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Fabrication of pomegranate peel extract/honey nanofibers loaded with bee venom as effective antibacterial wound dressings
In spite of the emerging advances in the field of wound dressings, there is still a deep need for the development of novel natural based dressings to offer a safe alternative for traditional wound dressings and most importantly fight against antibacterial resistance. The aim of this study is to deve...
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AUC Knowledge Fountain
2019
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| _version_ | 1867613410539077632 |
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| access_status_str | Open Access |
| author | Abouzekry, Sara |
| author_browse | Abouzekry, Sara |
| author_facet | Abouzekry, Sara |
| author_sort | Abouzekry, Sara |
| collection | Thesis |
| dc_rights_str_mv | The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. |
| description | In spite of the emerging advances in the field of wound dressings, there is still a deep need for the development of novel natural based dressings to offer a safe alternative for traditional wound dressings and most importantly fight against antibacterial resistance. The aim of this study is to develop novel honey based nanofibrous wound dressing for achieving both effective and safe treatment and investigate the dressings for antibacterial activity, cytotoxicity and in vivo wound healing effect in a rat wound model. Natural products; Honey, Pomegranate peel extract (PPP), and bee venom (BV), known for their antibacterial and anti-inflammatory properties, were used in combination with Polyvinyl alcohol (PVA), to develop a novel natural-based nanofibrous wound dressing. Methanolic pomegranate peel extract was prepared and mixed with either Manuka honey (MH) or lyophilized multiflora honey powder (LH). By testing electrospun samples: (10% MH/ 1% PPP), (20% MH/ 2% PPP) and (25%/ 2.5%PPP) against S. aureus, it was shown that antibacterial activity increases with increasing the MH/PPP concentration. BV was added to the honey/PPP combination to prepare (25 % MH/2.5 % PPP/ 0.01%BV) and (25 % LH/2.5 % PPP/0.01% BV) nanofibers. Scanning electron microscopy (SEM) showed that all samples had good morphology with no beads. Samples showed moderate swelling capacity in comparison to PVA while all samples showed better water loss capacity than PVA. Antibacterial tests showed significant antibacterial activity against both strains tested compared to both controls used (P< 0.0001). Against S. aureus, samples containing BV were slightly more effective than the sample without BV (P < 0.05). Against E. coli, sample (MH/PPP/BV) was slightly more effective than sample (LH/PPP/BV) (P < 0.05). In the cytotoxicity assay, all samples showed 100 - 120 % viability which indicated that the produced dressings have no significant cytotoxic effects. Results of the in vivo wound healing assay showed that treatment groups (25% MH/2.5%PPP), (25% MH/2.5%PPP/ 0.01 %BV), and (25% LH/2.5 %PPP/ 0.01% BV) had a significantly decreased wound surface areas compared to both controls at days 3 and day 5 (P <0.0001). All treatment groups reached complete healing by day 10 compared to day 14 in the case of both controls. On the histological side, PVA control group showed poor healing compared to all treatment groups. MH/PPP/BV sample micrographs showed excellent healing at day 10 resembling intact skin as shown by histological assessment. These results indicate that MH/PPP/BV combination can be considered as a promising formula to promote wound healing. However, further analysis is required to confirm the results and address the potential of the combination on more challenging wounds. |
| format | Thesis |
| id | oai:fount.aucegypt.edu:etds-1517 |
| institution | American University in Cairo (Egypt) |
| last_indexed | 2026-06-10T12:35:42.290Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from AUC Knowledge Fountain — bepress |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | AUC Knowledge Fountain |
| publisherStr | AUC Knowledge Fountain |
| record_format | dspace |
| source_str | AUC Knowledge Fountain — bepress |
