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Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli
An atp gene cluster from the extreme acidophile Thiobacillus ferrooxidans ATCC 33020 was cloned by complementation of Escherichia coli unc mutants. Eight different E. coli unc mutants were screened with T. ferrooxidans ATCC 33020 pEcoR251 plasmid and pHC79 cosmid gene banks. The ability of the trans...
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867613449944563713 |
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| access_status_str | Open Access |
| author | Brown, Lynn Dryden |
| author2 | Rawlings, Douglas E |
| author_browse | Brown, Lynn Dryden Rawlings, Douglas E |
| author_facet | Rawlings, Douglas E Brown, Lynn Dryden |
| author_sort | Brown, Lynn Dryden |
| collection | Thesis |
| description | An atp gene cluster from the extreme acidophile Thiobacillus ferrooxidans ATCC 33020 was cloned by complementation of Escherichia coli unc mutants. Eight different E. coli unc mutants were screened with T. ferrooxidans ATCC 33020 pEcoR251 plasmid and pHC79 cosmid gene banks. The ability of the transformants/transductants to grow on succinate as the sole non-fermentable carbon source was used to select mutants with a functional F₁F₀ ATPsynthase. Many F₁ -complementing plasmids and cosmids were isolated from the four E. coli F₁ unc point mutants screened. No plasmids or cosmids which complemented an E. coli fΔunc strain or any of the three E. coli F₀ mutants screened, were isolated. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/21697 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:36:20.187Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/21697 Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli Brown, Lynn Dryden Rawlings, Douglas E Microbiology An atp gene cluster from the extreme acidophile Thiobacillus ferrooxidans ATCC 33020 was cloned by complementation of Escherichia coli unc mutants. Eight different E. coli unc mutants were screened with T. ferrooxidans ATCC 33020 pEcoR251 plasmid and pHC79 cosmid gene banks. The ability of the transformants/transductants to grow on succinate as the sole non-fermentable carbon source was used to select mutants with a functional F₁F₀ ATPsynthase. Many F₁ -complementing plasmids and cosmids were isolated from the four E. coli F₁ unc point mutants screened. No plasmids or cosmids which complemented an E. coli fΔunc strain or any of the three E. coli F₀ mutants screened, were isolated. 2016-09-06T14:44:36Z 2016-09-06T14:44:36Z 1993 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/21697 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Microbiology Brown, Lynn Dryden Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli |
| thesis_degree_str | Doctoral |
| title | Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli |
| title_full | Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli |
| title_fullStr | Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli |
| title_full_unstemmed | Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli |
| title_short | Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli |
| title_sort | studies on thiobacillus ferrooxidans atcc 33020 atp genes and gene products using escherichia coli |
| topic | Microbiology |
| url | http://hdl.handle.net/11427/21697 |
| work_keys_str_mv | AT brownlynndryden studiesonthiobacillusferrooxidansatcc33020atpgenesandgeneproductsusingescherichiacoli |