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Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli
Bibliography: pages 143-154.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867613205282422784 |
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| access_status_str | Open Access |
| author | Scholle, Renate Regina |
| author2 | Woods, David R |
| author_browse | Scholle, Renate Regina Woods, David R |
| author_facet | Woods, David R Scholle, Renate Regina |
| author_sort | Scholle, Renate Regina |
| collection | Thesis |
| description | Bibliography: pages 143-154. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/21906 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:26.116Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/21906 Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli Scholle, Renate Regina Woods, David R Vibrio Escherichia coli Sucrose Bibliography: pages 143-154. This dissertation forms part of the study on the molecular biology of the aerobic, collagenolytic, halotolerant, gram-negative organism Vibrio alginolyticus. The cloning, expression and regulation of the v. alginolyticus sucrose utilization system in Escherichia coli is discussed and the results of a molecular analysis of the sucrase gene (scrA) are presented. The clone pVSlOO, containing a sucrose utilization system, was isolated from a genomic library of v. alginolyticus. Plasmid pVSlOO was mapped and the origin of its insert determined by Southern blotting and DNA hybridization. The number and sizes of the polypeptide products encoded by plasmid pVSlOO were determined by DNAdirected cell-free protein synthesis. The capsule, produced by transformed and untransformed E. coli JA221 cells, was shown to be independent of the presence of plasmid pVSlOO. The sucrase activity assay was optimized with respect to time, pH, temperature and salt requirements. 2016-09-25T16:47:13Z 2016-09-25T16:47:13Z 1989 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/21906 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Vibrio Escherichia coli Sucrose Scholle, Renate Regina Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli |
| thesis_degree_str | Doctoral |
| title | Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli |
| title_full | Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli |
| title_fullStr | Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli |
| title_full_unstemmed | Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli |
| title_short | Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli |
| title_sort | studies on the vibrio alginolyticus sucrose utilization system cloned into escherichia coli |
| topic | Vibrio Escherichia coli Sucrose |
| url | http://hdl.handle.net/11427/21906 |
| work_keys_str_mv | AT schollerenateregina studiesonthevibrioalginolyticussucroseutilizationsystemclonedintoescherichiacoli |