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Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052
Bibliography: pages 117-130.
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867613340407169024 |
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| access_status_str | Open Access |
| author | Leat, Neal |
| author2 | Reid, Sharon J |
| author_browse | Leat, Neal Reid, Sharon J |
| author_facet | Reid, Sharon J Leat, Neal |
| author_sort | Leat, Neal |
| collection | Thesis |
| description | Bibliography: pages 117-130. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/20193 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:33.896Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/20193 Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052 Leat, Neal Reid, Sharon J Woods, David R Molecular and Cell Biology Bibliography: pages 117-130. Solventogenic clostridial strains were used extensively in the industrial acetone-butanolethanol (ABE) fermentation during the first half of this century. This fermentation was based predominantly on two substrates: corn mash (rich in starch) and molasses (rich in sucrose). Taxonomically distinct strains were used for ABE fermentations based on the two substrates. Although starch . utilization by amylolytic solventogenic clostridial strains had been investigated, sucrose utilization by saccharolytic solventogenic clostridial strains had not been comprehensively studied. This provided the impetus for the present study. The primary aim of the project was to provide a molecular and physiological characterization of the sucrose utilization system of Clostridium beijerinckii NCIMB 8052. The C. beijerinckii sucrose utilization operon, scrARBK, was cloned in three stages. In the first stage a C. beijerinckii genomic library was screened in Escherichia coli for clones able to utilize sucrose. Using this approach, a truncated scr operon was isolated. The remainder of the operon was isolated in the second and third stages by recovering a plasmid integrated into the scrB gene, and inverse-PCR respectively. The four genes of the scrARBK operon were prowsed to encode an Enzyme1IBC1ucrose protein of the PTS (ScrA); a transcriptional repressor of the GalR-Lacl family (ScrR), a sucrose-6-phosphate hydrolase of the glucosyl hydrolase family 32 (ScrB) and an ATP-dependent fructokinase of the ribokinase/pfkB sugar kinase family (ScrK). 2016-07-04T08:42:32Z 2016-07-04T08:42:32Z 1997 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/20193 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Molecular and Cell Biology Leat, Neal Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052 |
| thesis_degree_str | Doctoral |
| title | Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052 |
| title_full | Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052 |
| title_fullStr | Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052 |
| title_full_unstemmed | Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052 |
| title_short | Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052 |
| title_sort | studies on the sucrose utilization system of clostridium beijerinckii ncimb 8052 |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/20193 |
| work_keys_str_mv | AT leatneal studiesonthesucroseutilizationsystemofclostridiumbeijerinckiincimb8052 |