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Towards the characterization of a novel thermohalophilic antioxidant Thioredoxin from the metagenome of the Red Sea; LCL of Atlantis II Brine pool
Due to the crucial antioxidant role of Thioredoxin (Trx) system in various vital cellular processes; DNA synthesis, oxidative stress defense, protein folding, apoptosis and cell growth, this fundamental system is widely expressed in mostly all life's kingdoms. Thus using metagenomic approaches to ch...
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2014
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| _version_ | 1867613416444657664 |
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| access_status_str | Open Access |
| author | Abdelwahed, Mohamed Mohamed Gamal |
| author_browse | Abdelwahed, Mohamed Mohamed Gamal |
| author_facet | Abdelwahed, Mohamed Mohamed Gamal |
| author_sort | Abdelwahed, Mohamed Mohamed Gamal |
| collection | Thesis |
| dc_rights_str_mv | The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. |
| description | Due to the crucial antioxidant role of Thioredoxin (Trx) system in various vital cellular processes; DNA synthesis, oxidative stress defense, protein folding, apoptosis and cell growth, this fundamental system is widely expressed in mostly all life's kingdoms. Thus using metagenomic approaches to characterize novel Trx system in unexplored harsh environment will open the window for understanding the evolution of Trx system and its unique adaptation in extreme habitations. One of the unexplored unique ecosystems is the Red Sea's Atalntis II brine pool, specifically, the lower convective layer (LCL). The exceptional harsh conditions of the LCL; anoxic condition, high temperature around 70°C, high salinity (26%), and high metal content, have a significant contribution for being a unique infrequent ecosystem. The objective of this study is to characterize a novel Trx isolated from the LCL. Successfully, in the experimental part, Trx was expressed in E. coli and purified, where the purified Trx has shown a clear antioxidant activity with a unique thermohalophilicity. In conclusion, we have characterized a unique antioxidant Trx protein. |
| format | Thesis |
| id | oai:fount.aucegypt.edu:etds-2190 |
| institution | American University in Cairo (Egypt) |
| last_indexed | 2026-06-10T12:35:47.730Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from AUC Knowledge Fountain — bepress |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | AUC Knowledge Fountain |
| publisherStr | AUC Knowledge Fountain |
| record_format | dspace |
| source_str | AUC Knowledge Fountain — bepress |
| spelling | oai:fount.aucegypt.edu:etds-2190 Towards the characterization of a novel thermohalophilic antioxidant Thioredoxin from the metagenome of the Red Sea; LCL of Atlantis II Brine pool Abdelwahed, Mohamed Mohamed Gamal Due to the crucial antioxidant role of Thioredoxin (Trx) system in various vital cellular processes; DNA synthesis, oxidative stress defense, protein folding, apoptosis and cell growth, this fundamental system is widely expressed in mostly all life's kingdoms. Thus using metagenomic approaches to characterize novel Trx system in unexplored harsh environment will open the window for understanding the evolution of Trx system and its unique adaptation in extreme habitations. One of the unexplored unique ecosystems is the Red Sea's Atalntis II brine pool, specifically, the lower convective layer (LCL). The exceptional harsh conditions of the LCL; anoxic condition, high temperature around 70°C, high salinity (26%), and high metal content, have a significant contribution for being a unique infrequent ecosystem. The objective of this study is to characterize a novel Trx isolated from the LCL. Successfully, in the experimental part, Trx was expressed in E. coli and purified, where the purified Trx has shown a clear antioxidant activity with a unique thermohalophilicity. In conclusion, we have characterized a unique antioxidant Trx protein. 2014-06-01T07:00:00Z thesis application/pdf https://fount.aucegypt.edu/etds/1191 https://fount.aucegypt.edu/context/etds/article/2190/viewcontent/Final_20Thesis_20Correction_2021_20May_202014.pdf The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. Theses and Dissertations AUC Knowledge Fountain Atlantis II (Ship : 1963-1996) Red Sea |
| spellingShingle | Atlantis II (Ship : 1963-1996) Red Sea Abdelwahed, Mohamed Mohamed Gamal Towards the characterization of a novel thermohalophilic antioxidant Thioredoxin from the metagenome of the Red Sea; LCL of Atlantis II Brine pool |
| title | Towards the characterization of a novel thermohalophilic antioxidant Thioredoxin from the metagenome of the Red Sea; LCL of Atlantis II Brine pool |
| title_full | Towards the characterization of a novel thermohalophilic antioxidant Thioredoxin from the metagenome of the Red Sea; LCL of Atlantis II Brine pool |
| title_fullStr | Towards the characterization of a novel thermohalophilic antioxidant Thioredoxin from the metagenome of the Red Sea; LCL of Atlantis II Brine pool |
| title_full_unstemmed | Towards the characterization of a novel thermohalophilic antioxidant Thioredoxin from the metagenome of the Red Sea; LCL of Atlantis II Brine pool |
| title_short | Towards the characterization of a novel thermohalophilic antioxidant Thioredoxin from the metagenome of the Red Sea; LCL of Atlantis II Brine pool |
| title_sort | towards the characterization of a novel thermohalophilic antioxidant thioredoxin from the metagenome of the red sea lcl of atlantis ii brine pool |
| topic | Atlantis II (Ship : 1963-1996) Red Sea |
| url | https://fount.aucegypt.edu/etds/1191 https://fount.aucegypt.edu/context/etds/article/2190/viewcontent/Final_20Thesis_20Correction_2021_20May_202014.pdf |
| work_keys_str_mv | AT abdelwahedmohamedmohamedgamal towardsthecharacterizationofanovelthermohalophilicantioxidantthioredoxinfromthemetagenomeoftheredsealclofatlantisiibrinepool |