Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants
Crimean-Congo Hemorrhagic Fever (CCHF) is a highly infectious, life threatening disease, caused by the Crimean-Congo Hemorrhagic Fever Virus (CCHFV), a nairovirus that forms part of the Bunyaviridae family. CCHFV has a case fatality rate of approximately 40%. Current diagnostic methods for CCHFV i...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Molecular and Cell Biology
2016
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613302352248832 |
|---|---|
| access_status_str | Open Access |
| author | Atkinson, Richard |
| author2 | Meyers, Ann |
| author_browse | Atkinson, Richard Meyers, Ann |
| author_facet | Meyers, Ann Atkinson, Richard |
| author_sort | Atkinson, Richard |
| collection | Thesis |
| description | Crimean-Congo Hemorrhagic Fever (CCHF) is a highly infectious, life threatening disease, caused by the Crimean-Congo Hemorrhagic Fever Virus (CCHFV), a nairovirus that forms part of the Bunyaviridae family. CCHFV has a case fatality rate of approximately 40%. Current diagnostic methods for CCHFV involve the use of live virus antigen, requiring biosafety level 4 (BSL4) conditions for safe handling. The development of a safer diagnostic reagent for detection of this disease is therefore desirable. This project involves the development of a recombinant CCHFV nucleocapsid protein (NP). The nucleocapsid (NP) protein was expressed in Nicotiana benthamiana and purified using a 6x histidine-tag. The protein was then reacted against serum samples collected from confirmed CCHFV patients to determine its ability to detect IgG antibodies against CCHFV in human sera. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/20474 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:59.204Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/20474 The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants Atkinson, Richard Meyers, Ann Rybicki, Edward P Molecular and Cell Biology Crimean-Congo Hemorrhagic Fever (CCHF) is a highly infectious, life threatening disease, caused by the Crimean-Congo Hemorrhagic Fever Virus (CCHFV), a nairovirus that forms part of the Bunyaviridae family. CCHFV has a case fatality rate of approximately 40%. Current diagnostic methods for CCHFV involve the use of live virus antigen, requiring biosafety level 4 (BSL4) conditions for safe handling. The development of a safer diagnostic reagent for detection of this disease is therefore desirable. This project involves the development of a recombinant CCHFV nucleocapsid protein (NP). The nucleocapsid (NP) protein was expressed in Nicotiana benthamiana and purified using a 6x histidine-tag. The protein was then reacted against serum samples collected from confirmed CCHFV patients to determine its ability to detect IgG antibodies against CCHFV in human sera. 2016-07-20T06:51:44Z 2016-07-20T06:51:44Z 2016 Master Thesis Masters MSc http://hdl.handle.net/11427/20474 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Molecular and Cell Biology Atkinson, Richard The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants |
| thesis_degree_str | Master's |
| title | The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants |
| title_full | The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants |
| title_fullStr | The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants |
| title_full_unstemmed | The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants |
| title_short | The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants |
| title_sort | production of a crimean congo haemorrhagic fever virus diagnostic antigen in plants |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/20474 |
| work_keys_str_mv | AT atkinsonrichard theproductionofacrimeancongohaemorrhagicfevervirusdiagnosticantigeninplants AT atkinsonrichard productionofacrimeancongohaemorrhagicfevervirusdiagnosticantigeninplants |