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Characterisation of the flavin adenine dinucleotide binding region in Myxococcus xanthus protoporphyrinogen oxidase
This dissertation focuses on protoporphyrinogen oxidase (PPOX), the penultimate enzyme in the haem biosynthetic pathway. Partial defects in PPOX result in variegate porphyria, an autosomal dominant disorder. PPOX catalyzes the six electron oxidation of protoporphyrinogen IX to protoporphyrin IX, in...
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| Format: | Thesis |
| Language: | English |
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Department of Medicine
2014
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| _version_ | 1867613189004328960 |
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| access_status_str | Open Access |
| author | Boateng, Mavis O |
| author2 | Meissner, Peter |
| author_browse | Boateng, Mavis O Meissner, Peter |
| author_facet | Meissner, Peter Boateng, Mavis O |
| author_sort | Boateng, Mavis O |
| collection | Thesis |
| description | This dissertation focuses on protoporphyrinogen oxidase (PPOX), the penultimate enzyme in the haem biosynthetic pathway. Partial defects in PPOX result in variegate porphyria, an autosomal dominant disorder. PPOX catalyzes the six electron oxidation of protoporphyrinogen IX to protoporphyrin IX, in the presence of flavin adenine dinucleotide (FAD) and oxygen. FAD is a cofactor, functioning as an intermediate electron acceptor in the catalytic function of PPOX. In this study the FAD binding region in Myxococcus xanthus PPOX was analysed by engineering and characterising a selection of relevant mutants. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/10131 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:11.035Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Medicine |
| publisherStr | Department of Medicine |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/10131 Characterisation of the flavin adenine dinucleotide binding region in Myxococcus xanthus protoporphyrinogen oxidase Boateng, Mavis O Meissner, Peter Corrigall, Anne Medicine This dissertation focuses on protoporphyrinogen oxidase (PPOX), the penultimate enzyme in the haem biosynthetic pathway. Partial defects in PPOX result in variegate porphyria, an autosomal dominant disorder. PPOX catalyzes the six electron oxidation of protoporphyrinogen IX to protoporphyrin IX, in the presence of flavin adenine dinucleotide (FAD) and oxygen. FAD is a cofactor, functioning as an intermediate electron acceptor in the catalytic function of PPOX. In this study the FAD binding region in Myxococcus xanthus PPOX was analysed by engineering and characterising a selection of relevant mutants. 2014-12-26T14:17:56Z 2014-12-26T14:17:56Z 2011 Master Thesis Masters MSc http://hdl.handle.net/11427/10131 eng application/pdf Department of Medicine Faculty of Health Sciences University of Cape Town |
| spellingShingle | Medicine Boateng, Mavis O Characterisation of the flavin adenine dinucleotide binding region in Myxococcus xanthus protoporphyrinogen oxidase |
| thesis_degree_str | Master's |
| title | Characterisation of the flavin adenine dinucleotide binding region in Myxococcus xanthus protoporphyrinogen oxidase |
| title_full | Characterisation of the flavin adenine dinucleotide binding region in Myxococcus xanthus protoporphyrinogen oxidase |
| title_fullStr | Characterisation of the flavin adenine dinucleotide binding region in Myxococcus xanthus protoporphyrinogen oxidase |
| title_full_unstemmed | Characterisation of the flavin adenine dinucleotide binding region in Myxococcus xanthus protoporphyrinogen oxidase |
| title_short | Characterisation of the flavin adenine dinucleotide binding region in Myxococcus xanthus protoporphyrinogen oxidase |
| title_sort | characterisation of the flavin adenine dinucleotide binding region in myxococcus xanthus protoporphyrinogen oxidase |
| topic | Medicine |
| url | http://hdl.handle.net/11427/10131 |
| work_keys_str_mv | AT boatengmaviso characterisationoftheflavinadeninedinucleotidebindingregioninmyxococcusxanthusprotoporphyrinogenoxidase |