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Catalysis, substrate binding and specificity in the amidase from Nesterenkonia species
To investigate the structural determinants of NitN specificity on short aliphatic amide substrates by analyzing binding and interactions of these molecules with the NitN binding pocket. To probe the catalytic role of the two active site glutamate residues (Glu61 and Glu139) using NitN as a model enz...
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2015
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| _version_ | 1867613191141326848 |
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| access_status_str | Open Access |
| author | Kimani, Serah |
| author2 | Sewell, Trevor |
| author_browse | Kimani, Serah Sewell, Trevor |
| author_facet | Sewell, Trevor Kimani, Serah |
| author_sort | Kimani, Serah |
| collection | Thesis |
| description | To investigate the structural determinants of NitN specificity on short aliphatic amide substrates by analyzing binding and interactions of these molecules with the NitN binding pocket. To probe the catalytic role of the two active site glutamate residues (Glu61 and Glu139) using NitN as a model enzyme. To monitor the activity, interactions and reactivity of the WT NitN and the Glu61 and Glu139 NitN mutants with ACR. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/10837 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:13.078Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/10837 Catalysis, substrate binding and specificity in the amidase from Nesterenkonia species Kimani, Serah Sewell, Trevor Cell Biology To investigate the structural determinants of NitN specificity on short aliphatic amide substrates by analyzing binding and interactions of these molecules with the NitN binding pocket. To probe the catalytic role of the two active site glutamate residues (Glu61 and Glu139) using NitN as a model enzyme. To monitor the activity, interactions and reactivity of the WT NitN and the Glu61 and Glu139 NitN mutants with ACR. 2015-01-01T12:38:51Z 2015-01-01T12:38:51Z 2011 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/10837 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Cell Biology Kimani, Serah Catalysis, substrate binding and specificity in the amidase from Nesterenkonia species |
| thesis_degree_str | Doctoral |
| title | Catalysis, substrate binding and specificity in the amidase from Nesterenkonia species |
| title_full | Catalysis, substrate binding and specificity in the amidase from Nesterenkonia species |
| title_fullStr | Catalysis, substrate binding and specificity in the amidase from Nesterenkonia species |
| title_full_unstemmed | Catalysis, substrate binding and specificity in the amidase from Nesterenkonia species |
| title_short | Catalysis, substrate binding and specificity in the amidase from Nesterenkonia species |
| title_sort | catalysis substrate binding and specificity in the amidase from nesterenkonia species |
| topic | Cell Biology |
| url | http://hdl.handle.net/11427/10837 |
| work_keys_str_mv | AT kimaniserah catalysissubstratebindingandspecificityintheamidasefromnesterenkoniaspecies |