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Identification of two potyviruses of phaseolus vulgaris in South Africa
Summary in English.
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867613184994574336 |
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| access_status_str | Open Access |
| author | Van Tonder, Tertia |
| author2 | Rybicki, Edward P |
| author_browse | Rybicki, Edward P Van Tonder, Tertia |
| author_facet | Rybicki, Edward P Van Tonder, Tertia |
| author_sort | Van Tonder, Tertia |
| collection | Thesis |
| description | Summary in English. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/19683 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:07.214Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/19683 Identification of two potyviruses of phaseolus vulgaris in South Africa Van Tonder, Tertia Rybicki, Edward P Molecular and Cell Biology Summary in English. Bibliography: pages 105-125. A survey was conducted by researchers at ARC-PPRI on dry beans (Phaseolus vulgaris) during 1993. All the viruses known to occur on dry beans in South Africa were found, as well as a few unidentified viruses. Of these, samples 93/1 and 93/65 form the basis of this thesis. Electron microscopy (EM) indicated that these viruses could be potyviruses, as they were flexuous particles of approximately 700 to 800 nm. Observation of pinwheels in ultrathin sections of Nicotiana benthamiana infected with isolate 93/1 and Phaseolus vulgaris infected with isolate 93/65, confirmed that the viruses probably belonged to the Potyvirus genus, family Potyviridae. Further serological tests indicated that the viruses were related but not homologous to strains of clover yellow vein (CIYW) and blackeye cowpea mosaic (BICMV) viruses respectively. None of these viruses have previously been described as occurring in South Africa. As we were unable to positively identify the viruses with serological methods, we needed to characterise these viruses on a molecular level. Potyvirus specific oligonucleotide primers were used for PCR amplification of viral eDNA The primers amplify an approximately 700 bp fragment of the virus genome, spanning the 3' noncoding region as well as a part of the coat protein gene: one primer is complementary to the poly(A) tail, and the other to a sequence coding for a conserved block of amino acid sequences (also known as the WCIEN block) in the mid-region of the coat protein. The nucleic acid sequences of the PCR products were compared to that of other potyviruses to positively identify these isolates. 2016-05-16T11:58:48Z 2016-05-16T11:58:48Z 1997 Master Thesis Masters MSc http://hdl.handle.net/11427/19683 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Molecular and Cell Biology Van Tonder, Tertia Identification of two potyviruses of phaseolus vulgaris in South Africa |
| thesis_degree_str | Master's |
| title | Identification of two potyviruses of phaseolus vulgaris in South Africa |
| title_full | Identification of two potyviruses of phaseolus vulgaris in South Africa |
| title_fullStr | Identification of two potyviruses of phaseolus vulgaris in South Africa |
| title_full_unstemmed | Identification of two potyviruses of phaseolus vulgaris in South Africa |
| title_short | Identification of two potyviruses of phaseolus vulgaris in South Africa |
| title_sort | identification of two potyviruses of phaseolus vulgaris in south africa |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/19683 |
| work_keys_str_mv | AT vantondertertia identificationoftwopotyvirusesofphaseolusvulgarisinsouthafrica |