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Novel magnetic delivery systems for platinum anticancer drugs
Carboplatin's success in the treatment of major types of cancer has been attributed to its ease of administration and its high therapeutic index. However, despite its superior toxicity profile, myelosuppression remains to be its dose-limiting side effect. Liposomes have been researched extensively a...
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2019
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| _version_ | 1867613420520472576 |
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| access_status_str | Open Access |
| author | Refaat, Lamis Alaa Eldin |
| author_browse | Refaat, Lamis Alaa Eldin |
| author_facet | Refaat, Lamis Alaa Eldin |
| author_sort | Refaat, Lamis Alaa Eldin |
| collection | Thesis |
| dc_rights_str_mv | The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. The author has granted the American University in Cairo or its agents a non-exclusive license to archive this thesis, dissertation, paper, or record of study, and to make it accessible, in whole or in part, in all forms of media, now or hereafter known. http://creativecommons.org/licenses/by-nd/4.0/ |
| description | Carboplatin's success in the treatment of major types of cancer has been attributed to its ease of administration and its high therapeutic index. However, despite its superior toxicity profile, myelosuppression remains to be its dose-limiting side effect. Liposomes have been researched extensively as carrier systems for therapeutic agents; their high biocompatibility, their ability to escape the immune system and most importantly their ability to incorporate both hydrophobic and hydrophilic drugs led to numerous liposomal formulations being designed for cancer therapy. Magnetic nanoparticles have been gaining attention as their properties enable them to be used in various therapeutic and diagnostic applications, in addition to gene and drug delivery. We report the successful preparation of magnetic liposomal formulation with particle size less than 200 nm, coated with Polyethylene Glycol, encapsulating superparamagnetic magnetite nanoparticles and carboplatin in its aqueous lumen. Two types of liposomes were prepared through thin film hydration technique; magnetite liposome and carboplatin-magnetite liposome with mean particle sizes of 183.3 ± 2.65 nm and 192.5 ± 3.42 nm respectively. Both formulations showed uniform particle size distribution and sterical stability as evident from their polydispersity index and Zeta-potential values. Encapsulation efficiency of magnetite in magnetite liposomal formulation was 61.37%, this value decreased significantly upon incorporation of carboplatin with magnetite in the second formulation. Carboplatin's EE% was found to be 13.87%, its release profile from the liposome showed a controlled release over the course of 72 hours. The prepared formulations have been successfully magnetically controlled with optimized magnetic gradient and input current strength. Additionally, in vitro tests performed on melanoma, mammary and oral squamous cell carcinoma cell lines showed significant superior cytotoxicity profile of carboplatin-magnetite liposome over free carboplatin solution. |
| format | Thesis |
| id | oai:fount.aucegypt.edu:etds-2773 |
| institution | American University in Cairo (Egypt) |
| last_indexed | 2026-06-10T12:35:51.500Z |
| license_str | Creative Commons |
| provenance_str_mv | Harvested via OAI-PMH from AUC Knowledge Fountain — bepress |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | AUC Knowledge Fountain |
| publisherStr | AUC Knowledge Fountain |
| record_format | dspace |
| source_str | AUC Knowledge Fountain — bepress |
| spelling | oai:fount.aucegypt.edu:etds-2773 Novel magnetic delivery systems for platinum anticancer drugs Refaat, Lamis Alaa Eldin Carboplatin's success in the treatment of major types of cancer has been attributed to its ease of administration and its high therapeutic index. However, despite its superior toxicity profile, myelosuppression remains to be its dose-limiting side effect. Liposomes have been researched extensively as carrier systems for therapeutic agents; their high biocompatibility, their ability to escape the immune system and most importantly their ability to incorporate both hydrophobic and hydrophilic drugs led to numerous liposomal formulations being designed for cancer therapy. Magnetic nanoparticles have been gaining attention as their properties enable them to be used in various therapeutic and diagnostic applications, in addition to gene and drug delivery. We report the successful preparation of magnetic liposomal formulation with particle size less than 200 nm, coated with Polyethylene Glycol, encapsulating superparamagnetic magnetite nanoparticles and carboplatin in its aqueous lumen. Two types of liposomes were prepared through thin film hydration technique; magnetite liposome and carboplatin-magnetite liposome with mean particle sizes of 183.3 ± 2.65 nm and 192.5 ± 3.42 nm respectively. Both formulations showed uniform particle size distribution and sterical stability as evident from their polydispersity index and Zeta-potential values. Encapsulation efficiency of magnetite in magnetite liposomal formulation was 61.37%, this value decreased significantly upon incorporation of carboplatin with magnetite in the second formulation. Carboplatin's EE% was found to be 13.87%, its release profile from the liposome showed a controlled release over the course of 72 hours. The prepared formulations have been successfully magnetically controlled with optimized magnetic gradient and input current strength. Additionally, in vitro tests performed on melanoma, mammary and oral squamous cell carcinoma cell lines showed significant superior cytotoxicity profile of carboplatin-magnetite liposome over free carboplatin solution. 2019-12-17T08:00:00Z thesis application/pdf https://fount.aucegypt.edu/etds/1741 https://fount.aucegypt.edu/context/etds/article/2773/viewcontent/Refaat.pdf The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. The author has granted the American University in Cairo or its agents a non-exclusive license to archive this thesis, dissertation, paper, or record of study, and to make it accessible, in whole or in part, in all forms of media, now or hereafter known. http://creativecommons.org/licenses/by-nd/4.0/ Theses and Dissertations AUC Knowledge Fountain Carboplatin Platinum agents |
| spellingShingle | Carboplatin Platinum agents Refaat, Lamis Alaa Eldin Novel magnetic delivery systems for platinum anticancer drugs |
| title | Novel magnetic delivery systems for platinum anticancer drugs |
| title_full | Novel magnetic delivery systems for platinum anticancer drugs |
| title_fullStr | Novel magnetic delivery systems for platinum anticancer drugs |
| title_full_unstemmed | Novel magnetic delivery systems for platinum anticancer drugs |
| title_short | Novel magnetic delivery systems for platinum anticancer drugs |
| title_sort | novel magnetic delivery systems for platinum anticancer drugs |
| topic | Carboplatin Platinum agents |
| url | https://fount.aucegypt.edu/etds/1741 https://fount.aucegypt.edu/context/etds/article/2773/viewcontent/Refaat.pdf |
| work_keys_str_mv | AT refaatlamisalaaeldin novelmagneticdeliverysystemsforplatinumanticancerdrugs |