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Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus
Bibliography: pages 188-209.
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867613281214005248 |
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| access_status_str | Open Access |
| author | Brand, Reon J |
| author2 | Von Wechmar, M Barbara |
| author_browse | Brand, Reon J Von Wechmar, M Barbara |
| author_facet | Von Wechmar, M Barbara Brand, Reon J |
| author_sort | Brand, Reon J |
| collection | Thesis |
| description | Bibliography: pages 188-209. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/22240 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:37.862Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/22240 Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus Brand, Reon J Von Wechmar, M Barbara Rybicki, Edward P Microbiology Bibliography: pages 188-209. Woodiness disease caused by virus infection is the most serious virus disease of passionfruit and affects economic production of this crop worldwide. A preliminary survey of diseased Passiflora material collected from various regions in South Africa revealed the presence of at least three different viruses. A diseased P. caerulea rootstock specimen from a woodiness diseased vineyard in Natal was selected as a source for isolation and further characterization of viruses. Two viruses that were present in a mixed infection were isolated and purified from this material: a spherical virus which appeared to be cucumber mosaic virus (CMV) and a filamentous virus which was initially presumed to be an isolate of passionfruit woodiness virus (PWV). The host range, transmission and prevalence of these viruses were studied by employing techniques such as electron microscopy (negative staining and immunosorbent), electroblot immunoassay, double antibody sandwich enzyme-linked imunoassay and nucleic acid hybridization. In transmission studies, the CMV-isolate and the potyvirus were found to be sap, aphid and graft transmissible. Separation of the two viruses was achieved by passage through a selective host range. 2016-10-21T07:36:14Z 2016-10-21T07:36:14Z 1992 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/22240 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Microbiology Brand, Reon J Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus |
| thesis_degree_str | Doctoral |
| title | Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus |
| title_full | Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus |
| title_fullStr | Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus |
| title_full_unstemmed | Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus |
| title_short | Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus |
| title_sort | viruses implicated in the woodiness disease of south african passionfruit and the molecular characterization of a new potyvirus |
| topic | Microbiology |
| url | http://hdl.handle.net/11427/22240 |
| work_keys_str_mv | AT brandreonj virusesimplicatedinthewoodinessdiseaseofsouthafricanpassionfruitandthemolecularcharacterizationofanewpotyvirus |