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The contribution of respiratory pathogens to Sudden Unexpected Death in Infancy
Background: Sudden unexpected death in infancy (SUDI) is among the most frequent causes of mortality in infants less than one year of age. Respiratory infections have been identified as the most frequent cause of death in these infants. Yet, the broad range of respiratory pathogen that might be invo...
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| Format: | Thesis |
| Language: | English |
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Division of Forensic Medicine and Toxicology
2016
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| _version_ | 1867613266799230976 |
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| access_status_str | Open Access |
| author | Ishimirwe, Elyse Sandrine |
| author2 | Kaba, Mamadou |
| author_browse | Ishimirwe, Elyse Sandrine Kaba, Mamadou |
| author_facet | Kaba, Mamadou Ishimirwe, Elyse Sandrine |
| author_sort | Ishimirwe, Elyse Sandrine |
| collection | Thesis |
| description | Background: Sudden unexpected death in infancy (SUDI) is among the most frequent causes of mortality in infants less than one year of age. Respiratory infections have been identified as the most frequent cause of death in these infants. Yet, the broad range of respiratory pathogen that might be involved in SUDI is poorly studied. This study aimed to investigate the incidence of the respiratory pathogens in SUDI. Methods: A prospective study was carried out on SUDI cases admitted to Salt River Forensic Pathology Laboratory from February 2015 through May 2015. Cerebrospinal fluid, pericardial fluid and lung biopsy were collected from each study participant during post-mortem examination. Total nucleic acids were extracted on the automated QIAsymphony platform. The microbial diversity was investigated using a commercialized multiplex real-time PCR assay, the "FTD Respiratory pathogens 33" kit. This assay is able to detect 21 viruses, 11 bacteria and one fungus. In each real-time PCR run, a positive and non-template sterile water were included as controls. Results: Thirty SUDI cases (median age, 3 (interquartile range (IQR): 2 - 8 months) were included in the study. Twenty participants were males. Positive microbiological results from at least one of the three samples were obtained in 28 cases (93%). According to the type of sample, respiratory pathogens were detected in almost all the lung biopsies (93%), while it was only detected in 60% and 50% of the cerebrospinal and the pericardial fluids, respectively. The median cycle threshold value was lower in lung biopsies (30 (IQR: 28 ‒ 35)) compared to both cerebrospinal (34 (IQR: 30 ‒ 36)) and pericardial fluids (35 (IQR: 33 ‒ 35)) (p= 0.039). In lung biopsies, the most commonly detected bacteria were K. pneumoniae (47%, 14/30) and M. catarrhalis (20%, 6/30). H. influenza (7%, 2/30) and M. pneumoniae (7%, 2/30) were the bacteria often detected in pericardial fluid and cerebrospinal fluid, respectively. Human Metapneumovirus was the most frequently virus detected in all three sample types assessed, accounting for 33% (10/30) in cerebrospinal fluid, 37% (11 /30) in pericardial fluid and 57% (17/30) in lung biopsy samples, respectively. A single type of pathogen was detected n seven of the 28 positive cases. Conclusion: This study highlights the potential implication of respiratory infection in SUDI and it reports one of the highest incidences of respiratory pathogens in SUDI cases. In addition, it is the first to report the high incidence rate of Human Metapneumovirus in SUDI cases. The findings also showed that the majority of SUDI cases are associated with synergetic interaction of multiple respiratory infections. However, data related to histopathology and bacterial culture were not available. A broad range of respiratory pathogens should be included in the routine investigation of SUDI cases with more sensitive diagnostic methods. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/20917 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:25.185Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Division of Forensic Medicine and Toxicology |
