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Immunochemical studies with tryptic peptides of tobacco mosaic virus protein
Includes bibliographical references.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2015
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| _version_ | 1867613337432358913 |
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| access_status_str | Open Access |
| author | Milton, Raymond Cecil de Lisle |
| author2 | Van Regenmortel, M H V |
| author_browse | Milton, Raymond Cecil de Lisle Van Regenmortel, M H V |
| author_facet | Van Regenmortel, M H V Milton, Raymond Cecil de Lisle |
| author_sort | Milton, Raymond Cecil de Lisle |
| collection | Thesis |
| description | Includes bibliographical references. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/12596 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:32.198Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/12596 Immunochemical studies with tryptic peptides of tobacco mosaic virus protein Milton, Raymond Cecil de Lisle Van Regenmortel, M H V Biochemistry Includes bibliographical references. The antigenic determinants of the protein subunit of tobacco mosaic virus CTMV) have been studied by inhibition of complement fixation, inhibition of micro-precipitin and direct binding experiments. The viral subunit has been found to possess six antigenic determinants. Two of these, situated in tryptic peptide I (residues 1-41), were found to be a cryptotope (i.e. an antigenic determinant absent from the outer surface of the assembled viral capsid) and a viral antigenic determinant. Tryptic peptides 4 (residues 62-68) and 8(residues 93-112) also contained cryptotopes which were situated in the region of residues 63-65 and 108-112 respectively. Tryptic peptide 12 (residues 142-158) contained both a cryptotope and a neotope (i.e. an antigenic determinant which is only expressed on the outer surface of the viral capsid), which are situated in the C-terminal region of the polypeptide chain of the TMV protein, residue156 being associated with the cryptotope and residue 158 with the neotope. No antigenic activity could be demonstrated in tryptic peptide I I (residues 135-141 ). When the results are analyzed in terms of the three-dimensional structure of the viral subunit, it appears that all the antigenic reactive regions occupy highly accessible locations on the surface of the protein. 2015-03-13T14:06:24Z 2015-03-13T14:06:24Z 1979 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/12596 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Biochemistry Milton, Raymond Cecil de Lisle Immunochemical studies with tryptic peptides of tobacco mosaic virus protein |
| thesis_degree_str | Doctoral |
| title | Immunochemical studies with tryptic peptides of tobacco mosaic virus protein |
| title_full | Immunochemical studies with tryptic peptides of tobacco mosaic virus protein |
| title_fullStr | Immunochemical studies with tryptic peptides of tobacco mosaic virus protein |
| title_full_unstemmed | Immunochemical studies with tryptic peptides of tobacco mosaic virus protein |
| title_short | Immunochemical studies with tryptic peptides of tobacco mosaic virus protein |
| title_sort | immunochemical studies with tryptic peptides of tobacco mosaic virus protein |
| topic | Biochemistry |
| url | http://hdl.handle.net/11427/12596 |
| work_keys_str_mv | AT miltonraymondcecildelisle immunochemicalstudieswithtrypticpeptidesoftobaccomosaicvirusprotein |