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Studies on bacteria able to use methane in denitrification
The aim of this investigation was to determine the suitability of single carbon organic compounds as hydrogen donors for denitrification. Although methanol has been used for this purpose with some success, methane has apparently been disregarded as a possible carbon source. A denitrifying unit was o...
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867613233873944576 |
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| access_status_str | Open Access |
| author | Davies, Trevor Rodney |
| author2 | Lütjeharms, W J |
| author_browse | Davies, Trevor Rodney Lütjeharms, W J |
| author_facet | Lütjeharms, W J Davies, Trevor Rodney |
| author_sort | Davies, Trevor Rodney |
| collection | Thesis |
| description | The aim of this investigation was to determine the suitability of single carbon organic compounds as hydrogen donors for denitrification. Although methanol has been used for this purpose with some success, methane has apparently been disregarded as a possible carbon source. A denitrifying unit was operated with methanol as a carbon source, in order to determine the influence of methanol on rates of denitrification with several different nitrate concentrations. With methanol as an additive, nitrate reduction was of the order of 90%, while less than 40% denitrification was achieved with the supernatant from settled domestic sewage as the sole carbon source. Isolates were taken from this unit for identification. A similar unit was constructed, receiving methane as the sole carbon source. Bacteria were isolated from this unit and shown to be able to use nitrate as a terminal electron acceptor and as a source of cell nitrogen. These bacteria were identified and their specificity to methane tested. In addition to methane they were found to be able to use several other organic compounds for growth and denitrification. Isolates from the methanol unit were found to be able to use methane for denitrification. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/17700 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:52.713Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/17700 Studies on bacteria able to use methane in denitrification Davies, Trevor Rodney Lütjeharms, W J Microbiology The aim of this investigation was to determine the suitability of single carbon organic compounds as hydrogen donors for denitrification. Although methanol has been used for this purpose with some success, methane has apparently been disregarded as a possible carbon source. A denitrifying unit was operated with methanol as a carbon source, in order to determine the influence of methanol on rates of denitrification with several different nitrate concentrations. With methanol as an additive, nitrate reduction was of the order of 90%, while less than 40% denitrification was achieved with the supernatant from settled domestic sewage as the sole carbon source. Isolates were taken from this unit for identification. A similar unit was constructed, receiving methane as the sole carbon source. Bacteria were isolated from this unit and shown to be able to use nitrate as a terminal electron acceptor and as a source of cell nitrogen. These bacteria were identified and their specificity to methane tested. In addition to methane they were found to be able to use several other organic compounds for growth and denitrification. Isolates from the methanol unit were found to be able to use methane for denitrification. 2016-03-14T07:14:47Z 2016-03-14T07:14:47Z 1973 Master Thesis Masters MSc http://hdl.handle.net/11427/17700 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Microbiology Davies, Trevor Rodney Studies on bacteria able to use methane in denitrification |
| thesis_degree_str | Master's |
| title | Studies on bacteria able to use methane in denitrification |
| title_full | Studies on bacteria able to use methane in denitrification |
| title_fullStr | Studies on bacteria able to use methane in denitrification |
| title_full_unstemmed | Studies on bacteria able to use methane in denitrification |
| title_short | Studies on bacteria able to use methane in denitrification |
| title_sort | studies on bacteria able to use methane in denitrification |
| topic | Microbiology |
| url | http://hdl.handle.net/11427/17700 |
| work_keys_str_mv | AT daviestrevorrodney studiesonbacteriaabletousemethaneindenitrification |