Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Anti-schistosoma single-domain antibody-nanoparticles conjugate: a novel tool for diagnostic and therapeutic applications
Nanotechnology has enthused excessive expectations in recent years, particularly in the biology and biomedical fields. Carbon-coated metallic nanomagnets reveal significant physicochemical properties, which are referred to as superparamagnetism, that when designed appropriately can be utilized to ge...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Published: |
AUC Knowledge Fountain
2013
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613417312878592 |
|---|---|
| access_status_str | Open Access |
| author | Sallam, Mohamed Abdelazim |
| author_browse | Sallam, Mohamed Abdelazim |
| author_facet | Sallam, Mohamed Abdelazim |
| author_sort | Sallam, Mohamed Abdelazim |
| collection | Thesis |
| dc_rights_str_mv | The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. |
| description | Nanotechnology has enthused excessive expectations in recent years, particularly in the biology and biomedical fields. Carbon-coated metallic nanomagnets reveal significant physicochemical properties, which are referred to as superparamagnetism, that when designed appropriately can be utilized to generate novel diagnostic and therapeutic applications for a wide range of biologically hazardous species. Schistosomiasis is a chronic parasitic disease that can infect both humans and animals, particularly cattle. It is caused by a trematode blood fluke of the genus Schistosoma which belong to the schistosomatidae family. At least 200 million individuals are infected in 75 countries while 600 million people are exposed to infection and are potentially victim of this parasite. Schistosomiasis is still recognized as one of the most neglected disease, the current available diagnostic techniques are deficient in accuracy and are incapable of identifying the disease in its early stages. Moreover, they suffer from prolonged examination time. Besides, the poorly available therapeutic drugs started to lose their efficacy and the parasite started to develop resistance against most of them, which create an imperative need for developing novel diagnostic and therapeutic tools. The humoral immune response of the Camelidae is unique since these animals possess functional heavy-chain only antibodies in addition to the classical antibodies. Thanks to recombinant DNA technology, a series of single domain antigen binding entities can be produced against specific schistosomeâ s parasite antigens. These binders possess a number of distinctive biophysical properties that offer particular advantages in various theranostic applications. Finally, nanomagnetism was selected to represent a certain phenomena through which we demonstrated, to our knowledge for the first time, the successful construction of effective and functional bioconjugate system which is appropriate for a plethora of diagnostic and therapeutic applications. The main thrust of this research work was towards the developing of single domain antibodies against Schistosoma mansoni gut specific antigen and execratory secretory antigen. In parallel, state-of-the-art methodologies were implemented for constructing a bioconjugate system composed of sdAb and graphene-coated metallic nanomagnet tagged with a carboxyl terminal. Finally, the well-established metastable technetium-99 (99mTc) is used for labeling the conjugate for studying the possibility of active and magnetic targeting of the nano-structured system i.e. 99mTc-sdAb-Fe@C-MNP conjugates. |
| format | Thesis |
| id | oai:fount.aucegypt.edu:etds-2278 |
| institution | American University in Cairo (Egypt) |
| last_indexed | 2026-06-10T12:35:48.888Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from AUC Knowledge Fountain — bepress |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | AUC Knowledge Fountain |
| publisherStr | AUC Knowledge Fountain |
| record_format | dspace |
| source_str | AUC Knowledge Fountain — bepress |
