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A proteomic investigation of the immune response of the South African abalone, Haliotis midae
Includes bibliographical references
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of English Language and Literature
2016
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| _version_ | 1867613212333047808 |
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| access_status_str | Open Access |
| author | Beltran, Caroline Gina Gracieuse |
| author2 | Coyne, Vernon |
| author_browse | Beltran, Caroline Gina Gracieuse Coyne, Vernon |
| author_facet | Coyne, Vernon Beltran, Caroline Gina Gracieuse |
| author_sort | Beltran, Caroline Gina Gracieuse |
| collection | Thesis |
| description | Includes bibliographical references |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/16483 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:33.381Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of English Language and Literature |
| publisherStr | Department of English Language and Literature |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/16483 A proteomic investigation of the immune response of the South African abalone, Haliotis midae Beltran, Caroline Gina Gracieuse Coyne, Vernon Molecular and Cell Biology Includes bibliographical references Haliotis midae is a commercially important abalone in South Africa, previously harvested from a stable, quota-managed fishery. However, the combined effects of overharvesting, increased illegal catches and negative environmental factors led to a collapse in wild populations in the mid-90s. Consequently, land-based aquaculture of H. midae has grown significantly in South Africa, to satisfy the global demand for abalone and alleviate pressure on wild stocks. Unfortunately, disease outbreaks have had a severe impact on the abalone aquaculture industry internationally and remain one of the single biggest factors contributing to economic loss. Understanding the effects of pathogen infection of abalone is therefore crucial to mitigating and controlling infection outbreaks on farms. Despite this, the molecular mechanisms underlying the immune response of H. midae remain obscure. High-throughput proteomics, a powerful tool to analyse global protein expression changes, can provide an integrated view of the immune system. Thus, this study aimed to elucidate the haemocyte proteome of H. midae and gain insight into regulatory molecular pathways underlying innate immunity. In this study, a comparative shotgun proteomics approach using isobaric tagging for relative and absolute quantification (iTRAQ) coupled with LC-MS/MS was employed to investigate H. midae proteome changes in response to Vibrio anguillarum challenge. A preliminary iTRAQ challenge trial was conducted which identified a putative early (1 and 2 hours post-injection) and late (48 hours post-injection) proteome response to bacterial-challenge. Using these time points, four independent challenge trials were conducted and analysed by iTRAQ and the results combined to produce a high-confidence dataset with good quantitative reproducibility for statistical analysis. A parallel set of experiments was conducted using mock-infected samples. 2016-01-21T11:12:39Z 2016-01-21T11:12:39Z 2015 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/16483 eng application/pdf Department of English Language and Literature Faculty of Humanities University of Cape Town |
| spellingShingle | Molecular and Cell Biology Beltran, Caroline Gina Gracieuse A proteomic investigation of the immune response of the South African abalone, Haliotis midae |
| thesis_degree_str | Doctoral |
| title | A proteomic investigation of the immune response of the South African abalone, Haliotis midae |
| title_full | A proteomic investigation of the immune response of the South African abalone, Haliotis midae |
| title_fullStr | A proteomic investigation of the immune response of the South African abalone, Haliotis midae |
| title_full_unstemmed | A proteomic investigation of the immune response of the South African abalone, Haliotis midae |
| title_short | A proteomic investigation of the immune response of the South African abalone, Haliotis midae |
| title_sort | proteomic investigation of the immune response of the south african abalone haliotis midae |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/16483 |
| work_keys_str_mv | AT beltrancarolineginagracieuse aproteomicinvestigationoftheimmuneresponseofthesouthafricanabalonehaliotismidae AT beltrancarolineginagracieuse proteomicinvestigationoftheimmuneresponseofthesouthafricanabalonehaliotismidae |