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Formulation of Encapsulated Nanoparticles of Leek Extract as Antimicrobial and Anticancer Agent
Leek extract (LEK) has broad therapeutic activity. However, plant extract use has many drawbacks, which could be resolved using nanocarriers. The purpose of this research work was to prepare chitosan nanoparticles (CS NPs) encapsulating LEK and investigate their antioxidant, antimicrobial, and antic...
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2023
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| _version_ | 1867613422989869056 |
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| access_status_str | Open Access |
| author | Elghanam, Rawan Hassan Mohamed |
| author_browse | Elghanam, Rawan Hassan Mohamed |
| author_facet | Elghanam, Rawan Hassan Mohamed |
| author_sort | Elghanam, Rawan Hassan Mohamed |
| collection | Thesis |
| description | Leek extract (LEK) has broad therapeutic activity. However, plant extract use has many drawbacks, which could be resolved using nanocarriers. The purpose of this research work was to prepare chitosan nanoparticles (CS NPs) encapsulating LEK and investigate their antioxidant, antimicrobial, and anticancer activity. Gas chromatography-mass spectroscopy was used to determine the LEK components. The CS-LEK NPs were produced by tripolyphosphate (TPP) crosslinking with chitosan (CS) the ionic gelation methodology. The particle size was 331 nm, zeta potential (ZP) was 28.9 mv, polydispersity index (PDI) was 0.4, encapsulation efficiency (EE) was 65.6%, and loading capacity (LC) was 28.8 % of the optimum CS-LEK NPs with chitosan: LEK (1:1w/w). The TEM showed that the CS-LEK NPs had optimum dispersion with a spherical shape. The FTIR spectrum showed that the LEK was encapsulated in CS NPs. The release profile of LEK from CS-LEK NPs was performed for 72 hr.. The Folin–Ciocalteu method of the CS-LE NPs demonstrated higher total phenolic content (TPC) than the LEK and CS NPs. Also, the results of antioxidant activity of encapsulated NPs had the highest activity. Antibacterial tests for Gram-positive and Gram-negative bacteria were tested for CS NPs, CS-LEK NPs, and LEK, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were identified. For the anticancer activity of CS NPs, CS-LEK NPs, and LEK, the MTT assay was performed for three cancerous cell lines, breast cancer (MCF-7), liver cancer (HepG-2), and colorectal cancer (CACO2). Thus, the CS-LEK NPs can protect and control the delivery of LEK to overcome cancer and microbial infection. The aforementioned results demonstrated the benefits of employing CS NPs as a nanocarrier for LEK, offering a viable method for enhancing botanical-based medicines. |
| format | Thesis |
| id | oai:fount.aucegypt.edu:etds-3212 |
| institution | American University in Cairo (Egypt) |
| last_indexed | 2026-06-10T12:35:54.296Z |
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| provenance_str_mv | Harvested via OAI-PMH from AUC Knowledge Fountain — bepress |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | AUC Knowledge Fountain |
| publisherStr | AUC Knowledge Fountain |
| record_format | dspace |
| source_str | AUC Knowledge Fountain — bepress |
| spelling | oai:fount.aucegypt.edu:etds-3212 Formulation of Encapsulated Nanoparticles of Leek Extract as Antimicrobial and Anticancer Agent Elghanam, Rawan Hassan Mohamed Leek extract (LEK) has broad therapeutic activity. However, plant extract use has many drawbacks, which could be resolved using nanocarriers. The purpose of this research work was to prepare chitosan nanoparticles (CS NPs) encapsulating LEK and investigate their antioxidant, antimicrobial, and anticancer activity. Gas chromatography-mass spectroscopy was used to determine the LEK components. The CS-LEK NPs were produced by tripolyphosphate (TPP) crosslinking with chitosan (CS) the ionic gelation methodology. The particle size was 331 nm, zeta potential (ZP) was 28.9 mv, polydispersity index (PDI) was 0.4, encapsulation efficiency (EE) was 65.6%, and loading capacity (LC) was 28.8 % of the optimum CS-LEK NPs with chitosan: LEK (1:1w/w). The TEM showed that the CS-LEK NPs had optimum dispersion with a spherical shape. The FTIR spectrum showed that the LEK was encapsulated in CS NPs. The release profile of LEK from CS-LEK NPs was performed for 72 hr.. The Folin–Ciocalteu method of the CS-LE NPs demonstrated higher total phenolic content (TPC) than the LEK and CS NPs. Also, the results of antioxidant activity of encapsulated NPs had the highest activity. Antibacterial tests for Gram-positive and Gram-negative bacteria were tested for CS NPs, CS-LEK NPs, and LEK, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were identified. For the anticancer activity of CS NPs, CS-LEK NPs, and LEK, the MTT assay was performed for three cancerous cell lines, breast cancer (MCF-7), liver cancer (HepG-2), and colorectal cancer (CACO2). Thus, the CS-LEK NPs can protect and control the delivery of LEK to overcome cancer and microbial infection. The aforementioned results demonstrated the benefits of employing CS NPs as a nanocarrier for LEK, offering a viable method for enhancing botanical-based medicines. 2023-02-08T08:00:00Z thesis application/pdf https://fount.aucegypt.edu/etds/2176 https://fount.aucegypt.edu/context/etds/article/3212/viewcontent/Rawan_Hassan_Elghanam_Thesis.pdf Theses and Dissertations AUC Knowledge Fountain Antimicrobial; Anticancer; Chitosan; Leek extract; Box behnken design; Nanoparticles; Natural extract. Alternative and Complementary Medicine Biomedical Engineering and Bioengineering Chemicals and Drugs Nanomedicine Nanoscience and Nanotechnology Nanotechnology Pharmacy and Pharmaceutical Sciences |
| spellingShingle | Antimicrobial; Anticancer; Chitosan; Leek extract; Box behnken design; Nanoparticles; Natural extract. Alternative and Complementary Medicine Biomedical Engineering and Bioengineering Chemicals and Drugs Nanomedicine Nanoscience and Nanotechnology Nanotechnology Pharmacy and Pharmaceutical Sciences Elghanam, Rawan Hassan Mohamed Formulation of Encapsulated Nanoparticles of Leek Extract as Antimicrobial and Anticancer Agent |
| title | Formulation of Encapsulated Nanoparticles of Leek Extract as Antimicrobial and Anticancer Agent |
| title_full | Formulation of Encapsulated Nanoparticles of Leek Extract as Antimicrobial and Anticancer Agent |
| title_fullStr | Formulation of Encapsulated Nanoparticles of Leek Extract as Antimicrobial and Anticancer Agent |
| title_full_unstemmed | Formulation of Encapsulated Nanoparticles of Leek Extract as Antimicrobial and Anticancer Agent |
| title_short | Formulation of Encapsulated Nanoparticles of Leek Extract as Antimicrobial and Anticancer Agent |
| title_sort | formulation of encapsulated nanoparticles of leek extract as antimicrobial and anticancer agent |
| topic | Antimicrobial; Anticancer; Chitosan; Leek extract; Box behnken design; Nanoparticles; Natural extract. Alternative and Complementary Medicine Biomedical Engineering and Bioengineering Chemicals and Drugs Nanomedicine Nanoscience and Nanotechnology Nanotechnology Pharmacy and Pharmaceutical Sciences |
| url | https://fount.aucegypt.edu/etds/2176 https://fount.aucegypt.edu/context/etds/article/3212/viewcontent/Rawan_Hassan_Elghanam_Thesis.pdf |
| work_keys_str_mv | AT elghanamrawanhassanmohamed formulationofencapsulatednanoparticlesofleekextractasantimicrobialandanticanceragent |