| publisherStr | Division of Forensic Medicine and Toxicology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/20917 The contribution of respiratory pathogens to Sudden Unexpected Death in Infancy Ishimirwe, Elyse Sandrine Kaba, Mamadou Heyns, Marise Biomedical Forensic Science Background: Sudden unexpected death in infancy (SUDI) is among the most frequent causes of mortality in infants less than one year of age. Respiratory infections have been identified as the most frequent cause of death in these infants. Yet, the broad range of respiratory pathogen that might be involved in SUDI is poorly studied. This study aimed to investigate the incidence of the respiratory pathogens in SUDI. Methods: A prospective study was carried out on SUDI cases admitted to Salt River Forensic Pathology Laboratory from February 2015 through May 2015. Cerebrospinal fluid, pericardial fluid and lung biopsy were collected from each study participant during post-mortem examination. Total nucleic acids were extracted on the automated QIAsymphony platform. The microbial diversity was investigated using a commercialized multiplex real-time PCR assay, the "FTD Respiratory pathogens 33" kit. This assay is able to detect 21 viruses, 11 bacteria and one fungus. In each real-time PCR run, a positive and non-template sterile water were included as controls. Results: Thirty SUDI cases (median age, 3 (interquartile range (IQR): 2 - 8 months) were included in the study. Twenty participants were males. Positive microbiological results from at least one of the three samples were obtained in 28 cases (93%). According to the type of sample, respiratory pathogens were detected in almost all the lung biopsies (93%), while it was only detected in 60% and 50% of the cerebrospinal and the pericardial fluids, respectively. The median cycle threshold value was lower in lung biopsies (30 (IQR: 28 ‒ 35)) compared to both cerebrospinal (34 (IQR: 30 ‒ 36)) and pericardial fluids (35 (IQR: 33 ‒ 35)) (p= 0.039). In lung biopsies, the most commonly detected bacteria were K. pneumoniae (47%, 14/30) and M. catarrhalis (20%, 6/30). H. influenza (7%, 2/30) and M. pneumoniae (7%, 2/30) were the bacteria often detected in pericardial fluid and cerebrospinal fluid, respectively. Human Metapneumovirus was the most frequently virus detected in all three sample types assessed, accounting for 33% (10/30) in cerebrospinal fluid, 37% (11 /30) in pericardial fluid and 57% (17/30) in lung biopsy samples, respectively. A single type of pathogen was detected n seven of the 28 positive cases. Conclusion: This study highlights the potential implication of respiratory infection in SUDI and it reports one of the highest incidences of respiratory pathogens in SUDI cases. In addition, it is the first to report the high incidence rate of Human Metapneumovirus in SUDI cases. The findings also showed that the majority of SUDI cases are associated with synergetic interaction of multiple respiratory infections. However, data related to histopathology and bacterial culture were not available. A broad range of respiratory pathogens should be included in the routine investigation of SUDI cases with more sensitive diagnostic methods. 2016-07-28T11:10:54Z 2016-07-28T11:10:54Z 2016 Master Thesis Masters MPhil http://hdl.handle.net/11427/20917 eng application/pdf Division of Forensic Medicine and Toxicology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Biomedical Forensic Science Ishimirwe, Elyse Sandrine The contribution of respiratory pathogens to Sudden Unexpected Death in Infancy |
| thesis_degree_str | Master's |
| title | The contribution of respiratory pathogens to Sudden Unexpected Death in Infancy |
| title_full | The contribution of respiratory pathogens to Sudden Unexpected Death in Infancy |
| title_fullStr | The contribution of respiratory pathogens to Sudden Unexpected Death in Infancy |
| title_full_unstemmed | The contribution of respiratory pathogens to Sudden Unexpected Death in Infancy |
| title_short | The contribution of respiratory pathogens to Sudden Unexpected Death in Infancy |
| title_sort | contribution of respiratory pathogens to sudden unexpected death in infancy |
| topic | Biomedical Forensic Science |
| url | http://hdl.handle.net/11427/20917 |
| work_keys_str_mv | AT ishimirweelysesandrine thecontributionofrespiratorypathogenstosuddenunexpecteddeathininfancy AT ishimirweelysesandrine contributionofrespiratorypathogenstosuddenunexpecteddeathininfancy |