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Characterization of variant strains of respiratory adenoviruses isolated in Egypt from 2003 to 2010
Human adenovirus (HAdV) has been associated with acute respiratory disease (ARD) in adult and pediatric patients in Egypt. Worldwide, adenovirus of species B, C, and E are predominantly associated with ARD. As part of an influenza-like illness (ILI) surveillance program in Egypt, we isolated 99 ad...
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2011
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| access_status_str | Open Access |
| author | Demian, Pola Nashdy |
| author_browse | Demian, Pola Nashdy |
| author_facet | Demian, Pola Nashdy |
| author_sort | Demian, Pola Nashdy |
| collection | Thesis |
| dc_rights_str_mv | The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. |
| description | Human adenovirus (HAdV) has been associated with acute respiratory disease (ARD) in adult and pediatric patients in Egypt. Worldwide, adenovirus of species B, C, and E are predominantly associated with ARD. As part of an influenza-like illness (ILI) surveillance program in Egypt, we isolated 99 adenoviruses from 2003 to 2010. Initial identification of the isolates was accomplished with immunofluorescence assays (IFAs) and then confirmed with multiplex and monoplex PCR protocols. Of the 99 isolates, 39 were species B, 54 were species C, and 1 was species E. Additionally, 5 isolates represented co-infections of both HAdV-B and HAdV-C. Molecular typing by PCR and sequencing of the hexon gene allowed the identification of the serotypes of the HAdV-B isolates as follows: 17 isolates were Ad-3, 14 were Ad-7, 8 were Ad-11a. For the species C HAdV, 19 isolates were Ad-1, nine were Ad-2, 13 were Ad-5 and 12 were Ad-6. One sample in this group also represented a co-infection of Ad-5/Ad-6. The serotypes of the five HAdV-B/HAdV-C co-infections were Ads (3+1) for three cases, while the other two were Ads (3+5) and Ads (3+2). In contrast to previous studies in Egypt, we observed a higher number of HAdV-Cs which was recovered largely from a pediatric population. To reveal any recombination events in the AdV-C species isolates, we sequenced the fibre and the HVR-7 of the hexon genes and detected an Ad-2/Ad-6 recombination. The isolates which showed recombination events had the fibre of Ad-6 and the HVR-7 of Ad-2. For the HAdV-C fibre genes sequenced, the tail region was highly conserved (100% homology of amino acids) whereas the shaft and knob regions showed a high degree of heterogeneity at the nucleotide and amino acids levels. Additional studies are currently underway to determine novel markers of antiviral resistance, and to define possible vaccine candidate components that would be effective for the region. |
| format | Thesis |
| id | oai:fount.aucegypt.edu:etds-2169 |
| institution | American University in Cairo (Egypt) |
| last_indexed | 2026-06-10T12:35:47.730Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from AUC Knowledge Fountain — bepress |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| publisher | AUC Knowledge Fountain |
| publisherStr | AUC Knowledge Fountain |
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| spelling | oai:fount.aucegypt.edu:etds-2169 Characterization of variant strains of respiratory adenoviruses isolated in Egypt from 2003 to 2010 Demian, Pola Nashdy Human adenovirus (HAdV) has been associated with acute respiratory disease (ARD) in adult and pediatric patients in Egypt. Worldwide, adenovirus of species B, C, and E are predominantly associated with ARD. As part of an influenza-like illness (ILI) surveillance program in Egypt, we isolated 99 adenoviruses from 2003 to 2010. Initial identification of the isolates was accomplished with immunofluorescence assays (IFAs) and then confirmed with multiplex and monoplex PCR protocols. Of the 99 isolates, 39 were species B, 54 were species C, and 1 was species E. Additionally, 5 isolates represented co-infections of both HAdV-B and HAdV-C. Molecular typing by PCR and sequencing of the hexon gene allowed the identification of the serotypes of the HAdV-B isolates as follows: 17 isolates were Ad-3, 14 were Ad-7, 8 were Ad-11a. For the species C HAdV, 19 isolates were Ad-1, nine were Ad-2, 13 were Ad-5 and 12 were Ad-6. One sample in this group also represented a co-infection of Ad-5/Ad-6. The serotypes of the five HAdV-B/HAdV-C co-infections were Ads (3+1) for three cases, while the other two were Ads (3+5) and Ads (3+2). In contrast to previous studies in Egypt, we observed a higher number of HAdV-Cs which was recovered largely from a pediatric population. To reveal any recombination events in the AdV-C species isolates, we sequenced the fibre and the HVR-7 of the hexon genes and detected an Ad-2/Ad-6 recombination. The isolates which showed recombination events had the fibre of Ad-6 and the HVR-7 of Ad-2. For the HAdV-C fibre genes sequenced, the tail region was highly conserved (100% homology of amino acids) whereas the shaft and knob regions showed a high degree of heterogeneity at the nucleotide and amino acids levels. Additional studies are currently underway to determine novel markers of antiviral resistance, and to define possible vaccine candidate components that would be effective for the region. 2011-06-01T07:00:00Z thesis application/pdf https://fount.aucegypt.edu/etds/1170 https://fount.aucegypt.edu/context/etds/article/2169/viewcontent/ETD_2011_Spring_Pola_Demian_Thesis.pdf The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. Theses and Dissertations AUC Knowledge Fountain Adenovirus Respiratory |
| spellingShingle | Adenovirus Respiratory Demian, Pola Nashdy Characterization of variant strains of respiratory adenoviruses isolated in Egypt from 2003 to 2010 |
| title | Characterization of variant strains of respiratory adenoviruses isolated in Egypt from 2003 to 2010 |
| title_full | Characterization of variant strains of respiratory adenoviruses isolated in Egypt from 2003 to 2010 |
| title_fullStr | Characterization of variant strains of respiratory adenoviruses isolated in Egypt from 2003 to 2010 |
| title_full_unstemmed | Characterization of variant strains of respiratory adenoviruses isolated in Egypt from 2003 to 2010 |
| title_short | Characterization of variant strains of respiratory adenoviruses isolated in Egypt from 2003 to 2010 |
| title_sort | characterization of variant strains of respiratory adenoviruses isolated in egypt from 2003 to 2010 |
| topic | Adenovirus Respiratory |
| url | https://fount.aucegypt.edu/etds/1170 https://fount.aucegypt.edu/context/etds/article/2169/viewcontent/ETD_2011_Spring_Pola_Demian_Thesis.pdf |
| work_keys_str_mv | AT demianpolanashdy characterizationofvariantstrainsofrespiratoryadenovirusesisolatedinegyptfrom2003to2010 |