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Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize
Bibliography : pages 218-230.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867613184737673216 |
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| access_status_str | Open Access |
| author | Knox, Elizabeth |
| author2 | Von Wechmar, M Barbara |
| author_browse | Knox, Elizabeth Von Wechmar, M Barbara |
| author_facet | Von Wechmar, M Barbara Knox, Elizabeth |
| author_sort | Knox, Elizabeth |
| collection | Thesis |
| description | Bibliography : pages 218-230. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/21898 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:07.214Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/21898 Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize Knox, Elizabeth Von Wechmar, M Barbara Microbiology Bibliography : pages 218-230. Maize plants collected in three geographically distinct regions of South Africa were found to be doubly infected with maize dwarf mosaic (MDMV) and cucumber mosaic virus (CMV). A mixed infection of these two viruses could be maintained in maize plants grown under laboratory conditions. The possibility of synergism or of an interference mechanism between MDMV and CMV in dual infections was investigated and it was found that prior infection with CMV interfered with subsequent infection by MDMV. MDMV and CMV were shown to be non-persistently transmitted by Myzus persicae, Rhopalosiphum padi and Rhopalosipbum maidis aphids. Protoplasts were isolated from maize seedlings and could be viably maintained for up to 66 hours. The maize protoplasts were infected with CMV and MDMV either singly, or together as a mixed inoculum. Infection curves for each virus were plotted. The presence of CMV in a mixed inoculum appeared to prevent infection of the protoplasts by MDMV. Protoplasts were isolated from plants systemically infected with CMV and/or MDMV. Superinfection of protoplasts prepared from CMV infected seedlings with MDMV was not possible. As a possible vehicle for virus infection of protoplasts liposomes were produced. Initially fluorescent dyes were incorporated in them. These were fused to the maize protoplasts. Attempts were made to encapsulate virus particles in the liposomes and fuse them to maize protoplasts but this was not successful. 2016-09-25T16:46:58Z 2016-09-25T16:46:58Z 1986 Master Thesis Masters MSc http://hdl.handle.net/11427/21898 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Microbiology Knox, Elizabeth Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize |
| thesis_degree_str | Master's |
| title | Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize |
| title_full | Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize |
| title_fullStr | Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize |
| title_full_unstemmed | Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize |
| title_short | Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize |
| title_sort | mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize |
| topic | Microbiology |
| url | http://hdl.handle.net/11427/21898 |
| work_keys_str_mv | AT knoxelizabeth mixedinfectionsofmaizedwarfmosaicvirusandcucumbermosaicvirusinmaize |