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Fabrication of layer-by-layer ECM/polysaccharides/electrospun composite nanofibrous scaffolds and hydrogels for in-vitro skin cell regeneration
Skin burn wounds is a crucial issue that could reduce life quality. The novel tri-layered asymmetric porous scaffold has been innovated to mimic the natural skin layers. The scaffold constitutes of upper electrospun Cs-PVA layer and lower xerogel layer that made of effective skin ECM components. Bot...
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2019
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| _version_ | 1867613411877060608 |
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| access_status_str | Open Access |
| author | El-Shishiny, Fatma Nabil |
| author_browse | El-Shishiny, Fatma Nabil |
| author_facet | El-Shishiny, Fatma Nabil |
| author_sort | El-Shishiny, Fatma Nabil |
| collection | Thesis |
| dc_rights_str_mv | The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. |
| description | Skin burn wounds is a crucial issue that could reduce life quality. The novel tri-layered asymmetric porous scaffold has been innovated to mimic the natural skin layers. The scaffold constitutes of upper electrospun Cs-PVA layer and lower xerogel layer that made of effective skin ECM components. Both layers were stuck together by fibrin glue. The developed scaffold resulted in promising swelling capability suitable for absorbing wound exudates, followed by constant degradable weight over time which is appropriate for burn wounds environment. SEM images revealed the presence of significant pore distribution among the electrospun and xerogel layers confirmed by BET analysis. The electrospun nanofibrous layer was examined for its antibacterial property and showed expressive complete bacterial inhibition against gram-negative and gram-positive bacterial strains. Mouse embryonic fibroblast cytotoxicity and migration rate were investigated against the created asymmetrical composite. The outcome results of tissue culture experiments demonstrated significant cell proliferation and migration in presence of the constructed scaffold (P<0.0001). Complete wound closure was observed in-vitro in presence of the three scaffold asymmetrical layers against mouse embryonic fibroblast. Results of this study have been proved the superior biological characteristics of the innovated asymmetrical composite, that could further replace the burned skin layers with potential for clinical applications. |
| format | Thesis |
| id | oai:fount.aucegypt.edu:etds-1726 |
| institution | American University in Cairo (Egypt) |
| last_indexed | 2026-06-10T12:35:43.583Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from AUC Knowledge Fountain — bepress |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | AUC Knowledge Fountain |
| publisherStr | AUC Knowledge Fountain |
| record_format | dspace |
| source_str | AUC Knowledge Fountain — bepress |
| spelling | oai:fount.aucegypt.edu:etds-1726 Fabrication of layer-by-layer ECM/polysaccharides/electrospun composite nanofibrous scaffolds and hydrogels for in-vitro skin cell regeneration El-Shishiny, Fatma Nabil Skin burn wounds is a crucial issue that could reduce life quality. The novel tri-layered asymmetric porous scaffold has been innovated to mimic the natural skin layers. The scaffold constitutes of upper electrospun Cs-PVA layer and lower xerogel layer that made of effective skin ECM components. Both layers were stuck together by fibrin glue. The developed scaffold resulted in promising swelling capability suitable for absorbing wound exudates, followed by constant degradable weight over time which is appropriate for burn wounds environment. SEM images revealed the presence of significant pore distribution among the electrospun and xerogel layers confirmed by BET analysis. The electrospun nanofibrous layer was examined for its antibacterial property and showed expressive complete bacterial inhibition against gram-negative and gram-positive bacterial strains. Mouse embryonic fibroblast cytotoxicity and migration rate were investigated against the created asymmetrical composite. The outcome results of tissue culture experiments demonstrated significant cell proliferation and migration in presence of the constructed scaffold (P<0.0001). Complete wound closure was observed in-vitro in presence of the three scaffold asymmetrical layers against mouse embryonic fibroblast. Results of this study have been proved the superior biological characteristics of the innovated asymmetrical composite, that could further replace the burned skin layers with potential for clinical applications. 2019-02-01T08:00:00Z thesis text/html https://fount.aucegypt.edu/etds/727 https://fount.aucegypt.edu/context/etds/article/1726/type/native/viewcontent/FatmaEl_Shishiny_Thesis.pdf_sequence_1 The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. Theses and Dissertations AUC Knowledge Fountain Polymers Nanofibers Hydrogels Xerogels Tissue Culture Skin Regeneration Scaffolds Biomedical Engineering |
| spellingShingle | Polymers Nanofibers Hydrogels Xerogels Tissue Culture Skin Regeneration Scaffolds Biomedical Engineering El-Shishiny, Fatma Nabil Fabrication of layer-by-layer ECM/polysaccharides/electrospun composite nanofibrous scaffolds and hydrogels for in-vitro skin cell regeneration |
| title | Fabrication of layer-by-layer ECM/polysaccharides/electrospun composite nanofibrous scaffolds and hydrogels for in-vitro skin cell regeneration |
| title_full | Fabrication of layer-by-layer ECM/polysaccharides/electrospun composite nanofibrous scaffolds and hydrogels for in-vitro skin cell regeneration |
| title_fullStr | Fabrication of layer-by-layer ECM/polysaccharides/electrospun composite nanofibrous scaffolds and hydrogels for in-vitro skin cell regeneration |
| title_full_unstemmed | Fabrication of layer-by-layer ECM/polysaccharides/electrospun composite nanofibrous scaffolds and hydrogels for in-vitro skin cell regeneration |
| title_short | Fabrication of layer-by-layer ECM/polysaccharides/electrospun composite nanofibrous scaffolds and hydrogels for in-vitro skin cell regeneration |
| title_sort | fabrication of layer by layer ecm polysaccharides electrospun composite nanofibrous scaffolds and hydrogels for in vitro skin cell regeneration |
| topic | Polymers Nanofibers Hydrogels Xerogels Tissue Culture Skin Regeneration Scaffolds Biomedical Engineering |
| url | https://fount.aucegypt.edu/etds/727 https://fount.aucegypt.edu/context/etds/article/1726/type/native/viewcontent/FatmaEl_Shishiny_Thesis.pdf_sequence_1 |
| work_keys_str_mv | AT elshishinyfatmanabil fabricationoflayerbylayerecmpolysaccharideselectrospuncompositenanofibrousscaffoldsandhydrogelsforinvitroskincellregeneration |