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Histone modifications and the Arabidopsis thaliana circadian clock
Includes bibliographical references (leaves 61-84).
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2015
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| _version_ | 1867613357683507200 |
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| access_status_str | Open Access |
| author | Motleleng, Liabo Lilian |
| author2 | Roden, Laura |
| author_browse | Motleleng, Liabo Lilian Roden, Laura |
| author_facet | Roden, Laura Motleleng, Liabo Lilian |
| author_sort | Motleleng, Liabo Lilian |
| collection | Thesis |
| description | Includes bibliographical references (leaves 61-84). |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/14719 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:52.199Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/14719 Histone modifications and the Arabidopsis thaliana circadian clock Motleleng, Liabo Lilian Roden, Laura Molecular and Cell Biology Includes bibliographical references (leaves 61-84). The circadian system has a regulatory role in almost all aspects of a plant's life. In Arabidopsis thaliana, almost 36% of the genome has been shown to be circadianly regulated and many genes that are circadianly regulated have been shown to be light responsive or involved in light responses. Rhythmic histone acetylation has been demonstrated in the promoter of TIMING OF CAB EXPRESSION1 (TOC1). Here, I used semi-quantitative Reverse Transcriptase Polymerase Chain Reaction (semi-quantitative RT -PCR) to investigate which enzymes are involved in the rhythmic expression of TOC1. I also determined whether loss-of-function histone acetylation and methylation mutants could affect the overall functioning of the circadian oscillator by measuring their circadian leaf movement and delayed fluorescence (DF) rhythms. GCN5/ HAG1 mutant plants (gcn5) exhibited erratic TOC1 expression in both constant dark (DD) and constant light (LL) conditions. Although TOC1 expression appeared to be rhythmic in both DD and LL conditions, the waveform of the rhythm was altered in TATA-binding protein associated factor 1 (taf1) mutants. This suggested that TAF1 and GCN5 might play different roles in the rhythmic histone acetylation affecting TOC1 expression. DF data and leaf movement data indicated that both TAF1 and GCN5 might play a role in the overall functioning of the A. thaliana circadian clock. Arrhythmic TOC1 expression and DF was observed in histone deacetylase 1 (hd1) mutants, suggesting that HD1 is not only involved in the rhythmic histone deacetylation affecting TOC1 expression but in the overall functioning of the circadian clock. Semi-quantitative RTPCR, DF and leaf movement studies demonstrated that CURLY LEAF (CLF), a histone methylase is involved in both the histone methylation affecting TOC1 expression and in the overall functioning of the A. thaliana circadian clock. 2015-11-08T04:59:07Z 2015-11-08T04:59:07Z 2010 Master Thesis Masters MSc http://hdl.handle.net/11427/14719 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Molecular and Cell Biology Motleleng, Liabo Lilian Histone modifications and the Arabidopsis thaliana circadian clock |
| thesis_degree_str | Master's |
| title | Histone modifications and the Arabidopsis thaliana circadian clock |
| title_full | Histone modifications and the Arabidopsis thaliana circadian clock |
| title_fullStr | Histone modifications and the Arabidopsis thaliana circadian clock |
| title_full_unstemmed | Histone modifications and the Arabidopsis thaliana circadian clock |
| title_short | Histone modifications and the Arabidopsis thaliana circadian clock |
| title_sort | histone modifications and the arabidopsis thaliana circadian clock |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/14719 |
| work_keys_str_mv | AT motlelengliabolilian histonemodificationsandthearabidopsisthalianacircadianclock |