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Isolation and characterisation of an Hsp90 homologue from the resurrection plant Xerophyta viscosa
Bibliography: pages 128-163.
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867613864431976448 |
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| access_status_str | Open Access |
| author | Walford, Sally-Ann |
| author2 | Mundree, Sagadevan G |
| author_browse | Mundree, Sagadevan G Walford, Sally-Ann |
| author_facet | Mundree, Sagadevan G Walford, Sally-Ann |
| author_sort | Walford, Sally-Ann |
| collection | Thesis |
| description | Bibliography: pages 128-163. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/20194 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:42:55.472Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/20194 Isolation and characterisation of an Hsp90 homologue from the resurrection plant Xerophyta viscosa Walford, Sally-Ann Mundree, Sagadevan G Thomson, Jennifer Ann Farrant, Jill M Molecular and Cell Biology Bibliography: pages 128-163. Prior to this study, a eDNA library of dehydrated Xerophyta viscosa was differentially screened and several genes were found to be upregulated during dehydration. One of these cDNAs was found to share a high degree of sequence identity with the ER-Iocated Hsp90 or Grp94 family of proteins (hereafter referred to as XVGrp94) and forms the basis of this work. The XVGrp94 eDNA was found to be truncated at the 5· terminus and a full length eDNA was isolated using SMART-RACE™ (§witching Mechanism gt 5' end of RNA Iranscript- Random ~mplification of Complementary .!;rids). This eDNA was sequenced and appeared to be a representative of the Hsp90 family of genes. The putative gene contained an ORF (Open Reading frame) potentially coding for an 812 amino acid protein with a calculated size of 92.83 kDa. It shares 85% homology with other Hsp90s from plants and it contains several characteristic features of these proteins. Additionally, it contains the ER (endoplasmic reticulum) targeting and retention signals. Southern blot analysis confirmed the presence of the gene in the X. viscosa genome possibly as a member of a family of closely related genes. Northern blot analysis revealed a transcript size of 2.8 kb, however, expression patterns of the transcript could not be established. Western blot analysis showed that the XVGrp94 concentration increased significantly in response to heat and dehydration, and a slight increase was observed in response to conditions of high salt, but no response was seen in response to high light, cold or exogenous ABA (abscisic acid) application. The XVGrp94 open reading frame was cloned into the pProEX HTa expression vector and expressed in E. coli, but purification of the recombinant protein was not successful. 2016-07-04T08:42:34Z 2016-07-04T08:42:34Z 2002 Master Thesis Masters MSc http://hdl.handle.net/11427/20194 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Molecular and Cell Biology Walford, Sally-Ann Isolation and characterisation of an Hsp90 homologue from the resurrection plant Xerophyta viscosa |
| thesis_degree_str | Master's |
| title | Isolation and characterisation of an Hsp90 homologue from the resurrection plant Xerophyta viscosa |
| title_full | Isolation and characterisation of an Hsp90 homologue from the resurrection plant Xerophyta viscosa |
| title_fullStr | Isolation and characterisation of an Hsp90 homologue from the resurrection plant Xerophyta viscosa |
| title_full_unstemmed | Isolation and characterisation of an Hsp90 homologue from the resurrection plant Xerophyta viscosa |
| title_short | Isolation and characterisation of an Hsp90 homologue from the resurrection plant Xerophyta viscosa |
| title_sort | isolation and characterisation of an hsp90 homologue from the resurrection plant xerophyta viscosa |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/20194 |
| work_keys_str_mv | AT walfordsallyann isolationandcharacterisationofanhsp90homologuefromtheresurrectionplantxerophytaviscosa |