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Glutamine synthetase in Bacteroides fragilis
Includes bibliographical references (p. 78-90).
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2015
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| _version_ | 1867613621670903808 |
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| access_status_str | Open Access |
| author | Tumba, Nancy |
| author2 | Abratt, Valerie Rose |
| author_browse | Abratt, Valerie Rose Tumba, Nancy |
| author_facet | Abratt, Valerie Rose Tumba, Nancy |
| author_sort | Tumba, Nancy |
| collection | Thesis |
| description | Includes bibliographical references (p. 78-90). |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/14720 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:39:03.958Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/14720 Glutamine synthetase in Bacteroides fragilis Tumba, Nancy Abratt, Valerie Rose Molecular and Cell Biology Includes bibliographical references (p. 78-90). Bactereroides fragilis is a gram-negative, non spore-forming, obligate anaerobe of the human intestinal microbiota. It is, however, an opportunistic pathogen and has been ranked as the most prevalent isolate in cases of anaerobic septicaemia. Similar to most bacteria, ammonium is assimilated in B. fragilis through the action of glutamine synthetase (GS). Glutamine is vital to nitrogen metabolism as it serves as a precursor to many secondary metabolites. GS enzymes are, therefore, vital to the growth of the organism and many prokaryotes are known to possess two or more isoforms of the enzyme. In addition, GS expression and regulation is usually tightly regulated in concert with the availability of nitrogen. Previous studies have identified a single GSIII encoding gene (glnN) in B. fragilis. In this dissertation, an additional ORF coding for a putative GSI enzyme in B. fragilis was identified, isolated and functionally characterized. A putative regulatory protein was also identified and its functional contribution to nitrogen metabolism was determined, in order to extend our understanding of nitrogen assimilation in B. fragilis. 2015-11-08T04:59:11Z 2015-11-08T04:59:11Z 2007 Master Thesis Masters MSc http://hdl.handle.net/11427/14720 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Molecular and Cell Biology Tumba, Nancy Glutamine synthetase in Bacteroides fragilis |
| thesis_degree_str | Master's |
| title | Glutamine synthetase in Bacteroides fragilis |
| title_full | Glutamine synthetase in Bacteroides fragilis |
| title_fullStr | Glutamine synthetase in Bacteroides fragilis |
| title_full_unstemmed | Glutamine synthetase in Bacteroides fragilis |
| title_short | Glutamine synthetase in Bacteroides fragilis |
| title_sort | glutamine synthetase in bacteroides fragilis |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/14720 |
| work_keys_str_mv | AT tumbanancy glutaminesynthetaseinbacteroidesfragilis |