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Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis
Tuberculosis (TB) is a major global health problem. About 15-20% of the global population who are HIV negative have extra-pulmonary TB (EPTB) such as pleural TB. This increases to 50-70% in HIV positive people. The diagnosis of EPTB is challenging because of the low bacillary burden. Interferon gamm...
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| Format: | Thesis |
| Language: | English |
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Department of Medicine
2016
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| _version_ | 1867613295664431104 |
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| access_status_str | Open Access |
| author | Fanampe, Boitumelo Louisa |
| author2 | Dheda, Keertan |
| author_browse | Dheda, Keertan Fanampe, Boitumelo Louisa |
| author_facet | Dheda, Keertan Fanampe, Boitumelo Louisa |
| author_sort | Fanampe, Boitumelo Louisa |
| collection | Thesis |
| description | Tuberculosis (TB) is a major global health problem. About 15-20% of the global population who are HIV negative have extra-pulmonary TB (EPTB) such as pleural TB. This increases to 50-70% in HIV positive people. The diagnosis of EPTB is challenging because of the low bacillary burden. Interferon gamma (IFN-γ) has been identified as a promising biomarker for the diagnosis of EPTB. The development of rapid and accurate point-of-care (POC) diagnostic technologies becomes crucial in controlling EPTB. Aptamers referred to as "synthetic antibodies" have been recently explored as a replacement for antibodies in diagnostic platforms. These single-stranded nucleic acid molecules have high affinity and specificity comparable, and in some instances even superior, to those of antibodies; in addition to their relatively low cost and simple method of production they have the potential to reduce assay turnaround time. The aim of this thesis was to develop aptamers to IFN-γ, a biomarker specific for EPTB, thus facilitating the development of aptamer-based POC tests for the diagnosis of EPTB. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/20263 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:51.607Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Medicine |
| publisherStr | Department of Medicine |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/20263 Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis Fanampe, Boitumelo Louisa Dheda, Keertan Medicine Tuberculosis (TB) is a major global health problem. About 15-20% of the global population who are HIV negative have extra-pulmonary TB (EPTB) such as pleural TB. This increases to 50-70% in HIV positive people. The diagnosis of EPTB is challenging because of the low bacillary burden. Interferon gamma (IFN-γ) has been identified as a promising biomarker for the diagnosis of EPTB. The development of rapid and accurate point-of-care (POC) diagnostic technologies becomes crucial in controlling EPTB. Aptamers referred to as "synthetic antibodies" have been recently explored as a replacement for antibodies in diagnostic platforms. These single-stranded nucleic acid molecules have high affinity and specificity comparable, and in some instances even superior, to those of antibodies; in addition to their relatively low cost and simple method of production they have the potential to reduce assay turnaround time. The aim of this thesis was to develop aptamers to IFN-γ, a biomarker specific for EPTB, thus facilitating the development of aptamer-based POC tests for the diagnosis of EPTB. 2016-07-08T10:43:27Z 2016-07-08T10:43:27Z 2015 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/20263 eng application/pdf Department of Medicine Faculty of Health Sciences University of Cape Town |
| spellingShingle | Medicine Fanampe, Boitumelo Louisa Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis |
| thesis_degree_str | Doctoral |
| title | Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis |
| title_full | Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis |
| title_fullStr | Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis |
| title_full_unstemmed | Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis |
| title_short | Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis |
| title_sort | interferon γ aptamers for the diagnosis of extra pulmonary tuberculosis |
| topic | Medicine |
| url | http://hdl.handle.net/11427/20263 |
| work_keys_str_mv | AT fanampeboitumelolouisa interferongaptamersforthediagnosisofextrapulmonarytuberculosis |