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The use of surrogate reference standard in Quantitative HPLC: A case study of Albendazole and Quinine sulphate tablets
A thesis submitted in partial fulfillment of the requirement for the degree of Master of Philosophy (Pharmaceutical Chemistry).
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| Format: | Thesis |
| Language: | English |
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2015
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| _version_ | 1867613138205016065 |
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| access_status_str | Open Access |
| author | Obimpeh, Worlanyo James |
| author_browse | Obimpeh, Worlanyo James |
| author_facet | Obimpeh, Worlanyo James |
| author_sort | Obimpeh, Worlanyo James |
| collection | Thesis |
| description | A thesis submitted in partial fulfillment of the
requirement for the degree of
Master of Philosophy
(Pharmaceutical Chemistry). |
| format | Thesis |
| id | oai:ir.knust.edu.gh:123456789/7166 |
| institution | KNUST (Ghana) |
| language | English |
| last_indexed | 2026-06-10T12:31:22.621Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from KNUSTSpace — Kwame Nkrumah University of Science & Technology (Ghana) |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| record_format | dspace |
| source_str | KNUSTSpace — Kwame Nkrumah University of Science & Technology (Ghana) |
| spelling | oai:ir.knust.edu.gh:123456789/7166 The use of surrogate reference standard in Quantitative HPLC: A case study of Albendazole and Quinine sulphate tablets Obimpeh, Worlanyo James A thesis submitted in partial fulfillment of the requirement for the degree of Master of Philosophy (Pharmaceutical Chemistry). A simple, fast and a sensitive Reverse-phase HPLC method was developed for the quantitative analysis of Albendazole and Quinine Sulphate tablets using Aspirin, Amoxicillin Trihydrate and Metronidazole as surrogate reference standards. For the assay of Albendazole tablets, a C-18 waters 4.6 x 150mm column (stationary phase) and a mobile phase made of Methanol -Water (60:40 V /v) at pH of 5.40±0.02 was used. The analysis was carried out at 245nm and flow rate of 1ml/min. The mean retention time of Albendazole, Aspirin, Amoxicillin Trihydrate and Metronidazole were; 4.21±0.036min, 1.74±0.021min, 2.53±0.018min and 2.28±0.027min respectively. When Aspirin was used as a surrogate reference standard, the constant ‘K’ obtained was 2.297±0.004 and the average percentage content of Albendazole in the Alben, Wormplex 400 and the Zeben tablets after five determinations were; 98.22±0.66%, 101.63±0.587% and 98.82±0.535% respectively. When Amoxicillin Trihydrate was used as a surrogate reference standard, the ‘K’ value obtained was 2.693±0.009 and the average percentage content of Albendazole in the Alben, Wormplex 400 and the Zeben tablets after five determinations were; 99.55±0.675%, 101.02±0.639% and 99.79±0.824% respectively. When Metronidazole was used as a surrogate reference standard, the ‘K’ obtained was 1.827±0.003 and the average percentage content of Albendazole in the Alben, Wormplex 400 and Zebe n tablets were 99.37±0.84, 101.13±0.500 and 99.82±0.577 respectively. A C-18 waters 4.6 x 150mm column, mobile phase combination of Methanol -Water (60:40 V /v) with the pH adjusted to 5.20±0.02 were employed for the analysis of Quinine Sulphate tablets, wavelength of detection was 245nm, and flow rate was 1.5ml/min. Quinine Sulphate, Aspirin, Amoxicillin Trihydrate and Metronidazole had average retention time of v 6.65±0.015min, 2.78±0.03min, 3.02±0.017min and 2.02±0.007 min respectively. When Aspirin was used as a surrogate reference standard, the ‘K’ value obtained was 2.549±0.007 and the percentage content of Quinine Sulphate in the Actavis, Co -Pharma and the Wockhardt tablets were; 98.57±0.89%, 102.74±0.64% and 98.18±0.75% respectively. When Amoxicillin Trihydrate was used as a surrogate reference standard, the ‘K’ value obtained was 3.893±0.008 and the average percentage content of Quinine Sulphate in the Actavis, Co -Pharma and the Wockhardt tablets were 99.99±0.43%, 101.84±0.44% and 97.004±0.62% respectively. When Metronidazole was used as a surrogate reference standard, the ‘K’ value obtained was 1.034±0.006 and the average percentage content of Quinine Sulphate in the Actavis, Co-Pharma and the Wockhardt tablets were 99.87±0.42%, 103.22±0.38% and 100.17±0.399% respectively. The results obtained from the developed method were statistically comparable to results obtained from the standard methods. The developed method showed good precision, was robust and linear within the working range, thus making the deve loped methods very suitable for quantification of Albendazole and Quinine Sulphate tablets. KNUST 2015-04-24T11:34:47Z 2023-04-21T12:12:20Z 2015-04-24T11:34:47Z 2023-04-21T12:12:20Z 2015-04-24 Thesis https://ir.knust.edu.gh/handle/123456789/7166 en application/pdf |
| spellingShingle | Obimpeh, Worlanyo James The use of surrogate reference standard in Quantitative HPLC: A case study of Albendazole and Quinine sulphate tablets |
| title | The use of surrogate reference standard in Quantitative HPLC: A case study of Albendazole and Quinine sulphate tablets |
| title_full | The use of surrogate reference standard in Quantitative HPLC: A case study of Albendazole and Quinine sulphate tablets |
| title_fullStr | The use of surrogate reference standard in Quantitative HPLC: A case study of Albendazole and Quinine sulphate tablets |
| title_full_unstemmed | The use of surrogate reference standard in Quantitative HPLC: A case study of Albendazole and Quinine sulphate tablets |
| title_short | The use of surrogate reference standard in Quantitative HPLC: A case study of Albendazole and Quinine sulphate tablets |
| title_sort | use of surrogate reference standard in quantitative hplc a case study of albendazole and quinine sulphate tablets |
| url | https://ir.knust.edu.gh/handle/123456789/7166 |
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