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Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains
Includes bibliographical references.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Molecular and Cell Biology
2015
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| _version_ | 1867613805124517888 |
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| access_status_str | Open Access |
| author | Reeve, Byron William Patrick |
| author2 | Reid, Sharon J |
| author_browse | Reeve, Byron William Patrick Reid, Sharon J |
| author_facet | Reid, Sharon J Reeve, Byron William Patrick |
| author_sort | Reeve, Byron William Patrick |
| collection | Thesis |
| description | Includes bibliographical references. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/12946 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:41:58.913Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/12946 Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains Reeve, Byron William Patrick Reid, Sharon J Molecular and Cell Biology Includes bibliographical references. The acetone- butanol-ethanol (ABE) fermentation was one of the first fermentation processes to be industrialized on a large scale, and the dominant product, butanol is particularly significant due to its potential as a modern day fuel additive or fuel extender in the petrochemical industry. A collection of 19 solventogenic Clostridium beijerinckii and 11 Clostridium saccharobutylicum strains isolated from the National Chemical Products (NCP) ABE fermentation plant in Germiston, South Africa, were classed according to species by a quick species-specific colony PCR and by rifampicin screening methods respectively. The speciesspecific PCR aims to provide a rapid means of assessing any contamination of an ABE batch fermentation by differentiating between C. saccharobutylicum and C. beijerinckii species. Random Amplification of Polymorphic DNA (RAPD) analysis generated four C. beijerinckii and two C. saccharobutylicum strain groups respectively. Multilocus Sequence Typing (MLST) was developed for a smaller selection of strains and showed a further two strain groups within the NCP C. beijerinckii strains and three groups within the C. saccharobutylicum strains. 2015-05-28T04:11:15Z 2015-05-28T04:11:15Z 2014 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/12946 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Molecular and Cell Biology Reeve, Byron William Patrick Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains |
| thesis_degree_str | Doctoral |
| title | Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains |
| title_full | Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains |
| title_fullStr | Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains |
| title_full_unstemmed | Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains |
| title_short | Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains |
| title_sort | nitrogen metabolism and butanol production by south african clostridium beijerinckii and clostridium saccharobutylicum strains |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/12946 |
| work_keys_str_mv | AT reevebyronwilliampatrick nitrogenmetabolismandbutanolproductionbysouthafricanclostridiumbeijerinckiiandclostridiumsaccharobutylicumstrains |