| spelling | oai:fount.aucegypt.edu:etds-2278 Anti-schistosoma single-domain antibody-nanoparticles conjugate: a novel tool for diagnostic and therapeutic applications Sallam, Mohamed Abdelazim Nanotechnology has enthused excessive expectations in recent years, particularly in the biology and biomedical fields. Carbon-coated metallic nanomagnets reveal significant physicochemical properties, which are referred to as superparamagnetism, that when designed appropriately can be utilized to generate novel diagnostic and therapeutic applications for a wide range of biologically hazardous species. Schistosomiasis is a chronic parasitic disease that can infect both humans and animals, particularly cattle. It is caused by a trematode blood fluke of the genus Schistosoma which belong to the schistosomatidae family. At least 200 million individuals are infected in 75 countries while 600 million people are exposed to infection and are potentially victim of this parasite. Schistosomiasis is still recognized as one of the most neglected disease, the current available diagnostic techniques are deficient in accuracy and are incapable of identifying the disease in its early stages. Moreover, they suffer from prolonged examination time. Besides, the poorly available therapeutic drugs started to lose their efficacy and the parasite started to develop resistance against most of them, which create an imperative need for developing novel diagnostic and therapeutic tools. The humoral immune response of the Camelidae is unique since these animals possess functional heavy-chain only antibodies in addition to the classical antibodies. Thanks to recombinant DNA technology, a series of single domain antigen binding entities can be produced against specific schistosomeâ s parasite antigens. These binders possess a number of distinctive biophysical properties that offer particular advantages in various theranostic applications. Finally, nanomagnetism was selected to represent a certain phenomena through which we demonstrated, to our knowledge for the first time, the successful construction of effective and functional bioconjugate system which is appropriate for a plethora of diagnostic and therapeutic applications. The main thrust of this research work was towards the developing of single domain antibodies against Schistosoma mansoni gut specific antigen and execratory secretory antigen. In parallel, state-of-the-art methodologies were implemented for constructing a bioconjugate system composed of sdAb and graphene-coated metallic nanomagnet tagged with a carboxyl terminal. Finally, the well-established metastable technetium-99 (99mTc) is used for labeling the conjugate for studying the possibility of active and magnetic targeting of the nano-structured system i.e. 99mTc-sdAb-Fe@C-MNP conjugates. 2013-02-01T08:00:00Z thesis application/pdf https://fount.aucegypt.edu/etds/1279 https://fount.aucegypt.edu/context/etds/article/2278/viewcontent/FINAL_20THESIS.pdf The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. Theses and Dissertations AUC Knowledge Fountain noparticles Bionotechnology Single domain antibodies Heavy chain only antibodies nobodies 99mTc radiolabeling Schistosoma mansoni Diagnosis and Therapeutic |
| spellingShingle | noparticles Bionotechnology Single domain antibodies Heavy chain only antibodies nobodies 99mTc radiolabeling Schistosoma mansoni Diagnosis and Therapeutic Sallam, Mohamed Abdelazim Anti-schistosoma single-domain antibody-nanoparticles conjugate: a novel tool for diagnostic and therapeutic applications |
| title | Anti-schistosoma single-domain antibody-nanoparticles conjugate: a novel tool for diagnostic and therapeutic applications |
| title_full | Anti-schistosoma single-domain antibody-nanoparticles conjugate: a novel tool for diagnostic and therapeutic applications |
| title_fullStr | Anti-schistosoma single-domain antibody-nanoparticles conjugate: a novel tool for diagnostic and therapeutic applications |
| title_full_unstemmed | Anti-schistosoma single-domain antibody-nanoparticles conjugate: a novel tool for diagnostic and therapeutic applications |
| title_short | Anti-schistosoma single-domain antibody-nanoparticles conjugate: a novel tool for diagnostic and therapeutic applications |
| title_sort | anti schistosoma single domain antibody nanoparticles conjugate a novel tool for diagnostic and therapeutic applications |
| topic | noparticles Bionotechnology Single domain antibodies Heavy chain only antibodies nobodies 99mTc radiolabeling Schistosoma mansoni Diagnosis and Therapeutic |
| url | https://fount.aucegypt.edu/etds/1279 https://fount.aucegypt.edu/context/etds/article/2278/viewcontent/FINAL_20THESIS.pdf |
| work_keys_str_mv | AT sallammohamedabdelazim antischistosomasingledomainantibodynanoparticlesconjugateanoveltoolfordiagnosticandtherapeuticapplications |