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The binding of divalent cations to tobacco mosaic virus and to some isometric plant viruses
Bibliography: pages 202-212.
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867614241370931200 |
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| access_status_str | Open Access |
| author | Hendry, Donald Arthur |
| author2 | Durham, Tony |
| author_browse | Durham, Tony Hendry, Donald Arthur |
| author_facet | Durham, Tony Hendry, Donald Arthur |
| author_sort | Hendry, Donald Arthur |
| collection | Thesis |
| description | Bibliography: pages 202-212. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/17879 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:48:54.950Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/17879 The binding of divalent cations to tobacco mosaic virus and to some isometric plant viruses Hendry, Donald Arthur Durham, Tony Microbiology Bibliography: pages 202-212. The binding of divalent cations (particularly calcium and magnesium) to strains of tobacco mosaic virus (TMV) and their isolated proteins was investigated, using equilibrium dialysis and potentiometric titration, in an attempt to elucidate the role of divalent cations in virus stabilisation. It was found that dissociation of bound calcium ions from TMV is apparently a necessary but insufficient condition for in vitro virus disassembly. TMV and the closely related strain, Y-TAMV, possessed three groups per protein subunit which titrated near neutral pH and which showed significant metal ion binding. The tightest of the three calcium binding sites, which was absent on the RNA-free protein, had a computed pKH of 8.3 and pKCa of 5.2 and had a significantly higher affinity for Ca⁺² over Mg⁺² This group thus had some of the characteristics to be expected for a calcium-mediated switch controlling in vivo virus disassembly, and possibly controlled the in vitro alkaline degradation of TMV as well. Both the U2 and cowpea strains of TMV bound one additional metal ion per protein subunit relative to vulgare, this binding site being retained by the polymerised proteins. However, calcium ions stabilised the polymerised forms of the proteins of all four TMV strains at pH values where depolymerisation would normally have occurred. Both bromegrass mosaic virus and turnip crinkle virus bound calcium ions, which stabilised compact forms of these viruses. The phenomenon of cation binding is thus not limited to TMV. In the light of published evidence, it appears that most if not all plant viruses are able to bind divalent cations, which thus represent a hitherto disregarded stabilising element. 2016-03-17T07:12:12Z 2016-03-17T07:12:12Z 1977 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/17879 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Microbiology Hendry, Donald Arthur The binding of divalent cations to tobacco mosaic virus and to some isometric plant viruses |
| thesis_degree_str | Doctoral |
| title | The binding of divalent cations to tobacco mosaic virus and to some isometric plant viruses |
| title_full | The binding of divalent cations to tobacco mosaic virus and to some isometric plant viruses |
| title_fullStr | The binding of divalent cations to tobacco mosaic virus and to some isometric plant viruses |
| title_full_unstemmed | The binding of divalent cations to tobacco mosaic virus and to some isometric plant viruses |
| title_short | The binding of divalent cations to tobacco mosaic virus and to some isometric plant viruses |
| title_sort | binding of divalent cations to tobacco mosaic virus and to some isometric plant viruses |
| topic | Microbiology |
| url | http://hdl.handle.net/11427/17879 |
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