| spelling | oai:fount.aucegypt.edu:etds-1517 Fabrication of pomegranate peel extract/honey nanofibers loaded with bee venom as effective antibacterial wound dressings Abouzekry, Sara In spite of the emerging advances in the field of wound dressings, there is still a deep need for the development of novel natural based dressings to offer a safe alternative for traditional wound dressings and most importantly fight against antibacterial resistance. The aim of this study is to develop novel honey based nanofibrous wound dressing for achieving both effective and safe treatment and investigate the dressings for antibacterial activity, cytotoxicity and in vivo wound healing effect in a rat wound model. Natural products; Honey, Pomegranate peel extract (PPP), and bee venom (BV), known for their antibacterial and anti-inflammatory properties, were used in combination with Polyvinyl alcohol (PVA), to develop a novel natural-based nanofibrous wound dressing. Methanolic pomegranate peel extract was prepared and mixed with either Manuka honey (MH) or lyophilized multiflora honey powder (LH). By testing electrospun samples: (10% MH/ 1% PPP), (20% MH/ 2% PPP) and (25%/ 2.5%PPP) against S. aureus, it was shown that antibacterial activity increases with increasing the MH/PPP concentration. BV was added to the honey/PPP combination to prepare (25 % MH/2.5 % PPP/ 0.01%BV) and (25 % LH/2.5 % PPP/0.01% BV) nanofibers. Scanning electron microscopy (SEM) showed that all samples had good morphology with no beads. Samples showed moderate swelling capacity in comparison to PVA while all samples showed better water loss capacity than PVA. Antibacterial tests showed significant antibacterial activity against both strains tested compared to both controls used (P< 0.0001). Against S. aureus, samples containing BV were slightly more effective than the sample without BV (P < 0.05). Against E. coli, sample (MH/PPP/BV) was slightly more effective than sample (LH/PPP/BV) (P < 0.05). In the cytotoxicity assay, all samples showed 100 - 120 % viability which indicated that the produced dressings have no significant cytotoxic effects. Results of the in vivo wound healing assay showed that treatment groups (25% MH/2.5%PPP), (25% MH/2.5%PPP/ 0.01 %BV), and (25% LH/2.5 %PPP/ 0.01% BV) had a significantly decreased wound surface areas compared to both controls at days 3 and day 5 (P <0.0001). All treatment groups reached complete healing by day 10 compared to day 14 in the case of both controls. On the histological side, PVA control group showed poor healing compared to all treatment groups. MH/PPP/BV sample micrographs showed excellent healing at day 10 resembling intact skin as shown by histological assessment. These results indicate that MH/PPP/BV combination can be considered as a promising formula to promote wound healing. However, further analysis is required to confirm the results and address the potential of the combination on more challenging wounds. 2019-02-01T08:00:00Z thesis application/pdf https://fount.aucegypt.edu/etds/518 https://fount.aucegypt.edu/context/etds/article/1517/viewcontent/Sara_20final_20thesis_20103.pdf The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. Theses and Dissertations AUC Knowledge Fountain Nanofibers Wound healing honey |
| spellingShingle | Nanofibers Wound healing honey Abouzekry, Sara Fabrication of pomegranate peel extract/honey nanofibers loaded with bee venom as effective antibacterial wound dressings |
| title | Fabrication of pomegranate peel extract/honey nanofibers loaded with bee venom as effective antibacterial wound dressings |
| title_full | Fabrication of pomegranate peel extract/honey nanofibers loaded with bee venom as effective antibacterial wound dressings |
| title_fullStr | Fabrication of pomegranate peel extract/honey nanofibers loaded with bee venom as effective antibacterial wound dressings |
| title_full_unstemmed | Fabrication of pomegranate peel extract/honey nanofibers loaded with bee venom as effective antibacterial wound dressings |
| title_short | Fabrication of pomegranate peel extract/honey nanofibers loaded with bee venom as effective antibacterial wound dressings |
| title_sort | fabrication of pomegranate peel extract honey nanofibers loaded with bee venom as effective antibacterial wound dressings |
| topic | Nanofibers Wound healing honey |
| url | https://fount.aucegypt.edu/etds/518 https://fount.aucegypt.edu/context/etds/article/1517/viewcontent/Sara_20final_20thesis_20103.pdf |
| work_keys_str_mv | AT abouzekrysara fabricationofpomegranatepeelextracthoneynanofibersloadedwithbeevenomaseffectiveantibacterialwounddressings |