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Nanoencapsulation of Terfezia Claveryi and Terfezia Boudieri Using Cyclodextrins for Targeted Breast Cancer Therapy
Desert truffles, particularly Terfezia claveryi and Terfezia boudieri, have long attracted scientific interest due to their rich array of bioactive compounds and potential anticancer properties. Yet, despite their promise, their use in treatment protocols is still limited by poor solubility and low...
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2026
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| _version_ | 1867613432260329472 |
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| access_status_str | Open Access |
| author | Nouh, Roua Aref |
| author_browse | Nouh, Roua Aref |
| author_facet | Nouh, Roua Aref |
| author_sort | Nouh, Roua Aref |
| collection | Thesis |
| description | Desert truffles, particularly Terfezia claveryi and Terfezia boudieri, have long attracted scientific interest due to their rich array of bioactive compounds and potential anticancer properties. Yet, despite their promise, their use in treatment protocols is still limited by poor solubility and low bioavailability.
In this research, we set out to systematically evaluate the anticancer effects of T. claveryi and T. boudieri in breast cancer models, aiming to enhance their therapeutic potential through nanoencapsulation with cyclodextrins and to explore possible synergy with doxorubicin in overcoming drug resistance.
To address these goals, the truffles extracts have been encapsulated in hydroxypropyl-β-cyclodextrin (HPβCD), seeking to improve both stability and bioavailability. The cytotoxicity of these formulations was examined via MTT assays on both drug-sensitive (MCF-7) and doxorubicin-resistant (ADR-MCF-7) breast cancer cell lines, across 24, 48, and 72-hour intervals. These assays have been complemented with flow cytometry to analyze apoptosis induction and cell cycle arrest. To take these findings a step further, we had conducted in vivo study in a mouse model, evaluating both systemic toxicity and therapeutic efficacy.
The results were clarified that nanoencapsulation with cyclodextrin led to a more sustained release and markedly improved cytotoxic effects, especially after 72 hours in vitro model. Flow cytometry confirmed significant induction of apoptosis and cell cycle disruption, with nanoformulated extracts consistently outperforming their free counterparts. |
| format | Thesis |
| id | oai:fount.aucegypt.edu:etds-3721 |
| institution | American University in Cairo (Egypt) |
| last_indexed | 2026-06-10T12:35:59.828Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from AUC Knowledge Fountain — bepress |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| publisher | AUC Knowledge Fountain |
| publisherStr | AUC Knowledge Fountain |
| record_format | dspace |
| source_str | AUC Knowledge Fountain — bepress |
| spelling | oai:fount.aucegypt.edu:etds-3721 Nanoencapsulation of Terfezia Claveryi and Terfezia Boudieri Using Cyclodextrins for Targeted Breast Cancer Therapy Nouh, Roua Aref Desert truffles, particularly Terfezia claveryi and Terfezia boudieri, have long attracted scientific interest due to their rich array of bioactive compounds and potential anticancer properties. Yet, despite their promise, their use in treatment protocols is still limited by poor solubility and low bioavailability. In this research, we set out to systematically evaluate the anticancer effects of T. claveryi and T. boudieri in breast cancer models, aiming to enhance their therapeutic potential through nanoencapsulation with cyclodextrins and to explore possible synergy with doxorubicin in overcoming drug resistance. To address these goals, the truffles extracts have been encapsulated in hydroxypropyl-β-cyclodextrin (HPβCD), seeking to improve both stability and bioavailability. The cytotoxicity of these formulations was examined via MTT assays on both drug-sensitive (MCF-7) and doxorubicin-resistant (ADR-MCF-7) breast cancer cell lines, across 24, 48, and 72-hour intervals. These assays have been complemented with flow cytometry to analyze apoptosis induction and cell cycle arrest. To take these findings a step further, we had conducted in vivo study in a mouse model, evaluating both systemic toxicity and therapeutic efficacy. The results were clarified that nanoencapsulation with cyclodextrin led to a more sustained release and markedly improved cytotoxic effects, especially after 72 hours in vitro model. Flow cytometry confirmed significant induction of apoptosis and cell cycle disruption, with nanoformulated extracts consistently outperforming their free counterparts. 2026-02-15T08:00:00Z thesis application/pdf https://fount.aucegypt.edu/etds/2723 https://fount.aucegypt.edu/context/etds/article/3721/viewcontent/Roua_Aref_Nouh_Thesis.pdf Theses and Dissertations AUC Knowledge Fountain Desert truffles Terfezia claveryi Terfezia boudieri Nanoencapsulation Cyclodextrin- nano-inclusion complexes Breast cancer cytotoxicity Chemicals and Drugs Diseases Medicine and Health Sciences Pharmacy and Pharmaceutical Sciences |
| spellingShingle | Desert truffles Terfezia claveryi Terfezia boudieri Nanoencapsulation Cyclodextrin- nano-inclusion complexes Breast cancer cytotoxicity Chemicals and Drugs Diseases Medicine and Health Sciences Pharmacy and Pharmaceutical Sciences Nouh, Roua Aref Nanoencapsulation of Terfezia Claveryi and Terfezia Boudieri Using Cyclodextrins for Targeted Breast Cancer Therapy |
| title | Nanoencapsulation of Terfezia Claveryi and Terfezia Boudieri Using Cyclodextrins for Targeted Breast Cancer Therapy |
| title_full | Nanoencapsulation of Terfezia Claveryi and Terfezia Boudieri Using Cyclodextrins for Targeted Breast Cancer Therapy |
| title_fullStr | Nanoencapsulation of Terfezia Claveryi and Terfezia Boudieri Using Cyclodextrins for Targeted Breast Cancer Therapy |
| title_full_unstemmed | Nanoencapsulation of Terfezia Claveryi and Terfezia Boudieri Using Cyclodextrins for Targeted Breast Cancer Therapy |
| title_short | Nanoencapsulation of Terfezia Claveryi and Terfezia Boudieri Using Cyclodextrins for Targeted Breast Cancer Therapy |
| title_sort | nanoencapsulation of terfezia claveryi and terfezia boudieri using cyclodextrins for targeted breast cancer therapy |
| topic | Desert truffles Terfezia claveryi Terfezia boudieri Nanoencapsulation Cyclodextrin- nano-inclusion complexes Breast cancer cytotoxicity Chemicals and Drugs Diseases Medicine and Health Sciences Pharmacy and Pharmaceutical Sciences |
| url | https://fount.aucegypt.edu/etds/2723 https://fount.aucegypt.edu/context/etds/article/3721/viewcontent/Roua_Aref_Nouh_Thesis.pdf |
| work_keys_str_mv | AT nouhrouaaref nanoencapsulationofterfeziaclaveryiandterfeziaboudieriusingcyclodextrinsfortargetedbreastcancertherapy |