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The DUBR/miR-210-5p/RETSAT Axis in Cholesterol-Associated Steatohepatitis: Clinical Signatures and Molecular Mechanisms
Metabolic-associated fatty liver disease (MAFLD) represents an increasing global health challenge, with cholesterol-associated steatohepatitis (CASH) emerging as a distinct pathological entity warranting investigation. While CASH demonstrates unique features compared to traditional triglyceride-driv...
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| Format: | Thesis |
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AUC Knowledge Fountain
2025
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| Summary: | Metabolic-associated fatty liver disease (MAFLD) represents an increasing global health challenge, with cholesterol-associated steatohepatitis (CASH) emerging as a distinct pathological entity warranting investigation. While CASH demonstrates unique features compared to traditional triglyceride-driven steatohepatitis, its underlying molecular mechanisms remain poorly understood. This study investigates a novel regulatory axis involving DPPA2 Upstream Binding RNA (DUBR), miR-210-5p, and retinol saturase (RETSAT). 170 patient samples revealed progressive axis dysregulation in disease states, with RETSAT emerging as a highly accurate diagnostic marker (AUC=1.000). Using novel in vitro models of MASH and CASH, we demonstrated that miR-210-5p modulation significantly affects lipid accumulation and cellular phenotype, with more pronounced effects in cholesterol-rich environments. Mechanistic studies revealed that miR-210-5p inhibition dramatically increased lipid accumulation and altered the expression of key metabolic regulators, suggesting its role as a metabolic mediator rather than the master regulator. These findings identify new molecular mechanisms in CASH pathogenesis, establish RETSAT as a potential diagnostic biomarker, and reveal promising therapeutic targets for intervention. |
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