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The analysis of marine algae photosynthetic pigments by high performance liquid chromatography
Bibliography: pages 157-165.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Chemistry
2016
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| _version_ | 1867613278773968896 |
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| access_status_str | Open Access |
| author | Bacon, Elizabeth Jane |
| author2 | Orren, M J |
| author_browse | Bacon, Elizabeth Jane Orren, M J |
| author_facet | Orren, M J Bacon, Elizabeth Jane |
| author_sort | Bacon, Elizabeth Jane |
| collection | Thesis |
| description | Bibliography: pages 157-165. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/17047 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:35.758Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Chemistry |
| publisherStr | Department of Chemistry |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/17047 The analysis of marine algae photosynthetic pigments by high performance liquid chromatography Bacon, Elizabeth Jane Orren, M J Analytical Science Bibliography: pages 157-165. A method of analysis of the photosynthetic pigments of in vitro algal cultures, using reverse-phase high performance liquid chromatography (HPLC) is described. This technique was extended to analysis of photosynthetic pigments in samples from the SIBEX-I Expedition; a preconcentration technique was developed to allow a reduction in sample volume for waters of low productivity. HPLC analysis allows accurate chlorophyll a determinations to be made in the presence of the spectrally similar chlorophyllide a, which has been found to be the major chlorophyll a breakdown product present in natural waters. Comparative studies with the SCOR-UNESCO method have shown that as a result of chlorophyllide interference the chlorophyll a concentrations are in fact over-estimates to varying degrees depending on the productivity of the biomass. In addition, quantification of chlorophylls b and c and the carotenoid complement allows identification of the major algal species present, and provides an indication of the physiological status of the bloom. 2016-02-15T07:16:06Z 2016-02-15T07:16:06Z 1984 Master Thesis Masters MSc http://hdl.handle.net/11427/17047 eng application/pdf Department of Chemistry Faculty of Science University of Cape Town |
| spellingShingle | Analytical Science Bacon, Elizabeth Jane The analysis of marine algae photosynthetic pigments by high performance liquid chromatography |
| thesis_degree_str | Master's |
| title | The analysis of marine algae photosynthetic pigments by high performance liquid chromatography |
| title_full | The analysis of marine algae photosynthetic pigments by high performance liquid chromatography |
| title_fullStr | The analysis of marine algae photosynthetic pigments by high performance liquid chromatography |
| title_full_unstemmed | The analysis of marine algae photosynthetic pigments by high performance liquid chromatography |
| title_short | The analysis of marine algae photosynthetic pigments by high performance liquid chromatography |
| title_sort | analysis of marine algae photosynthetic pigments by high performance liquid chromatography |
| topic | Analytical Science |
| url | http://hdl.handle.net/11427/17047 |
| work_keys_str_mv | AT baconelizabethjane theanalysisofmarinealgaephotosyntheticpigmentsbyhighperformanceliquidchromatography AT baconelizabethjane analysisofmarinealgaephotosyntheticpigmentsbyhighperformanceliquidchromatography |