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Therapeutic potential of curcumin in a spinal cord injury model: Local application versus dietary supplement
Spinal cord injury is a debilitating disability that is characterized by a sequence of tragic events that occur following the primary impact aggravating the condition, collectively called secondary spinal cord injury. Oxidative damage and inflammatory surge are two hallmarks of the secondary spinal...
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| Format: | Thesis |
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AUC Knowledge Fountain
2018
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| Summary: | Spinal cord injury is a debilitating disability that is characterized by a sequence of tragic events that occur following the primary impact aggravating the condition, collectively called secondary spinal cord injury. Oxidative damage and inflammatory surge are two hallmarks of the secondary spinal cord injury cascade. Curcumin is a polyphenolic compound extracted from the rhizome of Curcuma longa that has been well known to possess antioxidant and anti-inflammatory properties. The objective of this study was to evaluate the potential of curcumin as an antioxidant and anti-inflammatory agent following spinal cord injury, and to compare its therapeutic effects following local application directly to the injury versus its effect when given as a dietary supplement in a spinal cord hemisection model at T9-T10 level of the spinal cord. Female Sprague Dawley rats were randomized into a control group, injury groups (1 day and 7 days) , local treatment groups single dose of Curcuma longa extract immediately on the injury site (1 day and 7 days) and a Dietary supplement group. Crude Curcumin was added to the animals' feed (10% of daily feed) one week before and week after injury. Oxidative stress parameters used for detection the effect of Curcumin before and after treatment were Malondialdehyde (MDA) by Thiobarbituric acid assay (TBA) and total antioxidant capacity (TAC). Expression of tumor necrosis factor alpha (TNF α) and interleukin 6 (IL-6) was detected using Enzyme Linked Immunosorbent Assay (ELISA). Our results show that at 7 days, although Dietary supplement was effective in increasing TAC levels and lowering TNF α expression levels, yet it did not affect MDA levels (IL-6 data not measured). Local treatment regimen has shown to be more effective on all four parameters measured as the 7 days. Our results demonstrate that local Curcumin application directly on the injury site might be more efficacious in alleviating oxidative damage and reducing inflammation following spinal cord injury. Further analysis is needed to evaluate the effect of Dietary treatment regimen on IL-6 and detect the effect of different Curcumin treatment regimens on other anti-oxidant and anti-inflammatory markers to investigate the role of curcumin in alleviating oxidation and inflammation. |
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