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An electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris plana
Includes bibliographical references.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
MRC/UCT RU for Exercise and Sport Medicine
2016
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| _version_ | 1867614492923265024 |
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| access_status_str | Open Access |
| author | Fisher, David Willis |
| author2 | Isaacson, Leon |
| author_browse | Fisher, David Willis Isaacson, Leon |
| author_facet | Isaacson, Leon Fisher, David Willis |
| author_sort | Fisher, David Willis |
| collection | Thesis |
| description | Includes bibliographical references. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/17861 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:52:54.849Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | MRC/UCT RU for Exercise and Sport Medicine |
| publisherStr | MRC/UCT RU for Exercise and Sport Medicine |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/17861 An electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris plana Fisher, David Willis Isaacson, Leon Physiology Includes bibliographical references. A knowledge of the ionic and electrical gradients across the Malpighian tubules of Onymacris plana is fundamental to the understanding of the transport mechanisms of fluid across the basolateral and apical membranes. Until now no one has investigated the isolated perfused Malpighian tubule of Onymacris plana and simultaneously measured basolateral potentials. Malpighian tubule segments (about lmm length) were dissected out and perfused in vitro according to the method of Burg et. al. (1966), while the basolateral membrane potential (Vbl) was measured by means of an intracellular microelectrode. Using cable analysis, the effects of DNP, chloride free Ringers, BaCl² (a K⁺-channel blocker), low (25 mM) and high (130 mM) pntassium Ringers (bathing medium), cyclic-AMP and a corpora cardiaca homogenate (CCH) on. the transepithelial resistance and the fractional resistances of the basolateral and apical membranes were investigated. 2016-03-17T07:06:21Z 2016-03-17T07:06:21Z 1993 Master Thesis Masters MSc http://hdl.handle.net/11427/17861 eng application/pdf MRC/UCT RU for Exercise and Sport Medicine Faculty of Health Sciences University of Cape Town |
| spellingShingle | Physiology Fisher, David Willis An electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris plana |
| thesis_degree_str | Master's |
| title | An electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris plana |
| title_full | An electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris plana |
| title_fullStr | An electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris plana |
| title_full_unstemmed | An electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris plana |
| title_short | An electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris plana |
| title_sort | electrophysiological study of ion transport across the isolated perfused malpighian tubule of onymacris plana |
| topic | Physiology |
| url | http://hdl.handle.net/11427/17861 |
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