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Evaluation of the P13K pathway and downstream effect in Her-2 positive and negative breast carcinomas
Breast cancer management continues to be a challenge due to the heterogeneity of the disease and the fact that individuals with the same stage and pathological diagnosis may respond differently to treatment as a result of differences in gene expression. Several components of the Her-2/PI3K/Akt pathw...
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| Format: | Thesis |
| Language: | English |
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Division of Anatomical Pathology
2015
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| _version_ | 1867613165843382272 |
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| access_status_str | Open Access |
| author | Rademan, Anina |
| author2 | Govender, Dhiren |
| author_browse | Govender, Dhiren Rademan, Anina |
| author_facet | Govender, Dhiren Rademan, Anina |
| author_sort | Rademan, Anina |
| collection | Thesis |
| description | Breast cancer management continues to be a challenge due to the heterogeneity of the disease and the fact that individuals with the same stage and pathological diagnosis may respond differently to treatment as a result of differences in gene expression. Several components of the Her-2/PI3K/Akt pathway were evaluated for their relevance as potential prognostic markers or indicators for treatment. A secondary objective was to evaluate the CISH technique for its suitability for analysis of Her-2 gene amplification on archived, Papanicolaou-stained fine needle aspiration (FNA) samples. Tissue blocks from a retrospective series of 93 primary breast carcinoma cases were selected, based on their Her-2 status. Twenty six of these cases received trastuzumab treatment while 67 did not. Expression of Her-2, ER, PI3K, PTEN, p-Akt, BCL2, NFκB, MDM2 and p53 were analysed and compared with various clinicopathological features. The CISH technique was evaluated for its suitability for analysis of Her-2 gene amplification on archived, Papanicolaou-stained FNA samples. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/13965 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:31:48.735Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Division of Anatomical Pathology |
| publisherStr | Division of Anatomical Pathology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/13965 Evaluation of the P13K pathway and downstream effect in Her-2 positive and negative breast carcinomas Rademan, Anina Govender, Dhiren Anatomical Pathology Breast cancer management continues to be a challenge due to the heterogeneity of the disease and the fact that individuals with the same stage and pathological diagnosis may respond differently to treatment as a result of differences in gene expression. Several components of the Her-2/PI3K/Akt pathway were evaluated for their relevance as potential prognostic markers or indicators for treatment. A secondary objective was to evaluate the CISH technique for its suitability for analysis of Her-2 gene amplification on archived, Papanicolaou-stained fine needle aspiration (FNA) samples. Tissue blocks from a retrospective series of 93 primary breast carcinoma cases were selected, based on their Her-2 status. Twenty six of these cases received trastuzumab treatment while 67 did not. Expression of Her-2, ER, PI3K, PTEN, p-Akt, BCL2, NFκB, MDM2 and p53 were analysed and compared with various clinicopathological features. The CISH technique was evaluated for its suitability for analysis of Her-2 gene amplification on archived, Papanicolaou-stained FNA samples. 2015-09-15T10:22:33Z 2015-09-15T10:22:33Z 2014 Master Thesis Masters MSc (Med) http://hdl.handle.net/11427/13965 eng application/pdf Division of Anatomical Pathology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Anatomical Pathology Rademan, Anina Evaluation of the P13K pathway and downstream effect in Her-2 positive and negative breast carcinomas |
| thesis_degree_str | Master's |
| title | Evaluation of the P13K pathway and downstream effect in Her-2 positive and negative breast carcinomas |
| title_full | Evaluation of the P13K pathway and downstream effect in Her-2 positive and negative breast carcinomas |
| title_fullStr | Evaluation of the P13K pathway and downstream effect in Her-2 positive and negative breast carcinomas |
| title_full_unstemmed | Evaluation of the P13K pathway and downstream effect in Her-2 positive and negative breast carcinomas |
| title_short | Evaluation of the P13K pathway and downstream effect in Her-2 positive and negative breast carcinomas |
| title_sort | evaluation of the p13k pathway and downstream effect in her 2 positive and negative breast carcinomas |
| topic | Anatomical Pathology |
| url | http://hdl.handle.net/11427/13965 |
| work_keys_str_mv | AT rademananina evaluationofthep13kpathwayanddownstreameffectinher2positiveandnegativebreastcarcinomas |