Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
The characterisation of Ornithogalum mosaic virus
Bibliography: pages 155-179.
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Molecular and Cell Biology
2016
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613308589178880 |
|---|---|
| access_status_str | Open Access |
| author | Burger, Johan Theodorus |
| author2 | Von Wechmar, M Barbara |
| author_browse | Burger, Johan Theodorus Von Wechmar, M Barbara |
| author_facet | Von Wechmar, M Barbara Burger, Johan Theodorus |
| author_sort | Burger, Johan Theodorus |
| collection | Thesis |
| description | Bibliography: pages 155-179. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/21824 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:03.682Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/21824 The characterisation of Ornithogalum mosaic virus Burger, Johan Theodorus Von Wechmar, M Barbara Mosaic diseases Molecular and Cell Biology Bibliography: pages 155-179. Ornithogalum mosaic virus (OMV) is the most serious pathogen of commercially grown Ornithogalum and Lachenalia species in South Africa. Although omithogalum mosaic disease was first reported as early as 1940, attempts to purify or characterise the virus(es) were not successful. The extremely mucilaginous nature of omithogalum and lachenalia plant extracts severely hampered virus purification from these hosts. No alternative propagation host for OMV is known: a virus purification protocol for systemically infected ornithogalum and lachenalia was therefore developed. This method eliminated the mucilage in leaf extracts by hemicellulase digestion. Physicochemical characterisation of purified particles suggested that a single virus was present: it had elongated, filamentous particles with a modal length in the range 720- 760 nm; a single major coat protein of Mᵣ30 000, and a single genomic ssRNA of Mᵣ2.90 x 10⁶ daltons. Oligo(dT)-cellulose chromatography confirmed that the genomic RNA was polyadenylated. 2016-09-20T12:30:58Z 2016-09-20T12:30:58Z 1991 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/21824 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Mosaic diseases Molecular and Cell Biology Burger, Johan Theodorus The characterisation of Ornithogalum mosaic virus |
| thesis_degree_str | Doctoral |
| title | The characterisation of Ornithogalum mosaic virus |
| title_full | The characterisation of Ornithogalum mosaic virus |
| title_fullStr | The characterisation of Ornithogalum mosaic virus |
| title_full_unstemmed | The characterisation of Ornithogalum mosaic virus |
| title_short | The characterisation of Ornithogalum mosaic virus |
| title_sort | characterisation of ornithogalum mosaic virus |
| topic | Mosaic diseases Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/21824 |
| work_keys_str_mv | AT burgerjohantheodorus thecharacterisationofornithogalummosaicvirus AT burgerjohantheodorus characterisationofornithogalummosaicvirus |