in tissue engineering purposes is its hydrophobic nature and poor cellular interaction. Modification of PLA properties can be achieved by polymer blending techniques. Polymer blending is a simple yet attractive method to combine and optimize polymeric physical properties of interest. In this study, an antibacterial electrospun nanofibrous scaffolds, with diameters around 400–1000 nm, were prepared by physical blending PLA with a hydrophylic biopolymer, cellulose acetate (CA). In this stage, PLA was used as the main polymer, blended with CA, at two main ratios (9:1 and 7:3 w/w), to achieve desirable properties such as better hydrophilicity, excellent cell attachment and proliferation. For preventing common clinical infections, an antimicrobial agent, Thymoquinone, TQ was incorporated into the electrospun fibers. TQ is the active ingredient of Nigella sativa and it is well known for its antibacterial properties. The potentiality of the prepared scaffolds, regarding being used as an interactive wound dressing, has been investigated including, swelling behavior, WVP and porosity. The release profile of TQ from the prepared scaffolds was also examined at the physiological pH (7.4) and temperature (37 οC). The antimicrobial efficiency of the prepared scaffolds against gram negative and gram positive bacteria were determined by the agar diffusion assay. The interaction between fibroblasts and the TQ-loaded PLA: CA scaffolds such as viability, proliferation, and attachment were characterized. TQ-loaded PLA: CA scaffolds showed burst TQ release after 24 h, compared with medicated PLA scaffolds, followed by a sustained release rate for 9 successive days. The results also indicated that medicated PLA: CA nanocomposite scaffolds showed a significant antibacterial activity against both gram positive and gram negative bacteria. Furthermore, the prepared scaffolds enhanced cell viability, attachment and proliferation, as compared to medicated PLA nanofibers. The presence of CA in the nanofiberous scaffolds improved its hydrophilicity, bioactivity, and water uptake capacity. Furthermore, it created a moist environment for the wound, which can accelerate wound recovery. A preliminary in vivo study performed on normal full thickness mice skin wound models demonstrated that TQ-loaded PLA: CA (7:3) scaffolds significantly accelerated the wound healing process by promoting angiogenesis, increasing re-epithelialization and controlling granulation tissue formation. Our results suggest that TQ-loaded PLA: CA nanocomposite mat could be an ideal biomaterial for wound dressing applications. |
| format | Thesis |
| id | oai:fount.aucegypt.edu:etds-1203 |
| institution | American University in Cairo (Egypt) |
| last_indexed | 2026-06-10T12:35:39.635Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from AUC Knowledge Fountain — bepress |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | AUC Knowledge Fountain |
| publisherStr | AUC Knowledge Fountain |
| record_format | dspace |
| source_str | AUC Knowledge Fountain — bepress |
| spelling | oai:fount.aucegypt.edu:etds-1203 Fabrication and characterization of antibacterial herbal drug-loaded polylactic acid/cellulose acetate composite nanofiberous for wound dressing application. Khalifa, Salma Fouad Polylactic acid (PLA) is a versatile biopolymer that is widely used as a biomaterial. However, one of the major issues which limits its further application in tissue engineering purposes is its hydrophobic nature and poor cellular interaction. Modification of PLA properties can be achieved by polymer blending techniques. Polymer blending is a simple yet attractive method to combine and optimize polymeric physical properties of interest. In this study, an antibacterial electrospun nanofibrous scaffolds, with diameters around 400–1000 nm, were prepared by physical blending PLA with a hydrophylic biopolymer, cellulose acetate (CA). In this stage, PLA was used as the main polymer, blended with CA, at two main ratios (9:1 and 7:3 w/w), to achieve desirable properties such as better hydrophilicity, excellent cell attachment and proliferation. For preventing common clinical infections, an antimicrobial agent, Thymoquinone, TQ was incorporated into the electrospun fibers. TQ is the active ingredient of Nigella sativa and it is well known for its antibacterial properties. The potentiality of the prepared scaffolds, regarding being used as an interactive wound dressing, has been investigated including, swelling behavior, WVP and porosity. The release profile of TQ from the prepared scaffolds was also examined at the physiological pH (7.4) and temperature (37 οC). The antimicrobial efficiency of the prepared scaffolds against gram negative and gram positive bacteria were determined by the agar diffusion assay. The interaction between fibroblasts and the TQ-loaded PLA: CA scaffolds such as viability, proliferation, and attachment were characterized. TQ-loaded PLA: CA scaffolds showed burst TQ release after 24 h, compared with medicated PLA scaffolds, followed by a sustained release rate for 9 successive days. The results also indicated that medicated PLA: CA nanocomposite scaffolds showed a significant antibacterial activity against both gram positive and gram negative bacteria. Furthermore, the prepared scaffolds enhanced cell viability, attachment and proliferation, as compared to medicated PLA nanofibers. The presence of CA in the nanofiberous scaffolds improved its hydrophilicity, bioactivity, and water uptake capacity. Furthermore, it created a moist environment for the wound, which can accelerate wound recovery. A preliminary in vivo study performed on normal full thickness mice skin wound models demonstrated that TQ-loaded PLA: CA (7:3) scaffolds significantly accelerated the wound healing process by promoting angiogenesis, increasing re-epithelialization and controlling granulation tissue formation. Our results suggest that TQ-loaded PLA: CA nanocomposite mat could be an ideal biomaterial for wound dressing applications. 2016-02-01T08:00:00Z thesis application/pdf https://fount.aucegypt.edu/etds/204 https://fount.aucegypt.edu/context/etds/article/1203/viewcontent/First_20draft_20animal_20study.pdf The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. Theses and Dissertations AUC Knowledge Fountain Wound dressing Polymeric nanofibers |
| spellingShingle | Wound dressing Polymeric nanofibers Khalifa, Salma Fouad Fabrication and characterization of antibacterial herbal drug-loaded polylactic acid/cellulose acetate composite nanofiberous for wound dressing application. |
| title | Fabrication and characterization of antibacterial herbal drug-loaded polylactic acid/cellulose acetate composite nanofiberous for wound dressing application. |
| title_full | Fabrication and characterization of antibacterial herbal drug-loaded polylactic acid/cellulose acetate composite nanofiberous for wound dressing application. |
| title_fullStr | Fabrication and characterization of antibacterial herbal drug-loaded polylactic acid/cellulose acetate composite nanofiberous for wound dressing application. |
| title_full_unstemmed | Fabrication and characterization of antibacterial herbal drug-loaded polylactic acid/cellulose acetate composite nanofiberous for wound dressing application. |
| title_short | Fabrication and characterization of antibacterial herbal drug-loaded polylactic acid/cellulose acetate composite nanofiberous for wound dressing application. |
| title_sort | fabrication and characterization of antibacterial herbal drug loaded polylactic acid cellulose acetate composite nanofiberous for wound dressing application |
| topic | Wound dressing Polymeric nanofibers |
| url | https://fount.aucegypt.edu/etds/204 https://fount.aucegypt.edu/context/etds/article/1203/viewcontent/First_20draft_20animal_20study.pdf |
| work_keys_str_mv | AT khalifasalmafouad fabricationandcharacterizationofantibacterialherbaldrugloadedpolylacticacidcelluloseacetatecompositenanofiberousforwounddressingapplication |