Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Comparison of molecular and antibiotic resistance pro®le methods for the population analysis of Bradyrhizobium spp. (TGx) isolates that nodulate the new TGx soybean cultivars in Africa
This article is Published by The Society for Applied Microbiology, 2002
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Society for Applied Microbiology
2023
|
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1869483596824182784 |
|---|---|
| access_status_str | Open Access |
| author | Abaidoo R. C Keyser H.H. Singleton P.W. Borthakur D. |
| author_browse | Abaidoo R. C Borthakur D. Keyser H.H. Singleton P.W. |
| author_facet | Abaidoo R. C Keyser H.H. Singleton P.W. Borthakur D. |
| author_sort | Abaidoo R. C |
| collection | Thesis |
| description | This article is Published by The Society for Applied Microbiology, 2002 |
| format | Article |
| id | oai:ir.knust.edu.gh:123456789/14800 |
| institution | KNUST (Ghana) |
| language | English |
| last_indexed | 2026-07-01T04:01:31.233Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from KNUSTSpace — Kwame Nkrumah University of Science & Technology (Ghana) |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | The Society for Applied Microbiology |
| publisherStr | The Society for Applied Microbiology |
| record_format | dspace |
| source_str | KNUSTSpace — Kwame Nkrumah University of Science & Technology (Ghana) |
| spelling | oai:ir.knust.edu.gh:123456789/14800 Comparison of molecular and antibiotic resistance pro®le methods for the population analysis of Bradyrhizobium spp. (TGx) isolates that nodulate the new TGx soybean cultivars in Africa Abaidoo R. C Keyser H.H. Singleton P.W. Borthakur D. This article is Published by The Society for Applied Microbiology, 2002 Aims: Comparison of molecular and antibiotic resistance pro®le methods to identify an easy method that can differentiate between strains of introduced Bradyrhizobium japonicum and the indigenous Bradyrhizobium spp. (TGx) isolates which nodulate the newly developed TGx soybean cultivars in Africa. Methods and Results: Restriction fragment length polymorphism (RFLP) of 16S rDNA generated by ®ve restriction enzymes, banding patterns in Southern hybridization using nod and nif genes as probes, and resistance patterns of the isolates to nine antibiotics, were used to group 26 Bradyrhizobium spp. (TGx) isolates and four other Bradyrhizobium strains. The clusters of isolates obtained from the four grouping methods were all different, although all methods revealed large genetic diversity among the isolates. Conclusions: Results indicate that the antibiotic resistance pro®le method is as good as the three molecular methods used in this study for phylogenetic grouping of the Bradyrhizobium spp. (TGx) isolates, which may serve as a basis for further characterization of selected isolates from each group. Signi®cance and Impact of the Study: The antibiotic resistance pro®le method can be used as a simple means of assessing genetic variability and grouping of a large number of Bradyrhizobium spp. (TGx) isolates. Representative isolates from each group can then be selected for further characterization. KNUST 2023-12-12T10:34:09Z 2023-12-12T10:34:09Z 2002 Article Journal of Applied Microbiology, 92, 109±117 https://ir.knust.edu.gh/handle/123456789/14800 en application/pdf The Society for Applied Microbiology |
| spellingShingle | Abaidoo R. C Keyser H.H. Singleton P.W. Borthakur D. Comparison of molecular and antibiotic resistance pro®le methods for the population analysis of Bradyrhizobium spp. (TGx) isolates that nodulate the new TGx soybean cultivars in Africa |
| title | Comparison of molecular and antibiotic resistance pro®le methods for the population analysis of Bradyrhizobium spp. (TGx) isolates that nodulate the new TGx soybean cultivars in Africa |
| title_full | Comparison of molecular and antibiotic resistance pro®le methods for the population analysis of Bradyrhizobium spp. (TGx) isolates that nodulate the new TGx soybean cultivars in Africa |
| title_fullStr | Comparison of molecular and antibiotic resistance pro®le methods for the population analysis of Bradyrhizobium spp. (TGx) isolates that nodulate the new TGx soybean cultivars in Africa |
| title_full_unstemmed | Comparison of molecular and antibiotic resistance pro®le methods for the population analysis of Bradyrhizobium spp. (TGx) isolates that nodulate the new TGx soybean cultivars in Africa |
| title_short | Comparison of molecular and antibiotic resistance pro®le methods for the population analysis of Bradyrhizobium spp. (TGx) isolates that nodulate the new TGx soybean cultivars in Africa |
| title_sort | comparison of molecular and antibiotic resistance pro r le methods for the population analysis of bradyrhizobium spp tgx isolates that nodulate the new tgx soybean cultivars in africa |
| url | https://ir.knust.edu.gh/handle/123456789/14800 |
| work_keys_str_mv | AT abaidoorc comparisonofmolecularandantibioticresistanceprolemethodsforthepopulationanalysisofbradyrhizobiumspptgxisolatesthatnodulatethenewtgxsoybeancultivarsinafrica AT keyserhh comparisonofmolecularandantibioticresistanceprolemethodsforthepopulationanalysisofbradyrhizobiumspptgxisolatesthatnodulatethenewtgxsoybeancultivarsinafrica AT singletonpw comparisonofmolecularandantibioticresistanceprolemethodsforthepopulationanalysisofbradyrhizobiumspptgxisolatesthatnodulatethenewtgxsoybeancultivarsinafrica AT borthakurd comparisonofmolecularandantibioticresistanceprolemethodsforthepopulationanalysisofbradyrhizobiumspptgxisolatesthatnodulatethenewtgxsoybeancultivarsinafrica |