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Fermentation studies on Clostridium acetabutylicum
Bibliography: pages 147-162.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867613162586505216 |
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| access_status_str | Open Access |
| author | Van der Westhuizen, André |
| author2 | Woods, David R |
| author_browse | Van der Westhuizen, André Woods, David R |
| author_facet | Woods, David R Van der Westhuizen, André |
| author_sort | Van der Westhuizen, André |
| collection | Thesis |
| description | Bibliography: pages 147-162. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/22111 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:31:45.395Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/22111 Fermentation studies on Clostridium acetabutylicum Van der Westhuizen, André Woods, David R Jones, David T Microbiology Bibliography: pages 147-162. The initial aim of this work was to develop a laboratory system for the study of the ABE fermentation under laboratory conditions. The development of defined and simple laboratory inoculation and build-up procedures for the ABE process was investigated. A defined spore preparation in distilled water gave solvent yields comparable to the yields obtained in the commercial ABE process. A laboratory inoculation procedure was developed which avoided the lengthy culture build-up procedures presently utilised. An investigation into solvent production by Clostridium acetobutylicum in clostridial basal medium (CBM) , reinforced clostridial medium (RCM) , Leung and Robson media was undertaken with the aim of developing a partially defined laboratory medium which produced solvent yields comparable to the molasses fermentation medium (MFM). The solvent yields obtained in the partially defined laboratory media were substantially lower than those obtained in MFM. It became apparent that the initial aim of trying to identify and manipulate a few key factors to give better solvent yields would not be easily attained. Both the solvent levels and the overall pattern of cell development were markedly different in the various laboratory systems. In view of these differences, a more detailed investigation of the growth patterns, morphological and physiological changes were undertaken. 2016-10-10T14:57:01Z 2016-10-10T14:57:01Z 1982 Master Thesis Masters MSc http://hdl.handle.net/11427/22111 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Microbiology Van der Westhuizen, André Fermentation studies on Clostridium acetabutylicum |
| thesis_degree_str | Master's |
| title | Fermentation studies on Clostridium acetabutylicum |
| title_full | Fermentation studies on Clostridium acetabutylicum |
| title_fullStr | Fermentation studies on Clostridium acetabutylicum |
| title_full_unstemmed | Fermentation studies on Clostridium acetabutylicum |
| title_short | Fermentation studies on Clostridium acetabutylicum |
| title_sort | fermentation studies on clostridium acetabutylicum |
| topic | Microbiology |
| url | http://hdl.handle.net/11427/22111 |
| work_keys_str_mv | AT vanderwesthuizenandre fermentationstudiesonclostridiumacetabutylicum |