Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Viruses of Heliothis armigera (Hübner) (Lepidoptera : Noctuidae)
Bibliography: pages 249-271.
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Molecular and Cell Biology
2016
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613300845445120 |
|---|---|
| access_status_str | Open Access |
| author | Rubinstein, Riva |
| author2 | Polson, A |
| author_browse | Polson, A Rubinstein, Riva |
| author_facet | Polson, A Rubinstein, Riva |
| author_sort | Rubinstein, Riva |
| collection | Thesis |
| description | Bibliography: pages 249-271. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/18063 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:57.504Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/18063 Viruses of Heliothis armigera (Hübner) (Lepidoptera : Noctuidae) Rubinstein, Riva Polson, A Microbiology Bibliography: pages 249-271. Heliothis armigera (the bollworm) is a pest of agricultural importance in Southern Africa. It is often simultaneously infected with several insect viruses: nuclear polyhedrosis virus (NPV), granulosis virus (GV), and cytoplasmic polyhedrosis virus (CPV). Both NPV and GV have been previously studied because of their ready availability and their potential use in pest control. However, there have been no recorded studies of CPV in H. armigera because of the small amounts of virus present in the naturally infected larvae. Particular emphasis was therefore placed in this study on the CPV of H. armigera. CPV was successfully separated from naturally occurring NPV by a combination of techniques, such as differential centrifugation, density gradient zone electrophoresis and absorption with antibody to NPV. The CPV was then successfully propagated by passage in larvae reared on synthetic media. Pure CPV having been obtained, its replication and physico-chemical properties could be studied. Serological studies using immune electron microscopy and immune osmophoresis were used to detect and identify viruses of H. armigera and examine relationships between different CPVs. The gross morphological appearance of viral infections in H. armigera were studied. Distinctive features were observed both in larvae infected by NPV and in those infected by GV, but CPV-infected larvae were not markedly different in appearance from uninfected larvae. Larvae died readily from NPV or GV infection indicating their suitability for use in controlling H. armigera, but there was no larval mortality following CPV infection. 2016-03-21T19:14:11Z 2016-03-21T19:14:11Z 1977 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/18063 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Microbiology Rubinstein, Riva Viruses of Heliothis armigera (Hübner) (Lepidoptera : Noctuidae) |
| thesis_degree_str | Doctoral |
| title | Viruses of Heliothis armigera (Hübner) (Lepidoptera : Noctuidae) |
| title_full | Viruses of Heliothis armigera (Hübner) (Lepidoptera : Noctuidae) |
| title_fullStr | Viruses of Heliothis armigera (Hübner) (Lepidoptera : Noctuidae) |
| title_full_unstemmed | Viruses of Heliothis armigera (Hübner) (Lepidoptera : Noctuidae) |
| title_short | Viruses of Heliothis armigera (Hübner) (Lepidoptera : Noctuidae) |
| title_sort | viruses of heliothis armigera hubner lepidoptera noctuidae |
| topic | Microbiology |
| url | http://hdl.handle.net/11427/18063 |
| work_keys_str_mv | AT rubinsteinriva virusesofheliothisarmigerahubnerlepidopteranoctuidae |