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The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli
Bibliography: pages 119-137.
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867613217912520704 |
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| access_status_str | Open Access |
| author | Samsodien, Anwar |
| author2 | Abratt, Valerie Rose |
| author_browse | Abratt, Valerie Rose Samsodien, Anwar |
| author_facet | Abratt, Valerie Rose Samsodien, Anwar |
| author_sort | Samsodien, Anwar |
| collection | Thesis |
| description | Bibliography: pages 119-137. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/19682 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:38.580Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/19682 The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli Samsodien, Anwar Abratt, Valerie Rose Molecular and Cell Biology Bibliography: pages 119-137. Butyrivibrio fibrisolvens HI 7C is an important anaerobic bacterium which occurs in the rumen of most ruminants. A key factor affecting the growth of this bacterium is the availability of nitrogen sources, particularly in the form of ammonia. The aims of this study were to attempt the isolation of a gene/genes involved in the regulation of the B.fibrisolvens type III glutamine synthetase (GS), which is a key enzyme involved in ammonia assimilation in rumen bacteria. An existing B. fibrisolvens gene bank, as well as the B. fibrisolvens glnA gene cloned onto a low 11 copy number plasmid, were used to generate a heterologous, Escherichia coli- based, two plasmid, in trans system. This system was used to isolate an E. coli clone which showed increased GS activity levels (2.5-fold) and a retarded growth rate phenotype in complete media. 2016-05-16T11:58:40Z 2016-05-16T11:58:40Z 1997 Master Thesis Masters MSc http://hdl.handle.net/11427/19682 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Molecular and Cell Biology Samsodien, Anwar The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli |
| thesis_degree_str | Master's |
| title | The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli |
| title_full | The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli |
| title_fullStr | The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli |
| title_full_unstemmed | The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli |
| title_short | The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli |
| title_sort | cloning and characterization of a butyrivibrio fibrisolvens h17c glna regulatory element in e coli |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/19682 |
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