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Transmission of SARS‑CoV‑2 in northern Ghana: insights from whole‑genome sequencing
This article is published by Springer and is also available at https://doi.org/10.1007/s00705-021-04986-3
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| Format: | Article |
| Language: | English |
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SPRINGER
2023
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| _version_ | 1867613138371739648 |
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| access_status_str | Open Access |
| author | Sylverken Angelina Augustina El‑Duah Philip Owusu-Dabo Ellis Schneider Julia Owusu Michael.. |
| author_browse | El‑Duah Philip Owusu Michael.. Owusu-Dabo Ellis Schneider Julia Sylverken Angelina Augustina |
| author_facet | Sylverken Angelina Augustina El‑Duah Philip Owusu-Dabo Ellis Schneider Julia Owusu Michael.. |
| author_sort | Sylverken Angelina Augustina |
| collection | Thesis |
| description | This article is published by Springer and is also available at https://doi.org/10.1007/s00705-021-04986-3 |
| format | Article |
| id | oai:ir.knust.edu.gh:123456789/14754 |
| institution | KNUST (Ghana) |
| language | English |
| last_indexed | 2026-06-10T12:31:22.621Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from KNUSTSpace — Kwame Nkrumah University of Science & Technology (Ghana) |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | SPRINGER |
| publisherStr | SPRINGER |
| record_format | dspace |
| source_str | KNUSTSpace — Kwame Nkrumah University of Science & Technology (Ghana) |
| spelling | oai:ir.knust.edu.gh:123456789/14754 Transmission of SARS‑CoV‑2 in northern Ghana: insights from whole‑genome sequencing Sylverken Angelina Augustina El‑Duah Philip Owusu-Dabo Ellis Schneider Julia Owusu Michael.. This article is published by Springer and is also available at https://doi.org/10.1007/s00705-021-04986-3 Following the detection of the frst imported case of COVID-19 in the northern sector of Ghana, we molecularly charac terized and phylogenetically analysed sequences, including three complete genome sequences, of severe acute respiratory syndrome coronavirus 2 obtained from nine patients in Ghana. We performed high-throughput sequencing on nine samples that were found to have a high concentration of viral RNA. We also assessed the potential impact that long-distance transport of samples to testing centres may have on sequencing results. Here, two samples that were similar in terms of viral RNA concentration but were transported from sites that are over 400 km apart were analyzed. All sequences were compared to previous sequences from Ghana and representative sequences from regions where our patients had previously travelled. Three complete genome sequences and another nearly complete genome sequence with 95.6% coverage were obtained. Sequences with coverage in excess of 80% were found to belong to three lineages, namely A, B.1 and B.2. Our sequences clustered in two diferent clades, with the majority falling within a clade composed of sequences from sub-Saharan Africa. Less RNA fragmentation was seen in sample KATH23, which was collected 9 km from the testing site, than in sample TTH6, which was collected and transported over a distance of 400 km to the testing site. The clustering of several sequences from sub-Saharan Africa suggests regional circulation of the viruses in the subregion. Importantly, there may be a need to decentralize testing sites and build more capacity across Africa to boost the sequencing output of the subregion. KNUST 2023-12-11T11:33:33Z 2023-12-11T11:33:33Z 2021 Article Archives of Virology (2021) 166:1385–1393 https://doi.org/10.1007/s00705-021-04986-3 10.1007/s00705-021-04986-3 https://ir.knust.edu.gh/handle/123456789/14754 en application/pdf SPRINGER |
| spellingShingle | Sylverken Angelina Augustina El‑Duah Philip Owusu-Dabo Ellis Schneider Julia Owusu Michael.. Transmission of SARS‑CoV‑2 in northern Ghana: insights from whole‑genome sequencing |
| title | Transmission of SARS‑CoV‑2 in northern Ghana: insights from whole‑genome sequencing |
| title_full | Transmission of SARS‑CoV‑2 in northern Ghana: insights from whole‑genome sequencing |
| title_fullStr | Transmission of SARS‑CoV‑2 in northern Ghana: insights from whole‑genome sequencing |
| title_full_unstemmed | Transmission of SARS‑CoV‑2 in northern Ghana: insights from whole‑genome sequencing |
| title_short | Transmission of SARS‑CoV‑2 in northern Ghana: insights from whole‑genome sequencing |
| title_sort | transmission of sars cov 2 in northern ghana insights from whole genome sequencing |
| url | https://ir.knust.edu.gh/handle/123456789/14754 |
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