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The isolation and characterization of heat shock protein Hsp12 in Lipomyces starkeyi
Bibliography: leaves 60-72.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2015
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| _version_ | 1867613257199517696 |
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| access_status_str | Open Access |
| author | Mukwevho, Emmanuel |
| author2 | Brandt, Wolf F |
| author_browse | Brandt, Wolf F Mukwevho, Emmanuel |
| author_facet | Brandt, Wolf F Mukwevho, Emmanuel |
| author_sort | Mukwevho, Emmanuel |
| collection | Thesis |
| description | Bibliography: leaves 60-72. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/10953 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:15.376Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/10953 The isolation and characterization of heat shock protein Hsp12 in Lipomyces starkeyi Mukwevho, Emmanuel Brandt, Wolf F Lindsey, George G Molecular and Cellular Biology Bibliography: leaves 60-72. The stress response protein Hsp 12 is induced in S. cerevisiae cells upon exposure to salt stress, heat shock, ethanol, and upon entry to stationary phase (Mtwisha et aI., 1998). In this study, the occurrence of proteins related to Hsp12 was investigated in a number of yeasts (namely, Saccharomyces cerevisiae S288C, Schizosaccharomyces pombe, Debaromyces hansenii, Lipomyces starkeyi Y-2024, Saccharomyces cerevisiae IFO 23X7 (Kaokai), Zygosaccharomyces rouxii and Pichia sorbitophila. This was performed by selective protein extraction followed by SDS-P AGE and western blotting using a S. cerevisiae anti-Hsp 12 antibody. The results showed that almost all the yeasts investigated possessed a protein that had an identical migration to that of Hsp 12 with the exception of S. pombe, which contained a 9 kDa protein. Western blotting using the antiHsp 12 antibody cross-reacted only with the two S. cerevisiae species in addition to the 12 kDa protein from Lipomyces starkeyi of all the species investigated. MALDI-TOF peptide mass analysis after tryptic digestion of the L. starkeyi 12 kDa protein showed that a close sequence similarity existed to that of S. cerevisiae Hsp 12 and none to rest of the 12 kDa proteins isolated from all the other species investigated. In order to determine the sequence of the Hsp 12 protein, the L. starkeyi Hsp 12 gene was amplified using S. cerevisiae Hsp 12 primers. Gene sequencing of both S. cerevisiae and L. starkeyi Hsp 12 genes revealed three nucleotide differences existed between them. L. starkeyi Hsp 12 was found to be present in relatively small amounts during early growth stages but increased during log phase with a slight further increase during stationary phase. Increasing the salt concentration in the growth medium was found to induce Hsp 12. Increased levels of Hsp 12 appeared to confer a degree of protection during desiccation and subsequent rehydration of both L. starkeyi and S. cerevisiae. 2015-01-02T09:04:17Z 2015-01-02T09:04:17Z 2002 Master Thesis Masters MSc http://hdl.handle.net/11427/10953 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Molecular and Cellular Biology Mukwevho, Emmanuel The isolation and characterization of heat shock protein Hsp12 in Lipomyces starkeyi |
| thesis_degree_str | Master's |
| title | The isolation and characterization of heat shock protein Hsp12 in Lipomyces starkeyi |
| title_full | The isolation and characterization of heat shock protein Hsp12 in Lipomyces starkeyi |
| title_fullStr | The isolation and characterization of heat shock protein Hsp12 in Lipomyces starkeyi |
| title_full_unstemmed | The isolation and characterization of heat shock protein Hsp12 in Lipomyces starkeyi |
| title_short | The isolation and characterization of heat shock protein Hsp12 in Lipomyces starkeyi |
| title_sort | isolation and characterization of heat shock protein hsp12 in lipomyces starkeyi |
| topic | Molecular and Cellular Biology |
| url | http://hdl.handle.net/11427/10953 |
| work_keys_str_mv | AT mukwevhoemmanuel theisolationandcharacterizationofheatshockproteinhsp12inlipomycesstarkeyi AT mukwevhoemmanuel isolationandcharacterizationofheatshockproteinhsp12inlipomycesstarkeyi |